##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547022_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1679658
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.410241251492863	31.0	31.0	33.0	30.0	34.0
2	31.829164627561088	31.0	31.0	34.0	30.0	34.0
3	31.84056337659214	31.0	31.0	34.0	30.0	34.0
4	35.672924488199385	37.0	35.0	37.0	33.0	37.0
5	35.60584118909921	37.0	35.0	37.0	33.0	37.0
6	35.681193433425136	37.0	35.0	37.0	33.0	37.0
7	36.02982928667622	37.0	35.0	37.0	35.0	37.0
8	36.02735080593788	37.0	35.0	37.0	35.0	37.0
9	37.80965351279844	39.0	38.0	39.0	35.0	39.0
10	37.135270989689566	39.0	37.0	39.0	33.0	39.0
11	36.9297529616148	39.0	37.0	39.0	33.0	39.0
12	36.558994747740314	39.0	35.0	39.0	32.0	39.0
13	36.44852999836871	39.0	35.0	39.0	32.0	39.0
14	37.48029122595195	40.0	36.0	41.0	32.0	41.0
15	37.65819053640681	40.0	36.0	41.0	32.0	41.0
16	37.71304515562097	40.0	36.0	41.0	33.0	41.0
17	37.62632571630653	40.0	36.0	41.0	32.0	41.0
18	37.43162060371814	39.0	36.0	41.0	32.0	41.0
19	37.31498495527066	39.0	36.0	41.0	32.0	41.0
20	37.09376313511441	39.0	35.0	41.0	32.0	41.0
21	37.02177586151466	39.0	35.0	41.0	32.0	41.0
22	36.96154931539635	39.0	35.0	41.0	32.0	41.0
23	36.90920711240026	38.0	35.0	41.0	32.0	41.0
24	36.81546362414253	38.0	35.0	41.0	32.0	41.0
25	36.73860869296011	38.0	35.0	41.0	31.0	41.0
26	36.62977165589662	38.0	35.0	41.0	31.0	41.0
27	36.539756307534034	38.0	35.0	40.0	31.0	41.0
28	36.42967794634384	38.0	35.0	40.0	31.0	41.0
29	36.417248035016655	38.0	35.0	40.0	31.0	41.0
30	36.31980379339127	38.0	35.0	40.0	30.0	41.0
31	36.13695526113054	38.0	35.0	40.0	30.0	41.0
32	35.947276171696856	38.0	35.0	41.0	29.0	41.0
33	35.689064678642914	38.0	35.0	41.0	27.0	41.0
34	35.431868273184186	38.0	35.0	41.0	25.0	41.0
35	35.254870932058786	38.0	34.0	41.0	24.0	41.0
36	35.13538827547036	38.0	34.0	41.0	24.0	41.0
37	35.1086030608612	38.0	34.0	41.0	24.0	41.0
38	35.03271975604557	38.0	34.0	41.0	23.0	41.0
39	34.94634324368413	38.0	34.0	40.0	23.0	41.0
40	34.82052596421414	38.0	34.0	40.0	23.0	41.0
41	34.7449141432363	38.0	34.0	40.0	23.0	41.0
42	34.68808412188672	38.0	34.0	40.0	23.0	41.0
43	34.59298797731443	38.0	34.0	40.0	23.0	41.0
44	34.47362498794397	38.0	34.0	40.0	22.0	41.0
45	34.382079566197405	37.0	34.0	40.0	23.0	41.0
46	34.36091216188057	37.0	34.0	40.0	23.0	41.0
47	34.34224467123664	37.0	33.0	40.0	23.0	41.0
48	34.23832172978071	37.0	33.0	40.0	22.0	41.0
49	34.14911130718277	37.0	33.0	40.0	22.0	41.0
50	34.08871984654019	37.0	33.0	40.0	22.0	41.0
51	33.7573250030661	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	4.0
12	8.0
13	7.0
14	23.0
15	44.0
16	135.0
17	349.0
18	833.0
19	1573.0
20	2700.0
21	4358.0
22	6686.0
23	10240.0
24	15226.0
25	23524.0
26	33204.0
27	37931.0
28	37806.0
29	37680.0
30	40981.0
31	46733.0
32	56924.0
33	72948.0
34	119313.0
35	161115.0
36	118437.0
37	154588.0
38	237130.0
39	458880.0
40	274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.524964010530713	23.12339773930169	27.81423361184241	18.537404638325185
2	35.815922050798434	24.016853430877	27.563706421188122	12.60351809713644
3	27.18285508121296	23.930228653690218	36.00643702468002	12.880479240416797
4	25.571574689609434	25.46988732230013	35.14935778593023	13.809180202160201
5	22.965448918767986	29.618886701935754	33.727818401126896	13.687845978169367
6	21.179906861992144	38.90411024148964	30.109641367468853	9.806341529049366
7	83.9398258454995	3.0646714986026917	11.430600753248578	1.56490190264923
8	84.70575557643282	2.3501212746880613	11.299919388351675	1.6442037605274407
9	80.48180046176067	4.039512805583041	12.929536846191308	2.549149886464983
10	38.26963584253461	25.041347702925236	25.343730688032924	11.34528576650723
