##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547019_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1268187
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38251772017849	31.0	31.0	33.0	30.0	34.0
2	31.792979268830226	31.0	31.0	34.0	30.0	34.0
3	31.904509350750324	31.0	31.0	34.0	30.0	34.0
4	35.66548702990963	37.0	35.0	37.0	33.0	37.0
5	35.56660255940173	37.0	35.0	37.0	33.0	37.0
6	35.632099209343735	37.0	35.0	37.0	33.0	37.0
7	35.96930894260862	37.0	35.0	37.0	35.0	37.0
8	35.95475272968419	37.0	35.0	37.0	35.0	37.0
9	37.73436015351049	39.0	37.0	39.0	35.0	39.0
10	37.16302643064469	39.0	37.0	39.0	33.0	39.0
11	36.85095415739161	39.0	37.0	39.0	32.0	39.0
12	36.248438124661426	38.0	35.0	39.0	32.0	39.0
13	36.00913666517635	38.0	35.0	39.0	31.0	39.0
14	36.952650516051655	39.0	35.0	41.0	31.0	41.0
15	37.222891419009976	39.0	35.0	41.0	32.0	41.0
16	37.33339247287663	39.0	35.0	41.0	32.0	41.0
17	37.285304927427894	39.0	35.0	41.0	32.0	41.0
18	37.1944113920108	39.0	35.0	41.0	32.0	41.0
19	37.13047444895745	39.0	35.0	41.0	32.0	41.0
20	36.94396094582266	39.0	35.0	41.0	32.0	41.0
21	36.84362873929476	38.0	35.0	41.0	31.0	41.0
22	36.771868028926335	38.0	35.0	41.0	31.0	41.0
23	36.73254969495824	38.0	35.0	41.0	31.0	41.0
24	36.64781455731686	38.0	35.0	41.0	31.0	41.0
25	36.56985917691949	38.0	35.0	41.0	31.0	41.0
26	36.432684611969684	38.0	35.0	40.0	31.0	41.0
27	36.34740933316616	38.0	35.0	40.0	31.0	41.0
28	36.25678705112101	38.0	35.0	40.0	30.0	41.0
29	36.28453532483774	38.0	35.0	40.0	30.0	41.0
30	36.25155990402046	38.0	35.0	40.0	30.0	41.0
31	36.13701607097376	38.0	35.0	40.0	30.0	41.0
32	36.04165868282832	38.0	35.0	40.0	30.0	41.0
33	35.9155708109293	38.0	35.0	41.0	30.0	41.0
34	35.786768039729154	38.0	35.0	41.0	29.0	41.0
35	35.69882753884088	38.0	35.0	41.0	29.0	41.0
36	35.57844308449779	38.0	35.0	40.0	28.0	41.0
37	35.541741083925324	38.0	34.0	40.0	28.0	41.0
38	35.47460666289751	38.0	34.0	40.0	28.0	41.0
39	35.408890013854425	38.0	34.0	40.0	27.0	41.0
40	35.259598939273154	38.0	34.0	40.0	27.0	41.0
41	35.20098061248065	37.0	34.0	40.0	27.0	41.0
42	35.15937476097768	37.0	34.0	40.0	27.0	41.0
43	35.075581913392895	37.0	34.0	40.0	26.0	41.0
44	34.96980019508164	37.0	34.0	40.0	26.0	41.0
45	34.87317958629129	37.0	34.0	40.0	26.0	41.0
46	34.847128223203676	37.0	34.0	40.0	26.0	41.0
47	34.814630649896266	36.0	34.0	40.0	26.0	41.0
48	34.73325779242336	36.0	34.0	40.0	26.0	41.0
49	34.64927096713655	36.0	34.0	40.0	26.0	41.0
50	34.57126354394107	36.0	34.0	40.0	26.0	41.0
51	34.20711220033008	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	3.0
13	7.0
14	20.0
15	44.0
16	109.0
17	260.0
18	607.0
19	1111.0
20	1974.0
21	2923.0
22	4456.0
23	6647.0
24	9588.0
25	13953.0
26	18374.0
27	21599.0
28	24155.0
29	26533.0
30	31029.0
31	36958.0
32	46295.0
33	61099.0
34	103941.0
35	153348.0
36	89972.0
37	116855.0
38	172061.0
39	324022.0
