FastQCFastQC Report
Thu 26 May 2016
SRR1547026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547026_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences633422
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26790.42294078828963944No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTCG302.1639516E-645.0000045
CGGAATA302.1639516E-645.00000423
TATATCG351.2107739E-745.00000423
ATAACGG453.8380676E-1045.0000042
ACGCGAT207.030886E-445.037
AACGTAC207.030886E-445.032
TCGTTAG253.8888164E-545.01
TCGCAAA207.030886E-445.036
CGACGCA253.8888164E-545.035
CTATCGT207.030886E-445.021
CGCGATT207.030886E-445.038
CGACAAC207.030886E-445.019
TTATCGG253.8888164E-545.02
CCGCTAG207.030886E-445.019
ACGGGCC406.8066583E-945.05
TCGGCTT207.030886E-445.038
CCGTCAA207.030886E-445.022
AATGCCG207.030886E-445.010
CCATACG207.030886E-445.039
AATCTCG207.030886E-445.018