FastQCFastQC Report
Thu 26 May 2016
SRR1547025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547025_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1344918
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79110.588214300053981No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATA253.891161E-545.02
GCGATAC253.891161E-545.09
CGGTCTA2550.043.23529431
TATAGCG1000.042.751
CACGACG2600.042.4038526
CGTTTTT47150.041.65961
AATGCGG1700.041.0294152
TCACGAC2700.040.83333625
AAATGCG950.040.263161
ACGGGAT4200.040.178575
TAATACG451.9286745E-840.01
TTACGCG451.9286745E-840.01
TACGGGA3700.039.527034
ACGGGTA1200.039.3749965
TATACGG1200.039.3749962
CGGGATT3350.038.9552236
AATACGG1100.038.863642
CGACGGT2900.038.79310228
CTCACGA2850.038.6842124
GTCGTTA356.2490817E-638.57142632
Produced by FastQC (version 0.11.5)