##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547016_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	554709
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.704989462943633	31.0	31.0	33.0	30.0	34.0
2	32.07770200231112	33.0	31.0	34.0	30.0	34.0
3	32.14471191201152	33.0	31.0	34.0	30.0	34.0
4	35.79854301985365	37.0	35.0	37.0	35.0	37.0
5	35.754049420506966	37.0	35.0	37.0	33.0	37.0
6	35.81397994263659	37.0	35.0	37.0	35.0	37.0
7	36.143687951700805	37.0	35.0	37.0	35.0	37.0
8	36.07941641473277	37.0	35.0	37.0	35.0	37.0
9	37.89637449545618	39.0	38.0	39.0	35.0	39.0
10	37.41290658705736	39.0	37.0	39.0	35.0	39.0
11	37.1490367021267	39.0	37.0	39.0	34.0	39.0
12	36.184285814724475	37.0	35.0	39.0	33.0	39.0
13	35.768487621437544	37.0	35.0	39.0	31.0	39.0
14	36.734410294406615	38.0	35.0	41.0	32.0	41.0
15	37.013891968581724	38.0	35.0	41.0	32.0	41.0
16	37.20040057038916	38.0	35.0	41.0	33.0	41.0
17	37.16934284462664	38.0	35.0	41.0	33.0	41.0
18	37.11971321900312	38.0	35.0	41.0	33.0	41.0
19	37.036864373933	38.0	35.0	41.0	33.0	41.0
20	36.86810922483681	38.0	35.0	41.0	33.0	41.0
21	36.77474675911154	38.0	35.0	41.0	32.0	41.0
22	36.67583363529346	38.0	35.0	41.0	32.0	41.0
23	36.641729267057144	37.0	35.0	41.0	32.0	41.0
24	36.51811310074291	37.0	35.0	40.0	32.0	41.0
25	36.43053925571786	37.0	35.0	40.0	32.0	41.0
26	36.28516753829486	36.0	35.0	40.0	32.0	41.0
27	36.20224478059667	36.0	35.0	40.0	31.0	41.0
28	36.25856440043338	36.0	35.0	40.0	32.0	41.0
29	36.27657564596933	36.0	35.0	40.0	32.0	41.0
30	36.234263370524005	36.0	35.0	40.0	31.0	41.0
31	36.069265146229824	36.0	35.0	40.0	31.0	41.0
32	35.86457403791898	36.0	35.0	40.0	31.0	41.0
33	35.70641002760006	36.0	35.0	40.0	30.0	41.0
34	35.57753705095825	36.0	35.0	40.0	30.0	41.0
35	35.45636360686414	36.0	35.0	40.0	30.0	41.0
36	35.28457443452333	36.0	35.0	40.0	29.0	41.0
37	35.154673892076744	36.0	35.0	40.0	28.0	41.0
38	35.067453385468774	36.0	34.0	40.0	28.0	41.0
39	34.98894014699599	36.0	34.0	40.0	27.0	41.0
40	34.791059816949065	36.0	34.0	40.0	26.0	41.0
41	34.76667946617055	36.0	34.0	40.0	26.0	41.0
42	34.67698919613707	36.0	34.0	40.0	26.0	41.0
43	34.54362016841263	35.0	34.0	40.0	25.0	41.0
44	34.411093023549284	35.0	34.0	40.0	24.0	41.0
45	34.307761366770684	35.0	34.0	40.0	24.0	41.0
46	34.279589838996664	35.0	34.0	40.0	24.0	41.0
47	34.23730099926268	35.0	34.0	40.0	23.0	41.0
48	34.181080530512396	35.0	34.0	40.0	23.0	41.0
49	34.1675527168299	35.0	34.0	40.0	24.0	41.0
50	34.022361274109485	35.0	34.0	40.0	24.0	41.0
51	33.64357888550573	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	0.0
14	5.0
15	16.0
16	32.0
17	107.0
18	280.0
19	485.0
20	810.0
21	1355.0
22	1966.0
23	3109.0
24	4812.0
25	6662.0
26	8350.0
27	9095.0
28	9736.0
29	10826.0
30	12969.0
31	15080.0
32	18393.0
33	24910.0
34	49494.0
35	106614.0
36	33391.0
37	39626.0
38	60620.0
39	135831.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.26989286274425	25.95757415149204	28.96275344369751	12.809779542066199
2	30.62146098224475	27.45782022646108	28.11798618735229	13.802732603941886
3	28.651419032321453	26.05474221618903	31.22069409365992	14.0731446578296