11	31.746700816475737	23.805500881727113	28.597250154495736	15.850548147301415
12	29.967707711927073	21.80908256323609	30.98118783704778	17.24202188778906
13	24.47974528147992	24.67734503095273	31.949242048083597	18.893667639483752
14	18.3205747836762	27.6885532650099	34.7795205928826	19.2113513584313
15	18.474475161014922	26.24236600546064	38.426274872622876	16.856883960901563
16	23.716732811084164	24.40187228590582	35.8580139528404	16.023380950169617
17	23.559379349843837	24.111456022595075	31.825050099484535	20.50411452807655
18	23.46930148875545	25.386477485297604	33.664829387887295	17.479391638059653
19	24.908284900854817	27.085335228957323	30.28616539795601	17.720214472231845
20	25.62855057398589	26.944770899790317	31.200101449223595	16.226577077000197
21	25.24275775187568	25.110826132462684	33.55814100251361	16.088275113148036
22	23.580574140688164	21.200684901331105	34.69706333074947	20.52167762723126
23	21.565461540385005	23.939159043090914	34.895794262879704	19.599585153644373
24	20.94974095917145	24.79915554237827	36.03120397128463	18.21989952716565
25	21.926606487749293	26.179317456291695	32.960043056384094	18.934032999574914
26	19.993415326215217	29.60822977058425	30.81597563313484	19.58237927006569
27	19.267136524221	27.60180941596444	34.013650397878614	19.117403661935942
28	17.898048293164443	27.137905454562773	35.700660491600075	19.26338576067271
29	19.882559425787868	26.147882485601237	34.56150001964686	19.40805806896404
30	21.78080299680054	25.611940049700593	34.11075349862889	18.49650345486998
31	24.229277626754968	24.875480603789583	32.419278210207075	18.47596355924837
32	24.963057955845773	25.765542747392622	31.410560959433408	17.860838337328193
33	24.088237010153257	26.089834954496688	30.426312975617652	19.3956150597324
34	20.720527631220165	26.770925986123366	32.6170565674679	19.891489815188567
35	21.265995815814886	27.405281313219714	31.49075585625169	19.83796701471371
36	23.93653946220004	27.954321653574716	29.368657190928154	18.740481693297088
37	21.308921220867582	29.590785743288215	29.582927000615598	19.5173660352286
38	22.09473595219979	29.85845928159185	27.974980621055	20.071824145153357
39	21.575404040584452	27.99599680411131	28.5418817402114	21.886717415092836
40	23.006528710011203	26.582613841627285	29.577390159187168	20.833467289174344
41	20.469464617201837	26.194915869778253	30.091780588667454	23.24383892435246
42	21.039163925037123	27.41974854404885	29.11568902717101	22.425398503743025
43	20.94444226146037	26.504919453841197	29.974792487518293	22.57584579718014
44	21.393164560880845	27.121771217712176	30.06106004912905	21.42400417227793
45	20.670100699070883	28.668574197842656	28.89671587906586	21.764609224020603
46	21.574868217220413	28.019989783634525	29.5301186312928	20.875023367852265
47	21.13751728030349	27.118734885315938	30.949633794498645	20.79411403988193
48	21.57373703456299	26.69710143374425	30.952789198753557	20.776372332939204
49	21.602314280645228	26.326787953261917	31.47474069125977	20.59615707483309
50	20.610564769732886	28.049400532727496	30.363323962378058	20.976710735161564
51	19.981329532559606	28.667978838549274	30.020754224967227	21.329937403923893
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1382.0
1	2346.5
2	3311.0
3	21378.5
4	39446.0
5	27425.0
6	15404.0
7	15098.5
8	14793.0
9	14932.5
10	15072.0
11	14841.0
12	14610.0
13	13997.5
14	13385.0
15	12505.5
16	11626.0
17	10903.5
18	10181.0
19	9795.5
20	9410.0
21	9766.5
22	10123.0
23	11056.0
24	11989.0
25	13497.5
26	16842.0
27	18678.0
28	22007.0
29	25336.0
30	28758.0
31	32180.0
32	36676.0
33	41172.0
34	46535.0
35	51898.0
36	57711.5
37	63525.0
38	68322.0
39	73119.0
40	82649.5
41	92180.0
42	100454.5
