##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547025_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1344918
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37902756896703	31.0	31.0	33.0	30.0	34.0
2	31.77742063084887	31.0	31.0	34.0	30.0	34.0
3	31.89128333474606	31.0	31.0	34.0	30.0	34.0
4	35.66422042087324	37.0	35.0	37.0	33.0	37.0
5	35.57750063572649	37.0	35.0	37.0	33.0	37.0
6	35.63626406963101	37.0	35.0	37.0	33.0	37.0
7	35.95511250500031	37.0	35.0	37.0	35.0	37.0
8	35.93186499102548	37.0	35.0	37.0	35.0	37.0
9	37.67486939724206	39.0	37.0	39.0	35.0	39.0
10	37.13660163667971	39.0	37.0	39.0	33.0	39.0
11	36.891333151909635	39.0	37.0	39.0	33.0	39.0
12	36.37471726900822	38.0	35.0	39.0	32.0	39.0
13	36.182095860119354	39.0	35.0	39.0	32.0	39.0
14	37.22492895477642	39.0	35.0	41.0	32.0	41.0
15	37.410299363976094	39.0	35.0	41.0	32.0	41.0
16	37.48208887084566	39.0	35.0	41.0	32.0	41.0
17	37.418146682548674	39.0	35.0	41.0	32.0	41.0
18	37.281383697742164	39.0	35.0	41.0	32.0	41.0
19	37.24167570067469	39.0	35.0	41.0	32.0	41.0
20	37.09237366144256	39.0	35.0	41.0	32.0	41.0
21	36.981323768437925	39.0	35.0	41.0	32.0	41.0
22	36.896071730767225	39.0	35.0	41.0	31.0	41.0
23	36.858375008736594	38.0	35.0	41.0	31.0	41.0
24	36.768286244960656	38.0	35.0	41.0	31.0	41.0
25	36.68721513133143	38.0	35.0	41.0	31.0	41.0
26	36.56437864613307	38.0	35.0	41.0	31.0	41.0
27	36.46945538687117	38.0	35.0	40.0	31.0	41.0
28	36.37425627436022	38.0	35.0	40.0	30.0	41.0
29	36.405787564743726	38.0	35.0	40.0	30.0	41.0
30	36.36839346339331	38.0	35.0	40.0	30.0	41.0
31	36.29771852261625	38.0	35.0	40.0	30.0	41.0
32	36.18102441933263	38.0	35.0	41.0	30.0	41.0
33	36.06419350473412	38.0	35.0	41.0	30.0	41.0
34	35.925580593017564	38.0	35.0	41.0	29.0	41.0
35	35.8449890625302	38.0	35.0	41.0	29.0	41.0
36	35.74889770231345	38.0	35.0	41.0	29.0	41.0
37	35.72860129762558	38.0	35.0	41.0	29.0	41.0
38	35.667170786620446	38.0	35.0	41.0	29.0	41.0
39	35.59782455138529	38.0	35.0	40.0	28.0	41.0
40	35.469164662827026	38.0	35.0	40.0	27.0	41.0
41	35.40892530250915	38.0	34.0	40.0	27.0	41.0
42	35.36721718350115	38.0	34.0	40.0	27.0	41.0
43	35.27569264445862	38.0	34.0	40.0	27.0	41.0
44	35.160185230623725	38.0	34.0	40.0	27.0	41.0
45	35.055662129587084	37.0	34.0	40.0	26.0	41.0
46	35.0145860193707	37.0	34.0	40.0	26.0	41.0
47	34.98869745218668	37.0	34.0	40.0	26.0	41.0
48	34.89490437335213	37.0	34.0	40.0	26.0	41.0
49	34.81012745758478	37.0	34.0	40.0	26.0	41.0
50	34.72941324303787	37.0	34.0	40.0	26.0	41.0
51	34.36770345850081	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	5.0
11	5.0
12	5.0
13	6.0
14	25.0
15	30.0
16	124.0
17	275.0
18	607.0
19	1125.0
20	1914.0
21	2991.0
22	4580.0
23	6719.0
24	10033.0
25	14226.0
26	19031.0
27	22393.0
28	24414.0
29	27668.0
30	31654.0
31	38005.0
32	47820.0
33	61479.0
34	103078.0
35	154020.0
36	95765.0
37	125851.0
38	190678.0