4	23.746685198906093	31.299654413395135	30.066214898261972	14.887445489436804
5	22.964473264360233	33.00974024218103	29.73342779727749	14.292358696181239
6	21.031027079063076	41.487518680966055	26.468833208042415	11.012621031928454
7	86.32634408311385	4.831902853568267	7.118687455945369	1.7230656073725141
8	86.14624965522462	3.748992715099268	6.0992340127886875	4.005523616887413
9	82.21229509526616	5.5969886913679066	9.327953936207994	2.8627622771579335
10	49.72859643524803	20.941070002469765	15.802700154495419	13.527633407786785
11	45.94336850492781	22.398230423519358	19.219446592717983	12.438954478834848
12	40.81220964505714	21.682539854229876	21.952591358712407	15.55265914200058
13	20.031223578488902	43.29873861790596	23.59489389932379	13.075143904281344
14	14.954868228206141	45.2502122734623	25.778921921223564	14.015997577107997
15	13.228557676186975	25.53464969921166	47.21033911474304	14.026453509858323
16	13.966422033895249	19.875285960746986	48.156781303350044	18.00151070200772
17	14.336886547721416	21.918519439922555	26.927632326138568	36.81696168621746
18	18.983647281727897	25.040156190002328	34.18531157778222	21.790884950487552
19	25.228543254210766	27.640618774889177	25.3466231844084	21.784214786491656
20	27.817828807536927	26.21428532798278	24.63634085619667	21.331545008283623
21	20.45342693195892	31.174363495093825	27.874074514745566	20.49813505820169
22	21.52173481951798	26.5968282468826	25.07657167992587	26.804865253673547
23	19.296243616022092	32.59871392027171	22.997283260231942	25.107759203474256
24	18.988514698697877	26.700125651467705	37.229790755152706	17.081568894681716
25	16.600055164058993	26.398526074031608	35.26028962933718	21.741129132572212
26	16.25014196632829	35.094076353547536	27.659367343958724	20.996414336165447
27	18.15312172688743	34.67205327478011	27.131523014769904	20.043301983562554
28	14.521668117878022	31.486419005280247	37.412228754175615	16.57968412266612
29	14.25468128333955	26.3128955903005	36.42955135034766	23.002871776012288
30	17.28618068212342	30.64381504536613	32.88462959858232	19.18537467392813
31	26.336151026934846	28.96311399310269	24.854112696927576	19.84662228303489
32	27.109709775756297	27.228330530061704	27.22274201428136	18.439217679900633
33	25.122541729086784	29.32780971644592	24.888905714527798	20.6607428399395
34	17.55605191190336	27.788444031014457	29.664923410292605	24.990580646789578
35	18.184669799840997	26.762680973267067	31.25981370412234	23.792835522769597
36	28.88613669509599	24.250552992650203	26.116215889772835	20.747094422480977
37	20.312632389234718	30.0391736928732	30.237295591021603	19.410898326870484
38	20.519587747810114	30.747472999356418	24.96480136431895	23.768137888514516
39	20.691389539380108	30.136702306975373	27.202370972888488	21.969537180756035
40	23.27580767573629	25.454066907153123	25.955050305655757	25.31507511145483
41	15.948722663594786	23.98753220156875	28.532798278016042	31.530946856820425
42	22.192717262564695	26.4295333228774	25.52888090872872	25.84886850582918
