FastQCFastQC Report
Thu 26 May 2016
SRR1547017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547017_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2438827
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190410.7807441856269428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT113150.042.1763151
TCGTTAG850.039.7058831
TCGATAG1350.038.3333361
AGGGCGA28250.038.150446
GCGATCG2500.037.89
GGGCGAT62100.037.5000047
TAAGGGA19850.037.292194
CGAATAT2300.037.17391614
GCGATAC1850.036.486499
CACGACC19550.036.02301827
TCGTTAA250.002107570936.0000047
CGATCGA504.8827133E-836.00000410
AACACGT18050.035.90027641
CGTTAGG3700.035.878382
ACACGTG18300.035.28688442
TACGGGA6250.035.284
ATCCTGC12750.035.1176520
TTAGGGA19700.035.0634544
ATTGGGA13750.035.0181854
AGGGTAC10800.035.0000046
Produced by FastQC (version 0.11.5)