##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547018_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1857310
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43476856313701	31.0	31.0	33.0	30.0	34.0
2	31.851657504670733	31.0	31.0	34.0	30.0	34.0
3	31.96134086393763	33.0	31.0	34.0	30.0	34.0
4	35.71491673441698	37.0	35.0	37.0	33.0	37.0
5	35.62840936623396	37.0	35.0	37.0	33.0	37.0
6	35.69181881322989	37.0	35.0	37.0	33.0	37.0
7	36.03954321034184	37.0	35.0	37.0	35.0	37.0
8	36.03394048381799	37.0	35.0	37.0	35.0	37.0
9	37.82874748964901	39.0	38.0	39.0	35.0	39.0
10	37.149413937361025	39.0	37.0	39.0	33.0	39.0
11	36.91819405484276	39.0	37.0	39.0	33.0	39.0
12	36.41687440438053	39.0	35.0	39.0	32.0	39.0
13	36.256161868508755	39.0	35.0	39.0	32.0	39.0
14	37.284901281961545	39.0	35.0	41.0	32.0	41.0
15	37.47857923556111	39.0	35.0	41.0	32.0	41.0
16	37.55100333277697	39.0	35.0	41.0	33.0	41.0
17	37.48505203762431	39.0	35.0	41.0	32.0	41.0
18	37.36274127636205	39.0	35.0	41.0	32.0	41.0
19	37.32821715276394	39.0	35.0	41.0	32.0	41.0
20	37.18320689599474	39.0	35.0	41.0	32.0	41.0
21	37.086353920454854	39.0	35.0	41.0	32.0	41.0
22	36.99964841625792	39.0	35.0	41.0	32.0	41.0
23	36.96844199406669	39.0	35.0	41.0	32.0	41.0
24	36.88508326558302	38.0	35.0	41.0	32.0	41.0
25	36.804984089893445	38.0	35.0	41.0	31.0	41.0
26	36.680650510684806	38.0	35.0	41.0	31.0	41.0
27	36.59232653676554	38.0	35.0	41.0	31.0	41.0
28	36.52750967797514	38.0	35.0	40.0	31.0	41.0
29	36.55899876703404	38.0	35.0	41.0	31.0	41.0
30	36.50577340347061	38.0	35.0	40.0	31.0	41.0
31	36.436966903747894	38.0	35.0	41.0	31.0	41.0
32	36.35194124836457	38.0	35.0	41.0	30.0	41.0
33	36.2401225428173	38.0	35.0	41.0	30.0	41.0
34	36.12241036768229	38.0	35.0	41.0	30.0	41.0
35	36.029162606134676	38.0	35.0	41.0	30.0	41.0
36	35.942153436959906	38.0	35.0	41.0	29.0	41.0
37	35.91947763162854	38.0	35.0	41.0	29.0	41.0
38	35.870827164016774	38.0	35.0	41.0	29.0	41.0
39	35.80126204026253	38.0	35.0	41.0	29.0	41.0
40	35.67441622561662	38.0	35.0	41.0	28.0	41.0
41	35.63405193532582	38.0	35.0	40.0	28.0	41.0
42	35.58054282806855	38.0	35.0	40.0	28.0	41.0
43	35.50577555712294	38.0	35.0	40.0	28.0	41.0
44	35.38006525566545	38.0	34.0	40.0	27.0	41.0
45	35.290674147019075	38.0	34.0	40.0	27.0	41.0
46	35.2539888333127	38.0	34.0	40.0	27.0	41.0
47	35.22380485756282	38.0	34.0	40.0	27.0	41.0
48	35.13447566642079	37.0	34.0	40.0	27.0	41.0
49	35.05995875755797	37.0	34.0	40.0	27.0	41.0
50	34.99162659975987	37.0	34.0	40.0	27.0	41.0
51	34.630034835326356	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	1.0
11	4.0
12	4.0
13	21.0
14	22.0
15	60.0
16	123.0
17	301.0
18	674.0
19	1251.0
20	2216.0
21	3761.0
22	5469.0
23	8376.0
24	12645.0
25	18605.0
26	24712.0
27	29064.0
28	31979.0
29	34993.0
30	41619.0
31	50995.0
32	63626.0
33	83766.0
34	143110.0
35	212442.0
36	128059.0
37	169111.0
38	263866.0
39	526104.0
40	327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.52709563831563	23.432598758419434	29.328221998481673	14.712083604783263
2	31.90840516661193	25.13355336481255	30.207450560218813	12.75059090835671
3	29.231523009083027	25.053329815701204	32.23500654171894	13.480140633496832
4	26.08735213830755	27.50057879406238	31.87496971426418	14.537099353365889
5	23.843838669904326	31.289337805751327	30.54132051192316	14.325503012421189
6	22.223430660471326	40.58412435188525	26.971534100392503	10.220910887250916
7	87.71405958079157	3.6486100866306646	7.014553305587112	1.6227770269906479
8	88.80628435748476	2.802655453316894	6.749331021746505	1.6417291674518526
9	84.88717553881689	4.513947590870668	8.146836015527834	2.4520408547846078
10	42.681081779562916	25.709870727019187	19.296994039767192	12.312053453650709
11	35.8029085074651	25.36060216119011	23.000306895456333	15.836182435888462
12	32.84971275662114	22.433573286096557	27.474196553079455	17.242517404202854
