##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547017_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2438827
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59532143936409	31.0	31.0	33.0	30.0	34.0
2	31.98310007228885	31.0	31.0	34.0	30.0	34.0
3	32.05129351118386	33.0	31.0	34.0	30.0	34.0
4	35.75796889242246	37.0	35.0	37.0	35.0	37.0
5	35.70854923288942	37.0	35.0	37.0	33.0	37.0
6	35.761313533104236	37.0	35.0	37.0	33.0	37.0
7	36.099306756895835	37.0	35.0	37.0	35.0	37.0
8	36.03133801618565	37.0	35.0	37.0	35.0	37.0
9	37.793877958543185	39.0	38.0	39.0	35.0	39.0
10	37.311492779110615	39.0	37.0	39.0	34.0	39.0
11	37.05766214659752	39.0	37.0	39.0	33.0	39.0
12	36.65399677795924	39.0	35.0	39.0	33.0	39.0
13	36.54138936464128	39.0	35.0	39.0	32.0	39.0
14	37.589535051071685	40.0	36.0	41.0	32.0	41.0
15	37.691940018705715	40.0	36.0	41.0	33.0	41.0
16	37.7614500741545	40.0	36.0	41.0	33.0	41.0
17	37.68921575823131	40.0	36.0	41.0	33.0	41.0
18	37.586500395477	39.0	36.0	41.0	33.0	41.0
19	37.51175462630191	39.0	36.0	41.0	33.0	41.0
20	37.36860671134115	39.0	35.0	41.0	32.0	41.0
21	37.29612596547439	39.0	35.0	41.0	32.0	41.0
22	37.21812781308391	39.0	35.0	41.0	32.0	41.0
23	37.113205651733395	39.0	35.0	41.0	32.0	41.0
24	37.0071792710184	39.0	35.0	41.0	32.0	41.0
25	36.87030445373944	39.0	35.0	41.0	31.0	41.0
26	36.708749739116385	38.0	35.0	41.0	31.0	41.0
27	36.618615834579494	38.0	35.0	41.0	31.0	41.0
28	36.64031069034417	38.0	35.0	41.0	31.0	41.0
29	36.633533251846075	38.0	35.0	41.0	31.0	41.0
30	36.53176834601224	38.0	35.0	41.0	31.0	41.0
31	36.403206951538586	38.0	35.0	41.0	30.0	41.0
32	36.271355450796634	38.0	35.0	41.0	30.0	41.0
33	36.09788763204606	38.0	35.0	41.0	30.0	41.0
34	35.86316290577396	38.0	35.0	41.0	29.0	41.0
35	35.7173395242877	38.0	35.0	41.0	28.0	41.0
36	35.59570564045748	38.0	35.0	41.0	27.0	41.0
37	35.52274433569909	38.0	35.0	41.0	27.0	41.0
38	35.397279511830895	38.0	35.0	40.0	27.0	41.0
39	35.29301094337565	38.0	34.0	40.0	26.0	41.0
40	35.14879325183787	38.0	34.0	40.0	26.0	41.0
41	35.0693714642326	38.0	34.0	40.0	26.0	41.0
42	34.97972918948331	37.0	34.0	40.0	25.0	41.0
43	34.86028939322059	37.0	34.0	40.0	25.0	41.0
44	34.702149844986955	37.0	34.0	40.0	24.0	41.0
45	34.60175362992127	37.0	34.0	40.0	24.0	41.0
46	34.53885617963062	36.0	34.0	40.0	24.0	41.0
47	34.46390457379716	36.0	34.0	40.0	23.0	41.0
48	34.34913997589825	36.0	34.0	40.0	23.0	41.0
49	34.24804957465208	36.0	33.0	40.0	24.0	41.0
50	34.149197134524094	35.0	33.0	40.0	24.0	41.0
51	33.79207668276594	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	4.0
12	4.0
13	15.0
14	17.0
15	80.0
16	204.0
17	493.0
18	1143.0
19	2175.0
20	3681.0
21	5966.0
22	8821.0
23	12888.0
24	18671.0
25	27587.0
26	37271.0
27	43742.0
28	46344.0
29	50316.0
30	56862.0
31	66565.0
32	81167.0
33	104850.0
34	176498.0
35	250473.0
36	192253.0
37	247912.0
38	359159.0
39	643208.0