40	238.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.41724288295023	23.111260405602643	27.982781719099787	14.488714992347344
2	32.007188214356404	24.59992098957015	29.792767155001588	13.60012364107186
3	29.562990316096915	24.59779196601132	31.80674458892892	14.032473128962842
4	27.181874597358274	27.004061703833898	31.003866149077385	14.810197549730441
5	23.825035266880988	31.452774709092584	30.05881624713075	14.663373776895677
6	23.44252070081147	39.78813850007924	26.72910225384742	10.040238545261857
7	85.75643812781554	4.391150516445919	7.7196028661388265	2.13280848959972
8	86.8608493857767	3.4373479620907643	7.412629210045521	2.289173442087011
9	82.81152542960936	5.240473210969675	8.648487959583248	3.299513399837721
10	46.66685591320523	28.50888709630362	14.282278559865382	10.541978430625768
11	38.01521384464594	23.110235320185428	23.85365880583857	15.02089202933006
12	35.213970810298484	22.16423918554598	26.29840867316886	16.323381330986674
13	24.95736038928013	29.473492473901718	27.534582833604194	18.03456430321396
14	17.636594603161836	32.043618173029685	31.044238743970727	19.275548479837752
15	17.334352110532596	26.24297520791492	39.60772346664964	16.81494921490285
16	22.12528593969186	23.616075547218195	35.96685662287975	18.2917818902102
17	21.971917390731807	23.98589482465914	28.253325416519804	25.788862368089248
18	24.207628685674905	25.418885385199502	31.1547114108566	19.218774518268994
19	27.41015323449933	26.278222375722194	26.59008490072836	19.721539489050116
20	28.886433940735866	25.987492380855503	27.42608148482834	17.699992193580286
21	25.691952369800354	26.73856458077555	29.45929898350953	18.11018406591457
22	24.42131956880176	21.946211402577063	29.978859584588076	23.653609444033098
23	20.986731452065037	26.43711061539032	30.167869564977405	22.408288367567245
24	20.63552141758274	25.095116098808774	35.13101774422857	19.138344739379917
25	20.904645765963537	26.34169881886504	32.20455658353224	20.54909883163918
26	19.584414601316684	31.72442234465422	27.312927825312826	21.37823522871627
27	19.36512517475735	30.327940595511542	30.669609450341312	19.63732477938979
28	17.525885378102757	27.86899723778906	34.6399229766588	19.965194407449374
29	18.889091277548182	26.12832334663579	33.82844958984755	21.15413578596847
30	21.426177685152112	28.320744495882703	30.826053255553003	19.42702456341218
31	27.554296014704455	26.140072402571548	27.23754462078542	19.06808696193858
32	27.27137243955347	26.094810938765338	27.439171037078918	19.19464558460227
33	26.39681687322138	26.221369561429032	27.070928814125995	20.310884751223597
34	20.798352293470916	26.870564041422913	29.313894559713987	23.017189105392184
35	21.362858947458065	26.61176939993865	30.763049928756566	21.262321723846718
36	27.279730828339982	26.592687040633596	27.306304196463138	18.821277934563277
37	21.400313991548565	29.725978897433897	28.399676073008163	20.47403103800938
