FastQCFastQC Report
Thu 26 May 2016
SRR1547023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547023_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1145752
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47230.4122183509171269No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA253.8907972E-545.00000416
CCTACGG253.8907972E-545.0000042
TCGCGAC253.8907972E-545.00000419
CTATGCG406.8157533E-945.01
GGTACGA207.033277E-445.08
TACCCGA207.033277E-445.039
ACGTTAT207.033277E-445.038
CGCATAA207.033277E-445.027
CGCGCTA207.033277E-445.028
CCTACCG207.033277E-445.01
TTCGTAA207.033277E-445.040
CGTCTAT207.033277E-445.013
ATGTCGC207.033277E-445.035
CCCCGTA207.033277E-445.038
GCCGAAT207.033277E-445.021
TCTACCG207.033277E-445.045
TCCGTAC207.033277E-445.012
CAATCGC207.033277E-445.026
CGTTTCA207.033277E-445.020
ATCGCGT207.033277E-445.030