40	451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.50072514368588	23.961027165928538	26.445008194513182	15.093239495872401
2	34.43557087075057	25.242216852609882	26.319866066760785	14.002346209878766
3	29.969202407550842	25.40836229876084	30.817725078490604	13.804710215197716
4	26.137319293250407	28.090676378439305	30.168191511739046	15.603812816571244
5	23.900014228151484	31.747516326496307	28.92341277179562	15.429056673556591
6	22.797270983140667	40.73614077587299	26.099145203821344	10.367443037164998
7	86.726528777974	3.2833817240829304	8.256797222599225	1.7332922753438438
8	86.18938530695289	3.0620868146859124	8.129235899061312	2.6192919792998848
9	80.54913284132084	4.750152429836146	10.953011427214804	3.747703301628201
10	43.37859963006806	26.320850146402346	16.876268796433695	13.424281427095893
11	34.19488139175104	25.822085781402286	23.939746443679688	16.04328638316699
12	29.406308852575442	22.164261753703727	28.91603217448388	19.513397219236953
13	24.9374391869534	25.14729417051722	31.196103700672495	18.719162941856883
14	22.166598942852445	26.443901104916424	32.199905938387595	19.189594013843543
15	21.70502458763988	22.893710788014072	36.197237442426214	19.204027181919834
16	25.40692718261689	21.382697501708815	33.58528505711968	19.625090258554625
17	24.605230301288284	22.015419707916962	29.385725186739364	23.99362480405539
18	25.65200401668507	21.737745235721928	31.310174932457286	21.30007581513572
19	27.666743069516613	25.08669126592415	26.73609075182454	20.51047491273469
20	28.909348633584912	24.58374456244744	26.028168459673438	20.478738344294204
21	27.468163998512402	24.945024800857134	27.780363264799018	19.806447935831446
22	26.152408514421072	22.298219594911817	28.038151127570753	23.511220763096357
23	24.669564507855622	24.884462899582463	27.67231952081882	22.773653071743098
24	24.407594306607233	23.748670979942407	30.808868361716513	21.034866351733847
25	24.92710635071696	23.682491624047135	28.86055468469063	22.52984734054527
26	22.05187165797328	25.9724859532882	28.281218799037404	23.694423589701113
27	22.882680895364864	24.28204214567085	28.673415539519613	24.161861419444676
28	22.002503662621418	26.12370619154208	29.653107826016363	22.220682319820142
29	23.364592896503115	24.086169293680936	28.675506708757943	23.87373110105801
30	24.375078675117177	24.353592936276332	29.037648016854007	22.233680371752488
31	25.880351496846636	24.762724047257144	26.755485321427063	22.601439134469153
32	26.829373301181263	24.87626223590275	25.481553222102267	22.81281124081372
33	25.82356190086464	25.099238281354108	25.410494471317563	23.66670534646369
34	23.161462457156656	27.13119872791305	27.30078845280949	22.406550362120807
35	22.832041797142644	27.353641730225224	27.40928323329207	22.40503323934006
36	26.21920292009232	27.812673879697087	24.42100239172356	21.54712080848703
37	23.90866592833358	28.03897119393873	25.866164348680737	22.186198529046955
38	24.886759085412784	27.685973625845538	25.6340855665449	21.793181722196778
