##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547015_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4021400
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63055353856866	31.0	31.0	33.0	30.0	34.0
2	32.007157954941064	33.0	31.0	34.0	30.0	34.0
3	32.069099567314865	33.0	31.0	34.0	30.0	34.0
4	35.772068682548365	37.0	35.0	37.0	35.0	37.0
5	35.73943651464664	37.0	35.0	37.0	33.0	37.0
6	35.79182150494852	37.0	35.0	37.0	35.0	37.0
7	36.11614636693689	37.0	35.0	37.0	35.0	37.0
8	36.04087357636644	37.0	35.0	37.0	35.0	37.0
9	37.80614462624956	39.0	38.0	39.0	35.0	39.0
10	37.34992241507933	39.0	37.0	39.0	34.0	39.0
11	37.119554881384595	39.0	37.0	39.0	33.0	39.0
12	36.770314567066194	39.0	35.0	39.0	33.0	39.0
13	36.694502909434526	39.0	35.0	39.0	33.0	39.0
14	37.78485079822947	40.0	36.0	41.0	33.0	41.0
15	37.85796712587656	40.0	36.0	41.0	33.0	41.0
16	37.91577709255483	40.0	36.0	41.0	33.0	41.0
17	37.83139478788482	40.0	36.0	41.0	33.0	41.0
18	37.73055154921172	39.0	36.0	41.0	33.0	41.0
19	37.63760730093997	39.0	36.0	41.0	33.0	41.0
20	37.48541552693092	39.0	35.0	41.0	33.0	41.0
21	37.41827647088079	39.0	35.0	41.0	33.0	41.0
22	37.32267941512906	39.0	35.0	41.0	32.0	41.0
23	37.20174590938479	39.0	35.0	41.0	32.0	41.0
24	37.10314069726961	39.0	35.0	41.0	32.0	41.0
25	36.973113592281294	39.0	35.0	41.0	32.0	41.0
26	36.82289550902671	39.0	35.0	41.0	31.0	41.0
27	36.73489531009101	38.0	35.0	41.0	31.0	41.0
28	36.75324364649127	39.0	35.0	41.0	31.0	41.0
29	36.73117098522903	39.0	35.0	41.0	31.0	41.0
30	36.62503332172875	38.0	35.0	41.0	31.0	41.0
31	36.4855639826926	38.0	35.0	41.0	31.0	41.0
32	36.348005420997666	39.0	35.0	41.0	30.0	41.0
33	36.146740687322826	38.0	35.0	41.0	30.0	41.0
34	35.906753369473314	38.0	35.0	41.0	29.0	41.0
35	35.74795742776148	38.0	35.0	41.0	28.0	41.0
36	35.60064753568409	38.0	35.0	41.0	27.0	41.0
37	35.51089968667628	38.0	35.0	41.0	27.0	41.0
38	35.3594501914756	38.0	35.0	41.0	27.0	41.0
39	35.24162629929875	38.0	35.0	40.0	26.0	41.0
40	35.07657283533098	38.0	34.0	40.0	25.0	41.0
41	34.9844628736261	38.0	34.0	40.0	25.0	41.0
42	34.87688939175411	37.0	34.0	40.0	24.0	41.0
43	34.739384294026955	37.0	34.0	40.0	24.0	41.0
44	34.5139399214204	37.0	34.0	40.0	23.0	41.0
45	34.41277714228875	36.0	34.0	40.0	23.0	41.0
46	34.367868155368775	36.0	34.0	40.0	23.0	41.0
47	34.309285820858406	36.0	34.0	40.0	23.0	41.0
48	34.20732804495947	36.0	34.0	40.0	23.0	41.0
49	34.108595016660864	36.0	33.0	40.0	23.0	41.0
50	33.99089545929279	35.0	33.0	40.0	23.0	41.0
51	33.63270627144775	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	1.0
11	7.0
12	4.0
13	20.0
14	46.0
15	108.0
16	325.0
17	866.0
18	1776.0
19	3372.0
20	5831.0
21	9384.0
22	14006.0
23	20913.0
24	31193.0
25	46999.0
26	64040.0
27	74577.0
28	78057.0
29	82219.0
30	91330.0
31	106382.0
32	129535.0
33	166899.0
34	279320.0
35	396851.0
36	327400.0
37	427261.0
38	606901.0
39	1055008.0