39	360162.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.28695727174445	23.140146834230784	25.048218553101382	14.524677340923386
2	34.05583091311143	24.726117131304658	27.346351227361072	13.871700728222836
3	29.926434176656123	24.918024742028884	30.607442238114146	14.54809884320085
4	26.76445701522323	26.753006502998694	30.56677061352439	15.91576586825368
5	23.349453275218266	30.717114351953057	30.033355193402127	15.900077179426553
6	22.4264230235598	39.485306910904605	27.538407546036265	10.54986251949933
7	84.06185358512563	4.857768280296643	8.428394890989637	2.651983243588085
8	84.63259470094087	3.9785325201982573	8.555242773165354	2.8336300056955146
9	79.77571866835004	6.211382403982993	10.208429064076768	3.8044698635901963
10	42.547872807115375	29.473618465958516	16.638560863933712	11.33994786299239
11	36.04665860669572	24.55242624457402	24.018192930721426	15.38272221800883
12	32.81902688491046	22.930245561439435	27.52361110491495	16.727116448735167
13	24.21679239923921	29.357551910227986	28.14922545463738	18.276430235895422
14	18.371157200662047	32.9782931003972	30.246899810992193	18.40364988794856
15	17.27458477022391	26.769364377605175	38.52190245055833	17.43414840161259
16	21.46286985526255	24.352488404497524	37.025677401893645	17.158964338346276
17	22.24366095182011	24.15857323643523	28.392660370372024	25.205105441372634
18	23.15248959416113	26.276546228097175	31.891237978821014	18.679726198920676
19	27.150800271838133	26.8710806160673	26.8162073821601	19.161911729934463
20	28.76829665451723	26.354320486453446	27.295716170056465	17.58166668897286
21	25.44571490603888	26.955472378241645	29.651473175316262	17.947339540403206
22	23.97685212035232	22.810312598983728	29.911117257706415	23.301718022957534
23	21.26285766121057	27.074810508893478	29.48268965096757	22.17964217892838
24	21.401527825488245	25.001226840595486	33.94675363107639	19.650491702839876
25	20.88186789083052	26.168658609669883	32.04202784110258	20.907445658397016
26	19.863441488626073	31.54757390413393	27.615735680539633	20.973248926700364
27	19.910953678960354	30.390254275725358	30.173884207066898	19.524907838247387
28	18.243045300903105	27.385833188343085	34.54158543494845	19.829536075805365
29	19.48966405386797	26.328370949009532	33.630228757440975	20.551736239681528
30	20.920531958082204	27.43022251170703	31.47232768094412	20.176917849266644
31	26.10530902255751	25.878752459257736	27.533202767752385	20.48273575043237
32	26.142188594397574	26.54288216828089	27.998063822478397	19.316865414843136
33	25.46519564761569	26.530539408350545	27.402414124876014	20.60185081915775
34	20.976594855597146	27.473868295316144	28.86517988457289	22.68435696451382
35	21.039498318856616	27.092581109034157	30.176114826331418	21.69180574577781
36	25.488617149893155	27.62183270652932	27.25088072283961	19.63866942073792
37	21.785640462838625	30.90032254754565	27.89396825680079	19.420068732814936