39	24.35621714865384	26.22945374969196	25.487867733135644	23.926461368518552
40	25.98130166674389	24.496079467711322	26.720960527335478	22.80165833820931
41	22.53247155292278	24.032823976444416	27.151249350609945	26.283455120022865
42	24.00395764029183	25.017354654512193	26.26647974620586	24.71220795899012
43	22.992159755489013	24.154726842043324	27.93281360260486	24.920299799862804
44	23.118162952927783	25.137084344235976	27.492765989551533	24.25198671328471
45	22.251393805300662	25.416849985669337	26.941394367046122	25.39036184198387
46	24.254569922343816	25.200065441296164	26.54460525490328	24.000759381456742
47	23.04911336474461	25.446536388189894	27.979434375624017	23.524915871441475
48	23.621806712817268	24.70941973333902	28.14131547666153	23.527458077182185
49	23.869753779173347	24.144024975941303	28.145579821774973	23.840641423110373
50	22.796573926727888	25.545108365620028	28.161940145816	23.49637756183608
51	22.5432554256616	25.87358594931088	26.988220156657277	24.594938468370245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1216.0
1	2311.5
2	3407.0
3	17825.5
4	32244.0
5	22184.5
6	12125.0
7	12094.5
8	12064.0
9	12429.0
10	12794.0
11	13065.5
12	13337.0
13	13146.0
14	12955.0
15	12740.0
16	12525.0
17	11882.0
18	11239.0
19	11503.0
20	11767.0
21	11756.0
22	11745.0
23	12051.0
24	12357.0
25	13389.0
26	16645.0
27	18869.0
28	21603.5
29	24338.0
30	27288.5
31	30239.0
32	34852.0
33	39465.0
34	44543.5
35	49622.0
36	55114.5
37	60607.0
38	65440.5
39	70274.0
40	81257.5
41	92241.0
42	105776.5
43	119312.0
44	128384.5
45	137457.0
46	152615.5
47	167774.0
48	177989.5
49	188205.0
50	183212.5
51	178220.0
52	168501.5
53	158783.0
54	151416.0
55	144049.0
56	139957.0
57	135865.0
58	130273.5
59	124682.0
60	122480.5
61	120279.0
62	111877.5
63	103476.0
64	93885.5
65	84295.0
66	73768.5
67	63242.0
68	55835.5
69	48429.0
70	42038.5
71	35648.0
72	30644.0
73	25640.0
74	21942.5
75	15208.0
76	12171.0
77	9379.5
78	6588.0
79	5145.0
80	3702.0
81	2673.5
82	1645.0
83	1209.0
84	773.0
85	538.0
86	303.0
87	200.5
88	98.0
89	72.0
90	46.0
91	41.0
92	36.0
93	21.5
94	7.0
95	6.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2438827.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.416454634818336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.54329048490392	18.689157797243787
2	6.654392226568525	3.249533318445964
3	2.4468142008864184	1.7922758380731756
4	1.4411798404457143	1.4075400877943003
5	1.0149580688043192	1.2390838821601742
6	0.7947140987500779	1.1642460443869092
7	0.6684173685993208	1.1424267650270972
8	0.5828920794005962	1.1385726410943664
9	0.5192540299607676	1.1410508219835314
>10	8.281668558433891	50.21566804597472
>50	0.9930380991511245	14.881787711085828
>100	0.05547901460837508	2.257160301043455
>500	0.0022054384056367847	0.35092970462278467
>1k	0.0015268419731331586	0.5418450739106051
>5k	0.0	0.0
>10k+	1.6964910812590652E-4	0.7887219671532629
