FastQCFastQC Report
Thu 26 May 2016
SRR1547022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547022_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1679658
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237081.4114778127452134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA453.8562575E-1045.08
TATCGCG453.8562575E-1045.01
TAGTACG351.2123746E-745.01
CGTTTTT131700.044.1116181
TACGGGA3650.040.0684934
GCGATAC451.9292202E-840.09
CGGGTAT2600.039.807696
ACGTAAG750.039.0000041
TACGGGT3750.039.04
ACGGGAT4350.037.758625
CTAACCG301.1401461E-437.5000041
TCGTACA601.5643309E-1037.50000434
TAATGCG601.5643309E-1037.5000041
CGTTAGG2100.037.52
TAACGGG3450.037.1739123
GTTTTTT164650.037.1014252
ACGGGTC2550.037.0588235
GTAGGGA16900.037.0118334
CGTAAGG2750.036.8181842
TACGTTG552.750312E-936.818181
Produced by FastQC (version 0.11.5)