43	108729.0
44	113650.0
45	118571.0
46	123232.5
47	127894.0
48	123974.5
49	120055.0
50	115154.0
51	110253.0
52	102697.5
53	95142.0
54	88420.0
55	81698.0
56	78062.0
57	74426.0
58	70251.5
59	66077.0
60	60707.5
61	55338.0
62	49178.0
63	43018.0
64	37598.5
65	32179.0
66	26912.0
67	21645.0
68	18660.5
69	15676.0
70	12960.0
71	10244.0
72	8522.0
73	6800.0
74	5366.0
75	3117.5
76	2303.0
77	1693.0
78	1083.0
79	762.5
80	442.0
81	301.5
82	161.0
83	127.0
84	93.0
85	60.0
86	27.0
87	22.0
88	17.0
89	16.5
90	16.0
91	12.0
92	8.0
93	5.5
94	3.0
95	1.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1679658.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.43278091000615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.71581495031027	18.988134799235112
2	5.9013984338301615	2.8837514999285156
3	2.337945409591883	1.713675239163392
4	1.4449470708445329	1.4121630083399843
5	1.0194963171269322	1.2454565077460245
6	0.8446957303891776	1.238295942972984
7	0.6909485922949286	1.1817256902933406
8	0.6305802795816897	1.2325463853751881
9	0.5436653844617584	1.1954931504217563
>10	7.537060559927205	42.30656752447235
>50	1.1591430487883412	19.184610433551935
>100	0.17111273727843995	5.48489315839073
>500	0.002945986684316458	0.5106285198319396
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.454988903597048E-4	1.4220581402767092
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23708	1.4114778127452134	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015360269769203017	0.0
2	0.0	0.0	0.0	0.0577498514578563	0.0
3	0.0	0.0	0.0	0.10609302608030921	0.0
4	0.0	0.0	0.0	0.17991757845942447	0.0
5	0.0	0.0	0.0	0.37120651942240623	0.0
6	0.0	0.0	0.0	0.6662665852215154	0.0
7	0.0	0.0	0.0	0.8735706911764181	0.0
8	0.0	0.0	0.0	1.1657730323673035	0.0
9	0.0	0.0	0.0	1.3683737999045045	0.0
10	0.0	0.0	0.0	1.6115780712502188	0.0
11	0.0	0.0	0.0	1.8396602165440823	0.0
12	0.0	0.0	0.0	1.9752235276466996	0.0
13	0.0	0.0	0.0	2.0404153702718055	0.0
14	0.0	0.0	0.0	2.079232796200179	0.0
15	0.0	0.0	0.0	2.1167404316831164	0.0
16	0.0	0.0	0.0	2.185087678563136	0.0
17	0.0	0.0	0.0	2.261353204045109	0.0
18	0.0	0.0	0.0	2.3529194633669475	0.0
19	0.0	0.0	0.0	2.4138247190797175	0.0
20	0.0	0.0	0.0	2.4842557234865668	0.0
21	0.0	0.0	0.0	2.576060126525757	0.0
22	0.0	0.0	0.0	2.6766163111776327	0.0
23	0.0	0.0	0.0	2.7750292023733403	0.0
24	0.0	0.0	0.0	2.8587367190225628	0.0
25	0.0	0.0	0.0	2.937324145748718	0.0
26	0.0	0.0	0.0	3.014601782029437	0.0
27	0.0	0.0	0.0	3.110037876758245	0.0
28	0.0	0.0	0.0	3.1944002886301854	0.0
29	0.0	0.0	0.0	3.3029342878133523	0.0
30	0.0	0.0	0.0	3.4164097691315733	0.0
31	0.0	0.0	0.0	3.515179875903309	0.0
32	0.0	0.0	0.0	3.6152002371911425	0.0
33	0.0	0.0	0.0	3.7250440268197456	0.0
34	5.9535929337996185E-5	0.0	0.0	3.8391148674313462	0.0
35	5.9535929337996185E-5	0.0	0.0	3.9624137770903363	0.0
36	5.9535929337996185E-5	0.0	0.0	4.079223270451485	0.0
37	5.9535929337996185E-5	0.0	0.0	4.208416237114936	0.0
38	5.9535929337996185E-5	0.0	0.0	4.362971509676375	0.0
39	5.9535929337996185E-5	0.0	0.0	4.593911379578461	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	45	3.8562575E-10	45.0	8
TATCGCG	45	3.8562575E-10	45.0	1
TAGTACG	35	1.2123746E-7	45.0	1
CGTTTTT	13170	0.0	44.111618	1
TACGGGA	365	0.0	40.068493	4
GCGATAC	45	1.9292202E-8	40.0	9
CGGGTAT	260	0.0	39.80769	6
ACGTAAG	75	0.0	39.000004	1
TACGGGT	375	0.0	39.0	4
ACGGGAT	435	0.0	37.75862	5
CTAACCG	30	1.1401461E-4	37.500004	1
TCGTACA	60	1.5643309E-10	37.500004	34
TAATGCG	60	1.5643309E-10	37.500004	1
CGTTAGG	210	0.0	37.5	2
TAACGGG	345	0.0	37.173912	3
GTTTTTT	16465	0.0	37.101425	2
ACGGGTC	255	0.0	37.058823	5
GTAGGGA	1690	0.0	37.011833	4
CGTAAGG	275	0.0	36.818184	2
TACGTTG	55	2.750312E-9	36.81818	1
>>END_MODULE