FastQCFastQC Report
Thu 26 May 2016
SRR1547019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547019_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1268187
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85470.6739542354558121No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG351.2120654E-745.0000041
GTACGAC253.8910337E-545.00000430
TTAGCCG207.033563E-445.01
TAACGCG207.033563E-445.01
TCGATAT207.033563E-445.016
TATAGCG750.045.01
CGTTTTT46750.042.3529431
ATACGGA1100.040.9090924
TCGTAAG501.0822987E-940.5000041
TATACGG950.040.2631532
ATACCGG451.9284926E-840.02
ACGGGAT4200.039.642865
GGCGATA2050.039.5121968
ATAGCGG2000.039.3750042
TACGGGT750.039.04
ATAGGGC3650.038.8356174
TAGGGCA5400.038.755
CGAGATA1800.038.7519
TCGTTAG356.2487998E-638.571431
CGACGGC356.2487998E-638.5714313
Produced by FastQC (version 0.11.5)