38	21.45314531689727	30.209188392563558	26.591819660665184	21.745846629873984
39	21.332342943114856	28.80064217658752	27.50824602365424	22.358768856643383
40	24.654644780304483	25.88458957551213	27.28170214645001	22.179063497733377
41	19.852198453382663	24.809669236476957	29.23866906063538	26.099463249505
42	21.526005234243843	24.906263823868247	27.712080316230963	25.85565062565694
43	21.504163029584753	25.55774503286976	28.294171127759547	24.64392080978594
44	20.531514674097746	27.61808786874491	29.54974305839754	22.300654398759804
45	20.293458299130965	31.333549389798193	26.459268230947014	21.91372408012383
46	22.106913254906413	29.717699361371785	27.193939064191635	20.981448319530163
47	20.872316148959104	27.242670047871485	29.063300601567434	22.821713201601973
48	21.869251143561634	25.76812410157177	31.233091018911246	21.129533735955345
49	21.712807338349943	25.927722015759507	30.722283070241218	21.63718757564933
50	20.418518720031038	29.149171218440184	29.591219591432495	20.841090470096287
51	19.450601528008093	30.078844839128614	27.848101265822784	22.62245236704051
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1158.0
1	1970.5
2	2783.0
3	8698.5
4	14614.0
5	10247.0
6	5880.0
7	5890.0
8	5900.0
9	6043.5
10	6187.0
11	6116.0
12	6045.0
13	5824.5
14	5604.0
15	5303.0
16	5002.0
17	4738.5
18	4475.0
19	4586.5
20	4698.0
21	4975.5
22	5253.0
23	6114.0
24	6975.0
25	7747.0
26	11178.5
27	13838.0
28	16564.0
29	19290.0
30	21755.5
31	24221.0
32	27588.0
33	30955.0
34	35546.0
35	40137.0
36	42854.5
37	45572.0
38	50375.5
39	55179.0
40	66524.5
41	77870.0
42	86847.0
43	95824.0
44	96963.5
45	98103.0
46	98496.5
47	98890.0
48	94375.0
49	89860.0
50	87249.5
51	84639.0
52	78059.0
53	71479.0
54	67365.5
55	63252.0
56	59877.0
57	56502.0
58	55135.5
59	53769.0
60	47598.5
61	41428.0
62	38797.0
63	36166.0
64	31186.5
65	26207.0
66	23644.0
67	21081.0
68	17954.0
69	14827.0
70	12782.5
71	10738.0
72	8685.5
73	6633.0
74	5527.0
75	3318.5
76	2216.0
77	1547.0
78	878.0
79	756.5
80	635.0
81	465.0
82	295.0
83	209.0
84	123.0
85	75.0
86	27.0
87	20.5
88	14.0
89	14.5
90	15.0
91	9.0
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1268187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.923866725255948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.46377947190811	17.421533106272427
2	5.934001159603277	2.601925011413129
3	2.0896129072909075	1.3743718466046142
4	1.2117794156650072	1.0626756163779258
5	0.8110341718258887	0.8890502546369558
6	0.5953019630886178	0.7830792540022856
7	0.5027564247575748	0.7715655396155867
8	0.4410449250508333	0.7735528125331973
9	0.411329379048085	0.8116137437789251
>10	6.420691680271788	34.94848738415571
>50	1.7366194770803585	26.74953333350803
>100	0.3780617955020621	10.547551234147528
>500	0.0032622781970455673	0.5020827019374017
>1k	3.6247535522728526E-4	0.08375991893669221
>5k	3.6247535522728526E-4	0.6792182420796095