40	765.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.09382304670015	23.46889143084498	25.577957925100712	14.859327597354156
2	35.23509225642811	24.35519968170289	26.406276421146863	14.003431640722136
3	30.725319540458546	25.275700004973395	30.529517083602727	13.469463370965334
4	26.021112050529666	28.167951459690656	30.3830258119063	15.427910677873378
5	23.796463918038494	31.606181926692194	29.138036504699855	15.459317650569455
6	22.547296961257274	40.12416074004078	26.83816581290098	10.490376485800965
7	86.0224051325409	3.4498433381409463	8.763564927637141	1.7641866016810066
8	85.39429054558114	3.160242701546725	8.660441637240762	2.785025115631372
9	80.12366340080568	4.817476500721142	11.302034117471527	3.7568259810016413
10	42.3744218431392	26.12841299050082	17.18560700253643	14.311558163823543
11	33.351370169592684	26.745088775053468	23.48953100910131	16.41401004625255
12	27.487168647734624	23.32118167802258	29.011612871139402	20.180036803103395
13	24.16357487442184	25.67628189187845	31.572437459591185	18.58770577410852
14	22.51046899089869	26.321057343213806	32.050380464514845	19.11809320137266
15	21.87536678768588	22.768712388720346	35.891555179788135	19.46436564380564
16	25.290968319490727	21.556149599641916	33.18351817774904	19.969363903118317
17	24.290744516834934	22.440319291788928	29.752698065350376	23.516238126025762
18	26.035286218729798	21.866663351071765	30.28641766548963	21.811632764708808
19	27.90125329487243	24.788307554582982	26.626324165713434	20.684114984831155
20	29.993758392599588	23.772218630327746	25.779280847466058	20.454742129606604
21	27.939871686477346	24.907470035311086	27.4862485701497	19.66640970806187
22	26.87243248619884	21.99955239468842	26.824712786591736	24.303302332521014
23	24.887079126672305	26.329561844133885	26.513179489729943	22.270179539463868
24	24.311607897747052	24.32038593524643	30.577385984980353	20.79062018202616
25	25.286442532451385	23.94253245138509	28.660889242552344	22.11013577361118
26	23.214626746904063	26.138732779628988	27.680534142338487	22.966106331128465
27	23.734371114537225	24.318844183617646	28.075918834236834	23.870865867608295
28	22.4808773064107	26.582483712140046	29.129357935047494	21.807281046401748
29	23.84938081265231	24.660242701546725	28.348634803799673	23.141741682001292
30	24.851991843636544	24.964291042920376	28.968966031730243	21.214751081712837
31	26.347167653056147	25.81411946088427	26.15661212513055	21.68210076092903
32	27.112274332322077	24.871910280001988	26.29452429502163	21.721291092654297
33	25.798726811558165	25.61304520813647	25.61816780225792	22.970060178047447
34	23.08720843487343	27.733127766449496	27.915377729149053	21.264286069528023
35	23.741209529019745	27.359750335703986	27.11073258069329	21.788307554582982
36	26.150867856965238	28.91353757397921	24.495747749539962	20.43984681951559
37	25.29370368528373	27.34328840702243	25.074004078181726	22.28900382951211
38	25.266175958621375	28.15492117173124	25.098796439051075	21.48010643059631
