FastQCFastQC Report
Thu 26 May 2016
SRR1547016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547016_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences554709
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20870.37623330430910623No Hit
GAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT12720.22930942169678156No Hit
AAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT10810.19487695350174594No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT9850.17757058205293225No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9380.16909767103111722No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT8890.16026421060411855No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC7860.1416959162371622No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT7340.1323216317023881No Hit
TAGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT7250.13069915937906182No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC7090.12781476413759285No Hit
GCTATGGGGATTCGGATTTTTTTTAAAGGTGCTTGCTTGTCAATGTAAATA5720.10311712988251498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAG1100.045.0000041
AGCATAG551.8189894E-1245.0000041
AGTTAAG453.8380676E-1045.0000041
CGTCAGG551.8189894E-1245.0000042
AACCGAA253.8881873E-545.037
AATCCGC207.0301275E-445.019
TCGTTAG351.2104101E-745.01
AGTCCAA351.2104101E-745.043
CGAACAT207.0301275E-445.040
GTCGATG406.8048394E-945.01
CGAGTAT253.8881873E-545.033
TTAGACA207.0301275E-445.011
ATTACGA207.0301275E-445.031
CCAATCT207.0301275E-445.031
TAAACGG502.1827873E-1145.02
CACGATC207.0301275E-445.017
CCGCTAT207.0301275E-445.022
TCGATAG207.0301275E-445.01
CCCGTAT207.0301275E-445.034
CGCTAAG207.0301275E-445.029