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8547	0.6739542354558121	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.885272440105442E-5	0.0	0.0	0.02554828270594163	0.0
2	7.885272440105442E-5	0.0	0.0	0.08011436799147129	0.0
3	7.885272440105442E-5	0.0	0.0	0.12482386272686914	0.0
4	7.885272440105442E-5	0.0	0.0	0.20517478889154359	0.0
5	7.885272440105442E-5	0.0	0.0	0.38693031863597405	0.0
6	7.885272440105442E-5	0.0	0.0	0.592578223873924	0.0
7	7.885272440105442E-5	0.0	0.0	0.7335668951030092	0.0
8	7.885272440105442E-5	0.0	0.0	1.009945694128705	0.0
9	7.885272440105442E-5	0.0	0.0	1.1250706717542445	0.0
10	7.885272440105442E-5	0.0	0.0	1.3120304813091446	0.0
11	7.885272440105442E-5	0.0	0.0	1.5280080934436324	0.0
12	7.885272440105442E-5	0.0	0.0	1.698408830874311	0.0
13	7.885272440105442E-5	0.0	0.0	1.7672472592764317	0.0
14	7.885272440105442E-5	0.0	0.0	1.8028098379813071	0.0
15	7.885272440105442E-5	0.0	0.0	1.8507522944171482	0.0
16	7.885272440105442E-5	0.0	0.0	1.941511780202762	0.0
17	7.885272440105442E-5	0.0	0.0	2.052851827057051	0.0
18	7.885272440105442E-5	0.0	0.0	2.181933736901577	0.0
19	7.885272440105442E-5	0.0	0.0	2.2684351755695333	0.0
20	7.885272440105442E-5	0.0	0.0	2.3671587865196537	0.0
21	7.885272440105442E-5	0.0	0.0	2.493796261907747	0.0
22	7.885272440105442E-5	0.0	0.0	2.6258745752795134	0.0
23	1.5770544880210885E-4	0.0	0.0	2.757164361407269	0.0
24	1.5770544880210885E-4	0.0	0.0	2.8642463611439006	0.0
25	1.5770544880210885E-4	0.0	0.0	2.962023739401208	0.0
26	1.5770544880210885E-4	0.0	0.0	3.0609050558001303	0.0
27	1.5770544880210885E-4	0.0	0.0	3.163255892072699	0.0
28	1.5770544880210885E-4	0.0	0.0	3.278065458800634	0.0
29	1.5770544880210885E-4	0.0	0.0	3.411247710314015	0.0
30	1.5770544880210885E-4	0.0	0.0	3.576365315209823	0.0
31	1.5770544880210885E-4	0.0	0.0	3.709862977620808	0.0
32	1.5770544880210885E-4	0.0	0.0	3.8275112424271813	0.0
33	1.5770544880210885E-4	0.0	0.0	3.958722175830536	0.0
34	1.5770544880210885E-4	0.0	0.0	4.1065710340825134	0.0
35	1.5770544880210885E-4	0.0	0.0	4.271215522631914	0.0
36	1.5770544880210885E-4	0.0	0.0	4.4048708904917016	0.0
37	1.5770544880210885E-4	0.0	0.0	4.553350570538887	0.0
38	1.5770544880210885E-4	0.0	0.0	4.743701047243033	0.0
39	1.5770544880210885E-4	0.0	0.0	5.104531114102258	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	35	1.2120654E-7	45.000004	1
GTACGAC	25	3.8910337E-5	45.000004	30
TTAGCCG	20	7.033563E-4	45.0	1
TAACGCG	20	7.033563E-4	45.0	1
TCGATAT	20	7.033563E-4	45.0	16
TATAGCG	75	0.0	45.0	1
CGTTTTT	4675	0.0	42.352943	1
ATACGGA	110	0.0	40.909092	4
TCGTAAG	50	1.0822987E-9	40.500004	1
TATACGG	95	0.0	40.263153	2
ATACCGG	45	1.9284926E-8	40.0	2
ACGGGAT	420	0.0	39.64286	5
GGCGATA	205	0.0	39.512196	8
ATAGCGG	200	0.0	39.375004	2
TACGGGT	75	0.0	39.0	4
ATAGGGC	365	0.0	38.835617	4
TAGGGCA	540	0.0	38.75	5
CGAGATA	180	0.0	38.75	19
TCGTTAG	35	6.2487998E-6	38.57143	1
CGACGGC	35	6.2487998E-6	38.57143	13
>>END_MODULE