##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547023_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1145752
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.454092159559835	31.0	31.0	33.0	30.0	34.0
2	31.859108253793142	31.0	31.0	34.0	30.0	34.0
3	31.976749767838065	33.0	31.0	34.0	30.0	34.0
4	35.71976745403892	37.0	35.0	37.0	33.0	37.0
5	35.62903665016513	37.0	35.0	37.0	33.0	37.0
6	35.682039394214456	37.0	35.0	37.0	33.0	37.0
7	36.02842325389788	37.0	35.0	37.0	35.0	37.0
8	36.02387864040386	37.0	35.0	37.0	35.0	37.0
9	37.83028002569492	39.0	38.0	39.0	35.0	39.0
10	37.25009077007939	39.0	37.0	39.0	34.0	39.0
11	36.91968680831454	39.0	37.0	39.0	33.0	39.0
12	36.04772760597407	37.0	35.0	39.0	32.0	39.0
13	35.666867699118136	37.0	35.0	39.0	31.0	39.0
14	36.58253967699817	38.0	35.0	41.0	31.0	41.0
15	36.91215289172526	38.0	35.0	41.0	32.0	41.0
16	37.049741130715894	38.0	35.0	41.0	32.0	41.0
17	36.98971854293076	38.0	35.0	41.0	32.0	41.0
18	36.92373218637192	38.0	35.0	41.0	32.0	41.0
19	36.8943820303172	38.0	35.0	41.0	32.0	41.0
20	36.7364490744943	38.0	35.0	41.0	32.0	41.0
21	36.615126135498784	38.0	35.0	41.0	31.0	41.0
22	36.54371801227491	38.0	35.0	41.0	31.0	41.0
23	36.567449151299755	38.0	35.0	40.0	31.0	41.0
24	36.4590696765094	38.0	35.0	40.0	31.0	41.0
25	36.389876692338305	38.0	35.0	40.0	31.0	41.0
26	36.26199823347461	38.0	35.0	40.0	31.0	41.0
27	36.18447360336268	37.0	35.0	40.0	31.0	41.0
28	36.1556820324119	37.0	35.0	40.0	31.0	41.0
29	36.207493419169246	37.0	35.0	40.0	31.0	41.0
30	36.19527437002074	37.0	35.0	40.0	31.0	41.0
31	36.05724973641765	37.0	35.0	40.0	30.0	41.0
32	35.96670134549187	38.0	35.0	40.0	30.0	41.0
33	35.86473948987216	38.0	35.0	40.0	30.0	41.0
34	35.767213149093344	38.0	35.0	40.0	30.0	41.0
35	35.727437525747284	38.0	35.0	40.0	30.0	41.0
36	35.60528543698811	37.0	35.0	40.0	29.0	41.0
37	35.601459129026175	37.0	34.0	40.0	29.0	41.0
38	35.56756959621279	37.0	34.0	40.0	29.0	41.0
39	35.531455323665156	37.0	35.0	40.0	29.0	41.0
40	35.38434320865248	37.0	34.0	40.0	29.0	41.0
41	35.39498425488238	37.0	34.0	40.0	29.0	41.0
42	35.3399985337141	37.0	34.0	40.0	29.0	41.0
43	35.23896794419735	37.0	34.0	40.0	28.0	41.0
44	35.09259333607971	36.0	34.0	40.0	28.0	41.0
45	35.004495737297425	36.0	34.0	40.0	27.0	41.0
46	35.01320006423729	36.0	34.0	40.0	27.0	41.0
47	34.99624700633296	36.0	34.0	40.0	28.0	41.0
48	34.938982432498484	36.0	34.0	40.0	28.0	41.0
49	34.91328140819305	36.0	34.0	40.0	27.0	41.0
50	34.8160937096335	36.0	34.0	40.0	27.0	41.0
51	34.45680217010313	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	3.0
12	6.0
13	6.0
14	18.0
15	35.0
16	79.0
17	171.0
18	407.0
19	807.0
20	1460.0
21	2358.0
22	3702.0
23	5384.0
24	7851.0
25	11426.0
26	15052.0
27	17794.0
28	19939.0
29	22921.0
30	27327.0
31	33599.0
32	42816.0
33	56547.0
34	105900.0
35	176263.0
36	70738.0
37	92868.0
38	143287.0
39	286743.0
40	240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.53941167023928	24.052587296378274	28.2136099260573	13.194391107325146
2	31.520171904565736	24.356754341253605	30.88879617927789	13.234277574902773
3	29.50856729903155	24.74436003602874	32.0752658515979	13.671806813341805
4	26.184724093870226	27.37197927649264	30.89010536311523	15.553191266521901
5	24.01383545479301	31.53911143074592	29.33976986293718	15.10728325152389
6	22.380148583637645	40.67913475167401	26.449790181470334	10.49092648321801
7	87.8158624204889	3.993097982809543	6.289842828116381	1.9011967685851738
8	88.93355630188732	2.888059545172079	6.318993988227819	1.8593901647127826
9	85.18431562851298	4.743871274062799	7.226869339961876	2.8449437574623477