43	21.802422531453427	26.439808980925132	27.61916608528075	24.138602402340688
44	20.353915296128243	29.68817884692695	27.550661698295865	22.407244158648947
45	17.775085675552408	33.527308913322116	24.04540038110072	24.652205030024753
46	23.121672805020292	29.192062865394284	27.39201996001507	20.294244369570354
47	21.41897823904065	26.005707497084057	28.904164165355166	23.671150098520126
48	22.77698757366475	22.98844979980494	30.42568265523004	23.808879971300268
49	21.730492925119297	21.33316748060695	32.21256550732006	24.7237740869537
50	19.85888096281113	29.269400712806178	27.725888709215102	23.145829615167592
51	17.661692887622156	31.011395163950827	24.591813004656498	26.73509894377052
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	625.0
1	702.5
2	780.0
3	2150.5
4	3521.0
5	2726.0
6	1931.0
7	2240.5
8	2550.0
9	2938.0
10	3326.0
11	3588.0
12	3850.0
13	3936.0
14	4022.0
15	3880.5
16	3739.0
17	3625.0
18	3511.0
19	3467.0
20	3423.0
21	3342.5
22	3262.0
23	3160.5
24	3059.0
25	3268.0
26	3787.5
27	4098.0
28	4179.0
29	4260.0
30	5289.5
31	6319.0
32	7080.0
33	7841.0
34	9112.0
35	10383.0
36	11052.5
37	11722.0
38	14620.5
39	17519.0
40	26051.5
41	34584.0
42	44074.0
43	53564.0
44	60364.0
45	67164.0
46	65767.5
47	64371.0
48	59359.5
49	54348.0
50	47785.5
51	41223.0
52	37653.5
53	34084.0
54	29661.5
55	25239.0
56	22548.5
57	19858.0
58	18222.5
59	16587.0
60	14673.5
61	12760.0
62	11238.0
63	9716.0
64	8144.0
65	6572.0
66	5275.5
67	3979.0
68	3174.0
69	2369.0
70	1981.0
71	1593.0
72	1412.5
73	1232.0
74	1100.0
75	778.0
76	588.0
77	436.0
78	284.0
79	213.5
80	143.0
81	167.5
82	192.0
83	120.0
84	48.0
85	29.5
86	11.0
87	9.5
88	8.0
89	5.0
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	554709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.006121540282763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70284231910051	20.727618044565205
2	6.689360996690295	3.479286702135097
3	2.1912773064756053	1.7095987188200406
4	1.161254680234922	1.207989214136463
5	0.7656164570684819	0.9955357317881811
6	0.5710635826285514	0.8910689362240449
7	0.44261246041162655	0.8057443408495848
8	0.36363869690937073	0.7565465718860114
9	0.30714690150547574	0.7188929686145394
>10	6.564215399257521	45.76864214862374
>50	1.171331962920909	18.562863965817353
>100	0.06197892074908496	2.4242667861463745
>500	0.005571138943737974	1.1478412438813477
>1k	0.0020891771039017403	0.8041046265120202
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2087	0.37623330430910623	No Hit
GAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT	1272	0.22930942169678156	No Hit
AAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT	1081	0.19487695350174594	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT	985	0.17757058205293225	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	938	0.16909767103111722	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	889	0.16026421060411855	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	786	0.1416959162371622	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT	734	0.1323216317023881	No Hit
TAGAGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT	725	0.13069915937906182	No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	709	0.12781476413759285	No Hit
GCTATGGGGATTCGGATTTTTTTTAAAGGTGCTTGCTTGTCAATGTAAATA	572	0.10311712988251498	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.802747025918094E-4	0.0	0.0	0.1725228903803616	0.0
2	1.802747025918094E-4	0.0	0.0	0.6078862971395813	0.0
3	1.802747025918094E-4	0.0	0.0	0.8858698885361513	0.0
4	1.802747025918094E-4	0.0	0.0	1.6606905602757482	0.0
5	1.802747025918094E-4	0.0	0.0	2.391343929880352	0.0
6	1.802747025918094E-4	0.0	0.0	3.6294705872808986	0.0
7	1.802747025918094E-4	0.0	0.0	4.0534766877768345	0.0
8	1.802747025918094E-4	0.0	0.0	5.3103519142469295	0.0
9	1.802747025918094E-4	0.0	0.0	5.7419295522517215	0.0
10	1.802747025918094E-4	0.0	0.0	6.444460068252003	0.0
11	1.802747025918094E-4	0.0	0.0	7.344391383590315	0.0
12	1.802747025918094E-4	0.0	0.0	7.999329378106358	0.0
13	1.802747025918094E-4	0.0	0.0	8.29443906624915	0.0
14	1.802747025918094E-4	0.0	0.0	8.407831854179399	0.0
15	1.802747025918094E-4	0.0	0.0	8.57638870110274	0.0
16	1.802747025918094E-4	0.0	0.0	8.947033489631501	0.0
17	1.802747025918094E-4	0.0	0.0	9.334263550798708	0.0
18	1.802747025918094E-4	0.0	0.0	9.87959452613893	0.0
19	1.802747025918094E-4	0.0	0.0	10.110346145456447	0.0
20	1.802747025918094E-4	0.0	0.0	10.351012873416511	0.0
21	1.802747025918094E-4	0.0	0.0	10.65874179074073	0.0
22	1.802747025918094E-4	0.0	0.0	10.976205542004907	0.0
23	1.802747025918094E-4	0.0	0.0	11.332248079623731	0.0
24	1.802747025918094E-4	0.0	0.0	11.577782224553776	0.0
25	1.802747025918094E-4	0.0	0.0	11.769053684003685	0.0
26	1.802747025918094E-4	0.0	0.0	11.968978329178002	0.0
27	1.802747025918094E-4	0.0	0.0	12.160249788627912	0.0
28	1.802747025918094E-4	0.0	0.0	12.338721744193803	0.0
29	1.802747025918094E-4	0.0	0.0	12.53287759888518	0.0
30	1.802747025918094E-4	0.0	0.0	12.778051194410041	0.0
31	1.802747025918094E-4	0.0	0.0	13.030255503335983	0.0
32	1.802747025918094E-4	0.0	0.0	13.229639324402525	0.0
33	1.802747025918094E-4	0.0	0.0	13.427941497253515	0.0
34	1.802747025918094E-4	0.0	0.0	13.637240426962606	0.0
35	1.802747025918094E-4	0.0	0.0	13.902965338582932	0.0
36	1.802747025918094E-4	0.0	0.0	14.124162398663083	0.0
37	1.802747025918094E-4	0.0	0.0	14.337607646531785	0.0
38	1.802747025918094E-4	0.0	0.0	14.546005202727917	0.0
39	1.802747025918094E-4	0.0	0.0	14.754222484221456	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAG	110	0.0	45.000004	1
AGCATAG	55	1.8189894E-12	45.000004	1
AGTTAAG	45	3.8380676E-10	45.000004	1
CGTCAGG	55	1.8189894E-12	45.000004	2
AACCGAA	25	3.8881873E-5	45.0	37
AATCCGC	20	7.0301275E-4	45.0	19
TCGTTAG	35	1.2104101E-7	45.0	1
AGTCCAA	35	1.2104101E-7	45.0	43
CGAACAT	20	7.0301275E-4	45.0	40
GTCGATG	40	6.8048394E-9	45.0	1
CGAGTAT	25	3.8881873E-5	45.0	33
TTAGACA	20	7.0301275E-4	45.0	11
ATTACGA	20	7.0301275E-4	45.0	31
CCAATCT	20	7.0301275E-4	45.0	31
TAAACGG	50	2.1827873E-11	45.0	2
CACGATC	20	7.0301275E-4	45.0	17
CCGCTAT	20	7.0301275E-4	45.0	22
TCGATAG	20	7.0301275E-4	45.0	1
CCCGTAT	20	7.0301275E-4	45.0	34
CGCTAAG	20	7.0301275E-4	45.0	29
>>END_MODULE