13	24.665187825403407	28.855656837038513	27.745772111279216	18.733383226278868
14	18.64718329196526	31.74515832036655	30.267375936165745	19.340282451502443
15	18.401882292132168	27.145064636490407	37.322902477238586	17.130150594138836
16	21.645390376404585	24.326202949426857	36.664046389671086	17.364360284497472
17	22.18100370966613	24.501779455233645	29.134716337068124	24.1825004980321
18	23.094098454216045	26.221147789006682	31.792861719368332	18.89189203740894
19	26.51108323327824	27.668832881963702	27.25355487236918	18.566529012388884
20	28.547684554543938	26.553133294926536	27.000339200241207	17.898842950288323
21	25.518680241855158	26.50413770452967	30.128680726426932	17.848501327188245
22	23.20167338785663	22.82403045264388	30.834971006455575	23.13932515304392
23	21.95023986302771	26.165475876401896	30.508208107424178	21.37607615314622
24	21.48833528059398	25.144483150362625	33.88292745960556	19.484254109437842
25	21.1913466249576	26.4219758683257	32.07052134538661	20.316156161330095
26	20.19517474196553	31.317119920745597	28.12201517248063	20.365690164808246
27	19.19431866516629	30.624020761208413	30.672962510297154	19.508698063328147
28	18.059182365894763	28.722076551571895	34.05909621980175	19.159644862731582
29	20.081515740506433	26.087567503540065	33.2385546839246	20.5923620720289
30	20.713074284852826	27.251239696119654	32.38883115904184	19.64685485998568
31	25.715039492599512	25.843181805945157	28.89129978301953	19.5504789184358
32	26.252537271645554	26.502414782669558	28.315036262121023	18.930011683563862
33	25.76398124168825	26.26518997905573	28.25624155364479	19.714587225611233
34	21.47665171673011	27.438661289714695	29.304477981597042	21.780209011958153
35	21.148758150228016	27.142749460240882	30.740533351998323	20.967959037532776
36	25.806462033801576	27.16197080724273	28.00501800991757	19.026549149038125
37	21.362131254340955	30.229687020475847	28.769833791881805	19.638347933301386
38	21.57028175156544	30.650241478266953	26.625819060899904	21.153657709267705
39	22.181972853212443	28.52787095315268	27.89981209383463	21.39034409980025
40	24.140288912459418	25.703248246119387	28.77559481185155	21.380868029569648
41	19.956657746956623	26.205372285725055	29.613311725021674	24.224658242296655
42	21.996597229326284	26.394032229407046	28.005717946923237	23.603652594343433
43	21.863770722173467	25.969008943041278	28.50175791871039	23.665462416074863
44	20.937915587597118	27.21877338731822	29.75033785420851	22.09297317087616
45	20.859738008194647	30.784683224663627	26.92221546214687	21.433363304994856
46	21.98647506339814	29.64200914225412	27.87902934889706	20.492486445450677
47	21.70230063909633	28.042599242991205	28.054336648163204	22.20076346974926
48	21.609639747807314	26.40754639774728	31.202330251815795	20.78048360262961
49	21.339894794083918	26.286834184923357	31.14364322595582	21.229627795036908
50	20.78699840091315	28.87277837302335	29.1035960609699	21.2366271650936
51	19.83729156683591	30.31997889420721	28.25726453849923	21.585465000457653
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1357.0
1	2464.5
2	3572.0
3	10721.0
4	17870.0
5	12834.0
6	7798.0
7	7794.5
8	7791.0
9	8030.0
10	8269.0
11	8137.0
12	8005.0
13	7713.5
14	7422.0
15	7450.5
16	7479.0
17	7185.0
18	6891.0
19	7234.5
20	7578.0
21	7792.0
22	8006.0
23	9240.0
24	10474.0
25	11790.5
26	16256.5
27	19406.0
28	23676.0
29	27946.0
30	33060.5
31	38175.0
32	41229.5
33	44284.0
34	51515.0
35	58746.0
36	64296.5
37	69847.0
38	77991.0
39	86135.0
40	99244.5
41	112354.0
42	126763.5
43	141173.0
44	148032.0
45	154891.0
46	156968.5
47	159046.0
48	156839.5
49	154633.0
50	145736.5
51	136840.0
52	125505.5
53	114171.0
54	103111.0
55	92051.0
56	86469.0
57	80887.0
58	74929.5
59	68972.0
60	61830.5
61	54689.0
62	48319.5
63	41950.0
64	36159.5
65	30369.0
66	24433.0
67	18497.0
68	15383.0
69	12269.0
70	10972.5
71	9676.0
72	7873.5
