FastQCFastQC Report
Thu 26 May 2016
SRR1547018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547018_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1857310
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93190.5017471504487673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA3650.042.53424531
CGGGTAT1700.042.352946
CACGACG3750.041.426
TACGCGG1250.041.42
CGTTTTT65450.041.3561
CTATGCG556.184564E-1140.9090921
CACGATA501.0822987E-940.513
CTCACGA3900.040.38461724
GTTAACG1350.040.0000041
CTAACGG451.929402E-840.02
CTACGCG451.929402E-840.01
TACGGGA3900.039.8076934
TCGTCTA403.4597542E-739.37532
AGGGTAC9350.038.9839556
CGCTTAA356.250366E-638.5714342
CGACGGT4050.038.33333628
CGTTAGG3500.037.928572
ACGGTCT4100.037.86585230
TACGGGT2750.037.6363644
CGCGACG301.1401925E-437.50000423
Produced by FastQC (version 0.11.5)