39	25.449843338140948	26.269259461878946	24.302929328094695	23.977967871885415
40	26.933058138956582	25.241806336101856	25.91863530113891	21.906500223802656
41	22.970756452976577	25.445491619833888	26.40953399313672	25.174217934052816
42	24.889391754115483	25.66501715820361	25.69498184711792	23.75060924056299
43	24.329561844133885	24.17255184761526	27.707191525339436	23.790694782911423
44	24.482717461580545	25.5237230815139	26.91706868254837	23.07649077435719
45	23.204356691699406	25.318197642612027	26.66161038444323	24.815835281245338
46	24.210175560749988	25.41838663152136	26.742229074451686	23.62920873327697
47	23.558412493161583	25.895459292783606	27.90550554533247	22.640622668722337
48	24.268065847714727	25.26493261053365	27.714079673745463	22.75292186800617
49	24.72798030536629	24.72004774456657	27.437384990301883	23.11458695976526
50	23.13888198139951	25.933182473765353	27.890411299547424	23.03752424528771
51	23.55234495449346	25.50280996667827	26.833814094593922	24.111030984234347
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2200.0
1	3873.0
2	5546.0
3	30806.0
4	56066.0
5	39292.0
6	22518.0
7	22399.0
8	22280.0
9	23265.0
10	24250.0
11	24098.0
12	23946.0
13	23876.5
14	23807.0
15	23215.5
16	22624.0
17	21659.5
18	20695.0
19	20469.5
20	20244.0
21	19809.0
22	19374.0
23	19811.5
24	20249.0
25	22036.0
26	25818.5
27	27814.0
28	30880.0
29	33946.0
30	39126.5
31	44307.0
32	51904.0
33	59501.0
34	67326.5
35	75152.0
36	84621.5
37	94091.0
38	104530.5
39	114970.0
40	132772.5
41	150575.0
42	174390.5
43	198206.0
44	225856.0
45	253506.0
46	274671.5
47	295837.0
48	290919.5
49	286002.0
50	280055.0
51	274108.0
52	266588.0
53	259068.0
54	249946.5
55	240825.0
56	228984.0
57	217143.0
58	214023.0
59	210903.0
60	200578.5
61	190254.0
62	178253.0
63	166252.0
64	152467.5
65	138683.0
66	124538.0
67	110393.0
68	97118.5
69	83844.0
70	73516.0
71	63188.0
72	54360.5
73	45533.0
74	39937.5
75	29172.5
76	24003.0
77	17728.5
78	11454.0
79	8438.0
80	5422.0
81	4078.5
82	2735.0
83	1824.5
84	914.0
85	644.0
86	374.0
87	339.5
88	305.0
89	188.0
90	71.0
91	51.0
92	31.0
93	26.0
94	21.0
95	11.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4021400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.919505389117887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.92077201754704	17.079847821628487
2	6.942636083323187	3.043582980861738
3	2.4658242741441416	1.6214894539716065
4	1.3040151367117532	1.143334672665783
5	0.8716888794891492	0.955349454579827
6	0.6803529332355818	0.8947799871935697
7	0.5494912311866179	0.8431203202287667
8	0.44897000242615315	0.7872960310185868
9	0.39786998184016853	0.7849001890002429
>10	6.22847855807352	34.8163582722149
>50	1.9868390428915028	29.423067959976795
>100	0.19603734592006572	6.206799078529667
>500	0.0047213941255178984	0.7375600079625909
>1k	0.0021879631313281365	0.8118965659151594
>5k	0.0	0.0
