FastQCFastQC Report
Thu 26 May 2016
SRR1547020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547020_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1061204
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41460.3906883125204956No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17590.1657551234258446No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA253.8906022E-545.00000431
ATCGTAA253.8906022E-545.00000428
ACCTACT253.8906022E-545.00000421
GTATACG253.8906022E-545.0000041
CGGTCTA850.045.00000431
CAAACGT207.0330413E-445.044
CATGCGG750.045.02
TCCTACG207.0330413E-445.014
CATTACG207.0330413E-445.017
CGTTACC207.0330413E-445.030
ATATACG351.2118107E-745.01
TCGTAAT207.0330413E-445.024
CGTAATG351.2118107E-745.027
CGGTCGA207.0330413E-445.017
CGCACGA453.8562575E-1045.039
CGCAATA351.2118107E-745.033
CGTCCCG351.2118107E-745.037
CCAAACG207.0330413E-445.035
CCGTTCG207.0330413E-445.010
TACACGG406.8139343E-945.02