38	20.879116793737612	31.677693361230947	25.88484948524743	21.55834035978402
39	21.61410584139702	29.713484390869926	27.352671315277217	21.319738452455837
40	23.49719462450499	27.156376819999434	27.479593551428415	21.866835004067163
41	19.65041734886439	26.303462367222387	29.39160603099966	24.65451425291356
42	21.16597443115491	27.602798088805415	27.05049675891021	24.180730721129468
43	21.90259926627497	26.232528674610645	27.916720573298893	23.94815148581549
44	20.291869095364923	28.283806150263434	29.309742303991765	22.114582450379874
45	20.97577696186682	31.17773723007648	26.20836363257834	21.638122175478355
46	22.006620477977094	29.981009994661385	27.202104514922098	20.81026501243942
47	21.082177500784436	28.2853675837486	28.137923650363817	22.494531265103152
48	22.124992006947636	26.61612083413264	29.883829348703788	21.37505781021594
49	21.196087791225935	26.260708831319086	30.440517563152547	22.102685814302433
50	19.869389806664792	29.513323488866977	29.22996048829743	21.3873262161708
51	19.925675766106185	30.409214539473783	27.27861475569514	22.386494938724887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1233.0
1	2108.5
2	2984.0
3	8521.0
4	14058.0
5	10049.0
6	6040.0
7	6129.5
8	6219.0
9	6357.0
10	6495.0
11	6362.5
12	6230.0
13	6096.5
14	5963.0
15	5886.5
16	5810.0
17	6013.5
18	6217.0
19	6033.0
20	5849.0
21	6536.0
22	7223.0
23	8291.0
24	9359.0
25	9954.0
26	12858.5
27	15168.0
28	17969.0
29	20770.0
30	22840.0
31	24910.0
32	29230.5
33	33551.0
34	38015.0
35	42479.0
36	44850.5
37	47222.0
38	53482.0
39	59742.0
40	69786.5
41	79831.0
42	89169.5
43	98508.0
44	104090.5
45	109673.0
46	108388.0
47	107103.0
48	105012.0
49	102921.0
50	99436.5
51	95952.0
52	86334.0
53	76716.0
54	71460.5
55	66205.0
56	61423.0
57	56641.0
58	54497.0
59	52353.0
60	48608.0
61	44863.0
62	40151.5
63	35440.0
64	30704.0
65	25968.0
66	22811.5
67	19655.0
68	16418.5
69	13182.0
70	10938.5
71	8695.0
72	7258.5
73	5822.0
74	4707.5
75	2849.0
76	2105.0
77	1462.5
78	820.0
79	609.0
80	398.0
81	343.0
82	288.0
83	171.0
84	54.0
85	41.5
86	29.0
87	25.5
88	22.0
89	14.0
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1344918.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.21622240274284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.84531981977116	17.73861382934167
2	5.670561150363087	2.5195689532963925
3	2.0139809623185263	1.3422914692127526
4	1.1483461145816076	1.0204765070748243
5	0.8080045885855723	0.8975404821226902
6	0.6045510046450229	0.8058503743797268
7	0.5207778438105152	0.8098801480360694
8	0.42847393558353614	0.7615257797361878
9	0.40293028756142235	0.8056429993139118
>10	6.443584443101311	35.28014883887212
>50	1.780972242915702	27.600165372828165
>100	0.32945955756368167	9.456356091594579
>500	0.002700488176750683	0.3686670992439714
>1k	0.0	0.0
>5k	3.375610220938354E-4	0.5932720549469197