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19041	0.7807441856269428	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.200663679711599E-5	0.0	0.0	0.06658938907925818	0.0
2	8.200663679711599E-5	0.0	0.0	0.1964058951290928	0.0
3	8.200663679711599E-5	0.0	0.0	0.3119942496946278	0.0
4	8.200663679711599E-5	0.0	0.0	0.5380045407074795	0.0
5	8.200663679711599E-5	0.0	0.0	0.9377458917750213	0.0
6	8.200663679711599E-5	0.0	0.0	1.7404268527451927	0.0
7	8.200663679711599E-5	0.0	0.0	2.1231928299957317	0.0
8	8.200663679711599E-5	0.0	0.0	3.0199764066905934	0.0
9	8.200663679711599E-5	0.0	0.0	3.508325928817419	0.0
10	8.200663679711599E-5	0.0	0.0	4.157900498887375	0.0
11	8.200663679711599E-5	0.0	0.0	4.667202716715864	0.0
12	8.200663679711599E-5	0.0	0.0	5.05374099925907	0.0
13	8.200663679711599E-5	0.0	0.0	5.272739722825769	0.0
14	8.200663679711599E-5	0.0	0.0	5.403253285288378	0.0
15	8.200663679711599E-5	0.0	0.0	5.521711872141813	0.0
16	1.2300995519567398E-4	0.0	0.0	5.706431821527316	0.0
17	1.2300995519567398E-4	0.0	0.0	5.89968046113972	0.0
18	1.2300995519567398E-4	0.0	0.0	6.1558281911755115	0.0
19	1.2300995519567398E-4	0.0	0.0	6.304055187186299	0.0
20	1.2300995519567398E-4	0.0	0.0	6.46437816212466	0.0
21	1.2300995519567398E-4	0.0	0.0	6.655207605951549	0.0
22	1.2300995519567398E-4	0.0	0.0	6.853089620542991	0.0
23	1.2300995519567398E-4	0.0	0.0	7.082339173709328	0.0
24	1.2300995519567398E-4	0.0	0.0	7.2720615279394565	0.0
25	1.2300995519567398E-4	0.0	0.0	7.441200216333508	0.0
26	1.2300995519567398E-4	0.0	0.0	7.601482187953471	0.0
27	1.2300995519567398E-4	0.0	0.0	7.769636796705957	0.0
28	1.2300995519567398E-4	0.0	0.0	7.937996422050436	0.0
29	1.2300995519567398E-4	0.0	0.0	8.122552358162347	0.0
30	1.2300995519567398E-4	0.0	0.0	8.392190179951264	0.0
31	1.2300995519567398E-4	0.0	0.0	8.598846904679995	0.0
32	1.2300995519567398E-4	0.0	0.0	8.81682054528673	0.0
33	1.2300995519567398E-4	0.0	0.0	9.019171921583613	0.0
34	1.6401327359423198E-4	0.0	0.0	9.230298008017789	0.0
35	1.6401327359423198E-4	0.0	0.0	9.46713317508786	0.0
36	1.6401327359423198E-4	0.0	0.0	9.671493713986273	0.0
37	1.6401327359423198E-4	0.0	0.0	9.884751972977172	0.0
38	2.0501659199278997E-4	0.0	0.0	10.137947464088269	0.0
39	2.0501659199278997E-4	0.0	0.0	10.531620324032824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11315	0.0	42.176315	1
TCGTTAG	85	0.0	39.705883	1
TCGATAG	135	0.0	38.333336	1
AGGGCGA	2825	0.0	38.15044	6
GCGATCG	250	0.0	37.8	9
GGGCGAT	6210	0.0	37.500004	7
TAAGGGA	1985	0.0	37.29219	4
CGAATAT	230	0.0	37.173916	14
GCGATAC	185	0.0	36.48649	9
CACGACC	1955	0.0	36.023018	27
TCGTTAA	25	0.0021075709	36.000004	7
CGATCGA	50	4.8827133E-8	36.000004	10
AACACGT	1805	0.0	35.900276	41
CGTTAGG	370	0.0	35.87838	2
ACACGTG	1830	0.0	35.286884	42
TACGGGA	625	0.0	35.28	4
ATCCTGC	1275	0.0	35.11765	20
TTAGGGA	1970	0.0	35.063454	4
ATTGGGA	1375	0.0	35.018185	4
AGGGTAC	1080	0.0	35.000004	6
>>END_MODULE