10	50.643245658746395	24.839668619387094	13.384659158351894	11.132426563514617
11	45.06140944986349	21.749820205419674	20.4320830336757	12.756687311041132
12	41.34428741996523	21.497235003735536	22.855207758747092	14.303269817552142
13	24.62609709605569	34.43380417402719	23.97848749118483	16.961611238732292
14	16.654389431569832	36.93469441903658	28.637349094743016	17.77356705465057
15	17.05098485536137	25.52611734476571	42.346249450142786	15.076648349730135
16	20.69976748895049	21.94453948149338	40.615770253946756	16.73992277560938
17	21.325557363199017	21.2225682346616	27.820331101320356	29.631543300819025
18	24.25428888625113	24.855640662202642	32.04882033808364	18.8412501134626
19	28.981751722885928	26.911495681438915	25.62552803748106	18.481224558194096
20	32.1710108295687	25.701198863279313	25.474535501574515	16.653254805577472
21	25.324939428427797	27.056116856003744	28.671387874513854	18.94755584105461
22	22.663543244960515	23.40270843952269	29.013608529594535	24.92013978592226
23	21.381764989282146	27.373550297097456	28.403267024626622	22.84141768899378
24	22.37822844734288	24.00187824241197	36.029001040364754	17.590892269880392
25	20.76356838129019	25.290202417277037	33.20421871399745	20.742010487435326
26	18.396040329844503	33.6448027147236	26.927555003176952	21.03160195225494
27	17.350787954112235	33.25222212136658	30.374636046893222	19.022353877627967
28	16.539879485263825	29.099752826091514	36.140107108693684	18.220260579950985
29	17.97422129745355	26.822733017267264	34.97929743958553	20.223748245693656
30	20.789402942347035	29.038744859271464	31.314019089645928	18.857833108735573
31	29.050614792730016	26.572417067567848	26.93541010620099	17.441558033501142
32	30.10948268037062	26.18175661050559	26.998338209315804	16.710422499807986
33	28.37341763313527	27.53606365077259	25.06135708251	19.029161633582138
34	20.803891243480265	28.07780392266389	28.449437574623477	22.668867259232364
35	20.898763432226172	27.024871001752558	30.937759654794405	21.13860591122686
36	27.648304345093877	27.692554758795968	26.8797261536528	17.779414742457355
37	20.923288809445676	33.060994002192444	27.75155531039876	18.26416187796312
38	21.494092962525922	32.82961757867322	23.907966121813445	21.76832333698741
39	21.62544774087237	30.92711162625071	26.67322422304303	20.77421640983389
40	25.243857309435203	25.7545262849203	25.960591820917617	23.041024584726888
41	18.828856506469112	25.316386094023834	28.543000579532045	27.311756819975002
42	21.77478197725162	25.44337692624582	25.698755053449613	27.083086043052944
43	21.92944022790272	24.771067386310474	26.632028571628062	26.667463814158737
44	20.130883472165007	29.36726272352132	28.64764800759676	21.854205796716915
45	19.788924653851794	34.44803063839295	24.62321689161354	21.13982781614171
46	22.250277546973514	32.31554472521104	25.459087132293902	19.975090595521543
47	20.526169712119202	29.17603460434719	26.703771845914297	23.59402383761931
48	21.435790642303047	25.812566768375707	31.74255859906856	21.009083990252687
49	21.677291420831036	25.551951905822552	30.834421410567032	21.93633526277938
50	19.773999958106117	31.08395184996404	27.871214713131636	21.270833478798203
51	18.888817126219287	32.61421319796954	26.63455966038026	21.862410015430914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	992.0
1	1670.0
2	2348.0
3	5678.5
4	9009.0
5	6464.5
6	3920.0
7	3947.5
8	3975.0
9	4251.0
10	4527.0
11	4490.0
12	4453.0
13	4353.5
14	4254.0
15	3957.0
16	3660.0
17	3516.5
18	3373.0
19	3474.0
20	3575.0
21	3759.5
22	3944.0
23	5164.0
24	6384.0
25	6516.0
26	8778.0
27	10908.0
28	12221.0
29	13534.0
30	14821.5
31	16109.0
32	19301.5
