FastQCFastQC Report
Thu 26 May 2016
SRR1547021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547021_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1809159
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167550.9261209213783863No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTT207.0343603E-445.00000427
ATCGTCT207.0343603E-445.00000412
CCGTACG207.0343603E-445.00000420
ATACGCG600.045.0000041
TAAGCGA207.0343603E-445.00000432
TACGTCG207.0343603E-445.00000431
ACGTTAG351.2124474E-745.01
CGGTCTA2100.045.031
ATACCCG253.8916965E-545.016
CGTTTTT85250.043.9442831
ACCGGGT1250.041.3999984
ACGGGTA1750.041.1428575
GAGTACG1150.041.0869561
TCACGAC2250.041.025
TATCGCG556.184564E-1140.9090921
CGCGACC5850.040.76923410
TCTAGCG501.0822987E-940.51
GCGACCT6050.039.7933911
GCGCGAC6000.039.7500049
TCGTACT403.4596815E-739.37500425
Produced by FastQC (version 0.11.5)