FastQCFastQC Report
Thu 26 May 2016
SRR1547024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547024_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1734937
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83870.4834181298802204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG302.1661435E-645.0000041
CGGTACG302.1661435E-645.00000412
TCGAATA302.1661435E-645.0000041
TAATCGT351.212411E-745.00000421
CTATGCG551.8189894E-1245.01
ATTACCG502.1827873E-1145.01
TCTTACG453.8562575E-1045.01
TAAGTCG207.0342823E-445.01
TACGGGT1650.045.04
ACGGGTA900.045.05
TACGATT406.8193913E-945.033
ATAACGG950.045.02
CGCGTAC253.891631E-545.017
TAGCTAC253.891631E-545.028
ATCGATT207.0342823E-445.017
TACGTAA406.8193913E-945.045
CGGGTGC1400.043.392866
TAGTACG1100.042.9545441
CGTTTTT48850.041.9600831
CGATTCA603.6379788E-1241.25000410