33	22494.0
34	27306.5
35	32119.0
36	33449.5
37	34780.0
38	42253.0
39	49726.0
40	62906.0
41	76086.0
42	89657.0
43	103228.0
44	107331.5
45	111435.0
46	108616.0
47	105797.0
48	99655.5
49	93514.0
50	87494.5
51	81475.0
52	75451.0
53	69427.0
54	62852.5
55	56278.0
56	52578.5
57	48879.0
58	46363.5
59	43848.0
60	40363.5
61	36879.0
62	31935.5
63	26992.0
64	23012.5
65	19033.0
66	15340.5
67	11648.0
68	9612.0
69	7576.0
70	6453.5
71	5331.0
72	4473.0
73	3615.0
74	2782.5
75	1441.0
76	932.0
77	755.0
78	578.0
79	389.0
80	200.0
81	156.5
82	113.0
83	124.5
84	136.0
85	84.0
86	32.0
87	30.5
88	29.0
89	17.0
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1145752.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.155655176080685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63849829614365	15.446832672778973
2	6.490758975111426	2.486694815165707
3	1.9818678067272728	1.1389192893072886
4	1.01239399136678	0.7757228080383218
5	0.6670061597819638	0.6388469998552537
6	0.49417860808513275	0.5679789007124593
7	0.4059172686472319	0.5442927859756017
8	0.334958213217172	0.5133075224627407
9	0.29270039376082785	0.504618103150691
>10	4.758820829693433	22.60535174034952
>50	1.8193557847519297	24.81739372232615
>100	1.100790549205437	29.26891066255154
>500	0.002294269589840427	0.2759949292640141
>1k	4.5885391796808545E-4	0.4151350480617638
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4723	0.4122183509171269	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03403877977084046	0.0
2	0.0	0.0	0.0	0.11014600018154017	0.0
3	0.0	0.0	0.0	0.15823668647316347	0.0
4	0.0	0.0	0.0	0.246301119264902	0.0
5	0.0	0.0	0.0	0.43988576934624596	0.0
6	0.0	0.0	0.0	0.6127853147976177	0.0
7	0.0	0.0	0.0	0.7204002262269671	0.0
8	0.0	0.0	0.0	0.9431360364197487	0.0
9	0.0	0.0	0.0	1.0060641395345589	0.0
10	0.0	0.0	0.0	1.1433538846102822	0.0
11	0.0	0.0	0.0	1.351775951514813	0.0
12	0.0	0.0	0.0	1.5040776712587016	0.0
13	0.0	0.0	0.0	1.568314958210852	0.0
14	0.0	0.0	0.0	1.5976406761672683	0.0
15	0.0	0.0	0.0	1.6422402055593182	0.0
16	0.0	0.0	0.0	1.7455784497866904	0.0
17	0.0	0.0	0.0	1.8579064230304638	0.0
18	0.0	0.0	0.0	2.007502496177183	0.0
19	0.0	0.0	0.0	2.098534412333559	0.0
20	0.0	0.0	0.0	2.200301635956123	0.0
21	0.0	0.0	0.0	2.334623897667209	0.0
22	0.0	0.0	0.0	2.474182894727655	0.0
23	0.0	0.0	0.0	2.624564478176778	0.0
24	0.0	0.0	0.0	2.7347977572808078	0.0
25	0.0	0.0	0.0	2.8335974975387344	0.0
26	0.0	0.0	0.0	2.9332700270215546	0.0
27	0.0	0.0	0.0	3.0416704487533077	0.0
28	0.0	0.0	0.0	3.1571404632066975	0.0
29	0.0	0.0	0.0	3.2877097312507417	0.0
30	0.0	0.0	0.0	3.4373058043974614	0.0
31	0.0	0.0	0.0	3.5697079298137817	0.0
32	0.0	0.0	0.0	3.691374747763914	0.0
33	0.0	0.0	0.0	3.8230786418003198	0.0
34	0.0	0.0	0.0	3.9682234899000832	0.0
35	0.0	0.0	0.0	4.138155551986817	0.0
36	0.0	0.0	0.0	4.292202850180493	0.0
37	0.0	0.0	0.0	4.450701373421125	0.0
38	0.0	0.0	0.0	4.637740104315768	0.0
39	0.0	0.0	0.0	4.925585990685593	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	3.8907972E-5	45.000004	16
CCTACGG	25	3.8907972E-5	45.000004	2
TCGCGAC	25	3.8907972E-5	45.000004	19
CTATGCG	40	6.8157533E-9	45.0	1
GGTACGA	20	7.033277E-4	45.0	8
TACCCGA	20	7.033277E-4	45.0	39
ACGTTAT	20	7.033277E-4	45.0	38
CGCATAA	20	7.033277E-4	45.0	27
CGCGCTA	20	7.033277E-4	45.0	28
CCTACCG	20	7.033277E-4	45.0	1
TTCGTAA	20	7.033277E-4	45.0	40
CGTCTAT	20	7.033277E-4	45.0	13
ATGTCGC	20	7.033277E-4	45.0	35
CCCCGTA	20	7.033277E-4	45.0	38
GCCGAAT	20	7.033277E-4	45.0	21
TCTACCG	20	7.033277E-4	45.0	45
TCCGTAC	20	7.033277E-4	45.0	12
CAATCGC	20	7.033277E-4	45.0	26
CGTTTCA	20	7.033277E-4	45.0	20
ATCGCGT	20	7.033277E-4	45.0	30
>>END_MODULE