FastQCFastQC Report
Thu 26 May 2016
SRR1547015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547015_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4021400
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336990.8379917441686974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT214500.042.2412571
TCAAGCG47650.038.29485717
CACGACC48300.037.87267327
AGGGCGA61350.037.8117376
GGCGATA22750.037.7802168
GGGCGAT123400.037.724887
TCGTTAG1500.037.51
ACGACCA48600.037.3611128
AACACGT44100.037.1938841
ACACGAC49250.037.05076226
GTTGATC24200.037.0041316
GACACGA49150.036.9888125
CGAGACA49400.036.98380722
GCGAGAC49450.036.90090621
CATATGC23350.036.8094233
GACCGAT30650.036.778149
AGACACG49450.036.76440424
TGGTCAA49750.036.72361814
GTCAAGC49550.036.69021216
ACACGTG44650.036.63493742
Produced by FastQC (version 0.11.5)