73	6071.0
74	5036.5
75	3253.0
76	2504.0
77	1707.0
78	910.0
79	814.0
80	718.0
81	529.0
82	340.0
83	216.5
84	93.0
85	61.0
86	29.0
87	19.0
88	9.0
89	7.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1857310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.771143017210054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8275110704976	16.581086491532247
2	6.5978203025284285	2.7408853821134027
3	2.2025918661898642	1.3725105198351981
4	1.1478747350506058	0.9537068115031293
5	0.7711891451159587	0.8009240013261771
6	0.5688020921495235	0.7088801762715629
7	0.4444367956085957	0.6462022170587688
8	0.4025204867278526	0.6688648477744983
9	0.32494114967380955	0.607445918284622
>10	5.142925149926845	26.708527674629128
>50	1.8266889270639612	26.86423331547097
>100	0.7387907122362443	20.296462231093173
>500	0.0031260537845538977	0.4120999209800912
>1k	5.210089640923162E-4	0.13392165236682324
>5k	2.605044820461581E-4	0.504248839760172
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9319	0.5017471504487673	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.384130812842229E-5	0.0	0.0	0.015883185897884573	0.0
2	5.384130812842229E-5	0.0	0.0	0.07203967027582903	0.0
3	5.384130812842229E-5	0.0	0.0	0.12195056291087648	0.0
4	5.384130812842229E-5	0.0	0.0	0.20788129068383845	0.0
5	5.384130812842229E-5	0.0	0.0	0.41371661165879686	0.0
6	5.384130812842229E-5	0.0	0.0	0.6450727126866275	0.0
7	5.384130812842229E-5	0.0	0.0	0.7800528721645821	0.0
8	5.384130812842229E-5	0.0	0.0	1.0300380658048467	0.0
9	5.384130812842229E-5	0.0	0.0	1.1284061357554742	0.0
10	5.384130812842229E-5	0.0	0.0	1.3041980067947732	0.0
11	5.384130812842229E-5	0.0	0.0	1.5722738799661877	0.0
12	5.384130812842229E-5	0.0	0.0	1.7721328157388911	0.0
13	5.384130812842229E-5	0.0	0.0	1.8530024605477815	0.0
14	5.384130812842229E-5	0.0	0.0	1.8874608977499718	0.0
15	5.384130812842229E-5	0.0	0.0	1.935056614135497	0.0
16	5.384130812842229E-5	0.0	0.0	2.0487156155945967	0.0
17	5.384130812842229E-5	0.0	0.0	2.17998072481169	0.0
18	5.384130812842229E-5	0.0	0.0	2.3378972815523524	0.0
19	5.384130812842229E-5	0.0	0.0	2.445526056501069	0.0
20	5.384130812842229E-5	0.0	0.0	2.561446392901562	0.0
21	5.384130812842229E-5	0.0	0.0	2.7123097382774013	0.0
22	5.384130812842229E-5	0.0	0.0	2.873187566965127	0.0
23	5.384130812842229E-5	0.0	0.0	3.043487624575327	0.0
24	5.384130812842229E-5	0.0	0.0	3.1802983885296476	0.0
25	5.384130812842229E-5	0.0	0.0	3.3011182839698274	0.0
26	5.384130812842229E-5	0.0	0.0	3.419623003160485	0.0
27	5.384130812842229E-5	0.0	0.0	3.5327435915383	0.0
28	5.384130812842229E-5	0.0	0.0	3.657816950320625	0.0
29	5.384130812842229E-5	0.0	0.0	3.8037268953486496	0.0
30	5.384130812842229E-5	0.0	0.0	3.9612127216242845	0.0
31	5.384130812842229E-5	0.0	0.0	4.110191621215629	0.0
32	5.384130812842229E-5	0.0	0.0	4.255347787929855	0.0
33	5.384130812842229E-5	0.0	0.0	4.405834244148796	0.0
34	5.384130812842229E-5	0.0	0.0	4.5595511788554415	0.0
35	5.384130812842229E-5	0.0	0.0	4.739811878469399	0.0
36	5.384130812842229E-5	0.0	0.0	4.904512440034243	0.0
37	5.384130812842229E-5	0.0	0.0	5.078473706597175	0.0
38	5.384130812842229E-5	0.0	0.0	5.285547377659087	0.0
39	5.384130812842229E-5	0.0	0.0	5.551954170278521	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	365	0.0	42.534245	31
CGGGTAT	170	0.0	42.35294	6
CACGACG	375	0.0	41.4	26
TACGCGG	125	0.0	41.4	2
CGTTTTT	6545	0.0	41.356	1
CTATGCG	55	6.184564E-11	40.909092	1
CACGATA	50	1.0822987E-9	40.5	13
CTCACGA	390	0.0	40.384617	24
GTTAACG	135	0.0	40.000004	1
CTAACGG	45	1.929402E-8	40.0	2
CTACGCG	45	1.929402E-8	40.0	1
TACGGGA	390	0.0	39.807693	4
TCGTCTA	40	3.4597542E-7	39.375	32
AGGGTAC	935	0.0	38.983955	6
CGCTTAA	35	6.250366E-6	38.57143	42
CGACGGT	405	0.0	38.333336	28
CGTTAGG	350	0.0	37.92857	2
ACGGTCT	410	0.0	37.865852	30
TACGGGT	275	0.0	37.636364	4
CGCGACG	30	1.1401925E-4	37.500004	23
>>END_MODULE