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7911	0.588214300053981	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4870795096801442E-4	0.0	0.0	0.048330084064604685	0.0
2	1.4870795096801442E-4	0.0	0.0	0.1516077560118907	0.0
3	1.4870795096801442E-4	0.0	0.0	0.22425159005976572	0.0
4	1.4870795096801442E-4	0.0	0.0	0.34210264120191713	0.0
5	1.4870795096801442E-4	0.0	0.0	0.6218966509482362	0.0
6	1.4870795096801442E-4	0.0	0.0	0.9265992424816978	0.0
7	1.4870795096801442E-4	0.0	0.0	1.0998440053594345	0.0
8	1.4870795096801442E-4	0.0	0.0	1.4310166121652026	0.0
9	1.4870795096801442E-4	0.0	0.0	1.558087556267371	0.0
10	1.4870795096801442E-4	0.0	0.0	1.7525232021580497	0.0
11	1.4870795096801442E-4	0.0	0.0	1.9985605070346297	0.0
12	1.4870795096801442E-4	0.0	0.0	2.18950151607756	0.0
13	1.4870795096801442E-4	0.0	0.0	2.2737445703009405	0.0
14	1.4870795096801442E-4	0.0	0.0	2.312111221650688	0.0
15	1.4870795096801442E-4	0.0	0.0	2.364530774366913	0.0
16	1.4870795096801442E-4	0.0	0.0	2.466618782706455	0.0
17	1.4870795096801442E-4	0.0	0.0	2.5794881174911777	0.0
18	1.4870795096801442E-4	0.0	0.0	2.714514936970135	0.0
19	1.4870795096801442E-4	0.0	0.0	2.7993528229973874	0.0
20	1.4870795096801442E-4	0.0	0.0	2.902853556871125	0.0
21	1.4870795096801442E-4	0.0	0.0	3.0303706248261975	0.0
22	1.4870795096801442E-4	0.0	0.0	3.165769214182575	0.0
23	1.4870795096801442E-4	0.0	0.0	3.3115773601067127	0.0
24	1.4870795096801442E-4	0.0	0.0	3.426231190303052	0.0
25	1.4870795096801442E-4	0.0	0.0	3.5277243668387217	0.0
26	1.4870795096801442E-4	0.0	0.0	3.6305559149331037	0.0
27	1.4870795096801442E-4	0.0	0.0	3.7362129140958777	0.0
28	1.4870795096801442E-4	0.0	0.0	3.8509410982677013	0.0
29	1.4870795096801442E-4	0.0	0.0	3.960836274033064	0.0
30	1.4870795096801442E-4	0.0	0.0	4.095342615683633	0.0
31	1.4870795096801442E-4	0.0	0.0	4.225462072780646	0.0
32	1.4870795096801442E-4	0.0	0.0	4.343313123922797	0.0
33	1.4870795096801442E-4	0.0	0.0	4.470978899828837	0.0
34	1.4870795096801442E-4	0.0	0.0	4.5952243928626135	0.0
35	1.4870795096801442E-4	0.0	0.0	4.738950627473199	0.0
36	1.4870795096801442E-4	0.0	0.0	4.8773233758489365	0.0
37	1.4870795096801442E-4	0.0	0.0	5.013316797009185	0.0
38	1.4870795096801442E-4	0.0	0.0	5.168567897819793	0.0
39	1.4870795096801442E-4	0.0	0.0	5.37058764920984	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATA	25	3.891161E-5	45.0	2
GCGATAC	25	3.891161E-5	45.0	9
CGGTCTA	255	0.0	43.235294	31
TATAGCG	100	0.0	42.75	1
CACGACG	260	0.0	42.40385	26
CGTTTTT	4715	0.0	41.6596	1
AATGCGG	170	0.0	41.029415	2
TCACGAC	270	0.0	40.833336	25
AAATGCG	95	0.0	40.26316	1
ACGGGAT	420	0.0	40.17857	5
TAATACG	45	1.9286745E-8	40.0	1
TTACGCG	45	1.9286745E-8	40.0	1
TACGGGA	370	0.0	39.52703	4
ACGGGTA	120	0.0	39.374996	5
TATACGG	120	0.0	39.374996	2
CGGGATT	335	0.0	38.955223	6
AATACGG	110	0.0	38.86364	2
CGACGGT	290	0.0	38.793102	28
CTCACGA	285	0.0	38.68421	24
GTCGTTA	35	6.2490817E-6	38.571426	32
>>END_MODULE