FastQCFastQC Report
Thu 26 May 2016
SRR1547013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547013_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1323275
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60090.4541006215639228No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT21220.16035971358938997No Hit
TAGAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT15140.11441310385218492No Hit
GAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT15090.11403525344316186No Hit
AAGAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT14160.1070072358353328No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA207.0336735E-445.00000412
CGTACCG207.0336735E-445.00000437
CGATCGA207.0336735E-445.00000410
GTATCGT253.891127E-545.031
TTAACGG502.1827873E-1145.02
ATACGAG950.042.631581
GCGATAA1900.041.447379
CGTTTTT42700.041.2060851
AATACGG556.184564E-1140.9090922
CGTAAGG2150.040.8139572
AACACGT7600.039.96710641
ACACGAC7750.039.4838726
GACACGA7750.039.4838725
CGGGTAT800.039.3750046
TACGTAG800.039.3750041
ACACGTG7850.038.98089242
CGTATGG1450.038.7931022
ACGATTC356.249007E-638.57142626
TACGATT356.249007E-638.57142625
AGACACG8250.038.45454424