>10k+	1.1515595428042823E-4	0.8506172042522914
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33699	0.8379917441686974	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.973392350922564E-5	0.0	0.0	0.06002884567563535	0.0
2	4.973392350922564E-5	0.0	0.0	0.17695329984582484	0.0
3	4.973392350922564E-5	0.0	0.0	0.27933058138956585	0.0
4	4.973392350922564E-5	0.0	0.0	0.48532849256477845	0.0
5	4.973392350922564E-5	0.0	0.0	0.831277664494952	0.0
6	4.973392350922564E-5	0.0	0.0	1.627741582533446	0.0
7	4.973392350922564E-5	0.0	0.0	2.0042771174217933	0.0
8	4.973392350922564E-5	0.0	0.0	2.9680956880688316	0.0
9	4.973392350922564E-5	0.0	0.0	3.5106181926692197	0.0
10	4.973392350922564E-5	0.0	0.0	4.287089073457005	0.0
11	4.973392350922564E-5	0.0	0.0	4.815984483015865	0.0
12	4.973392350922564E-5	0.0	0.0	5.238200626647436	0.0
13	4.973392350922564E-5	0.0	0.0	5.480504301984384	0.0
14	4.973392350922564E-5	0.0	0.0	5.619610086039688	0.0
15	7.460088526383847E-5	0.0	0.0	5.738349828417964	0.0
16	9.946784701845128E-5	0.0	0.0	5.939051076739444	0.0
17	9.946784701845128E-5	0.0	0.0	6.146590739543442	0.0
18	9.946784701845128E-5	0.0	0.0	6.42582185308599	0.0
19	9.946784701845128E-5	0.0	0.0	6.580693290893719	0.0
20	9.946784701845128E-5	0.0	0.0	6.74280101457204	0.0
21	9.946784701845128E-5	0.0	0.0	6.93547023424678	0.0
22	9.946784701845128E-5	0.0	0.0	7.1379867707763465	0.0
23	9.946784701845128E-5	0.0	0.0	7.369523051673546	0.0
24	9.946784701845128E-5	0.0	0.0	7.558387626199831	0.0
25	9.946784701845128E-5	0.0	0.0	7.730690804197543	0.0
26	1.243348087730641E-4	0.0	0.0	7.888024071218979	0.0
27	1.243348087730641E-4	0.0	0.0	8.05515492117173	0.0
28	1.243348087730641E-4	0.0	0.0	8.221514895310092	0.0
29	1.243348087730641E-4	0.0	0.0	8.408539314666534	0.0
30	1.243348087730641E-4	0.0	0.0	8.684786392798529	0.0
31	1.7406873228228975E-4	0.0	0.0	8.889565822847764	0.0
32	1.7406873228228975E-4	0.0	0.0	9.132242502611032	0.0
33	1.7406873228228975E-4	0.0	0.0	9.341746655393644	0.0
34	1.7406873228228975E-4	0.0	0.0	9.550554533247128	0.0
35	1.7406873228228975E-4	0.0	0.0	9.782936290843985	0.0
36	1.7406873228228975E-4	0.0	0.0	9.987094046849355	0.0
37	2.2380265579151538E-4	0.0	0.0	10.19266921967474	0.0
38	2.2380265579151538E-4	0.0	0.0	10.445242950216343	0.0
39	2.2380265579151538E-4	0.0	0.0	10.804222410105933	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	21450	0.0	42.241257	1
TCAAGCG	4765	0.0	38.294857	17
CACGACC	4830	0.0	37.872673	27
AGGGCGA	6135	0.0	37.811737	6
GGCGATA	2275	0.0	37.780216	8
GGGCGAT	12340	0.0	37.72488	7
TCGTTAG	150	0.0	37.5	1
ACGACCA	4860	0.0	37.36111	28
AACACGT	4410	0.0	37.19388	41
ACACGAC	4925	0.0	37.050762	26
GTTGATC	2420	0.0	37.00413	16
GACACGA	4915	0.0	36.98881	25
CGAGACA	4940	0.0	36.983807	22
GCGAGAC	4945	0.0	36.900906	21
CATATGC	2335	0.0	36.80942	33
GACCGAT	3065	0.0	36.77814	9
AGACACG	4945	0.0	36.764404	24
TGGTCAA	4975	0.0	36.723618	14
GTCAAGC	4955	0.0	36.690212	16
ACACGTG	4465	0.0	36.634937	42
>>END_MODULE