##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547013_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1323275
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.598440233511553	31.0	31.0	33.0	30.0	34.0
2	31.983316393039996	31.0	31.0	34.0	30.0	34.0
3	32.091550131303016	33.0	31.0	34.0	30.0	34.0
4	35.76334246471822	37.0	35.0	37.0	35.0	37.0
5	35.72244393644556	37.0	35.0	37.0	33.0	37.0
6	35.77102643063611	37.0	35.0	37.0	33.0	37.0
7	36.09463792484556	37.0	35.0	37.0	35.0	37.0
8	36.023010334208685	37.0	35.0	37.0	35.0	37.0
9	37.788793712569195	39.0	38.0	39.0	35.0	39.0
10	37.27366496004232	39.0	37.0	39.0	34.0	39.0
11	37.06063365513593	39.0	37.0	39.0	33.0	39.0
12	36.57159093914719	39.0	35.0	39.0	33.0	39.0
13	36.39737771816138	39.0	35.0	39.0	32.0	39.0
14	37.45320889459863	40.0	35.0	41.0	32.0	41.0
15	37.600103531012074	40.0	35.0	41.0	33.0	41.0
16	37.687359014566134	40.0	35.0	41.0	33.0	41.0
17	37.62981542007519	40.0	35.0	41.0	33.0	41.0
18	37.52637282499858	39.0	36.0	41.0	33.0	41.0
19	37.48927320473825	39.0	35.0	41.0	33.0	41.0
20	37.357333131813114	39.0	35.0	41.0	32.0	41.0
21	37.270409400918176	39.0	35.0	41.0	32.0	41.0
22	37.19212181897187	39.0	35.0	41.0	32.0	41.0
23	37.091094443709736	39.0	35.0	41.0	32.0	41.0
24	36.97411951408437	39.0	35.0	41.0	32.0	41.0
25	36.83957756324271	38.0	35.0	41.0	32.0	41.0
26	36.70126315391737	38.0	35.0	41.0	31.0	41.0
27	36.62800627231679	38.0	35.0	41.0	31.0	41.0
28	36.642845213579946	38.0	35.0	41.0	31.0	41.0
29	36.648115471085	38.0	35.0	41.0	31.0	41.0
30	36.556139124520605	38.0	35.0	41.0	31.0	41.0
31	36.42190398821107	38.0	35.0	41.0	31.0	41.0
32	36.31323572197767	38.0	35.0	41.0	30.0	41.0
33	36.19315410628932	38.0	35.0	41.0	30.0	41.0
34	36.0188441555988	38.0	35.0	41.0	30.0	41.0
35	35.91227749334038	38.0	35.0	41.0	30.0	41.0
36	35.78938618201054	38.0	35.0	41.0	29.0	41.0
37	35.70098203321305	38.0	35.0	41.0	29.0	41.0
38	35.5941999962215	38.0	35.0	41.0	29.0	41.0
39	35.48263512875253	38.0	35.0	40.0	28.0	41.0
40	35.32489013999358	38.0	35.0	40.0	27.0	41.0
41	35.23760216130434	38.0	34.0	40.0	27.0	41.0
42	35.132942132209855	37.0	34.0	40.0	27.0	41.0
43	35.004970999981104	37.0	34.0	40.0	26.0	41.0
44	34.79250647068825	37.0	34.0	40.0	25.0	41.0
45	34.70841435075854	37.0	34.0	40.0	24.0	41.0
46	34.664901853356255	36.0	34.0	40.0	24.0	41.0
47	34.61842625304642	36.0	34.0	40.0	25.0	41.0
48	34.519478566435545	36.0	34.0	40.0	24.0	41.0
49	34.420428860214244	36.0	34.0	40.0	24.0	41.0
50	34.3008082220249	36.0	34.0	40.0	24.0	41.0
51	33.93442784001814	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	2.0
12	4.0
13	7.0
14	12.0
15	38.0
16	109.0
17	272.0
18	589.0
19	1244.0
20	1933.0
21	3249.0
22	4684.0
23	7252.0
24	10317.0
25	14358.0
26	17905.0
27	20809.0
28	23228.0
29	25794.0
30	29493.0
31	35091.0
32	43397.0
33	56858.0
34	98462.0
35	160664.0
36	98372.0
37	126979.0
38	185025.0
39	356866.0
40	256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.25850635733313	24.318376754642838	27.11469649165895	13.308420396365078
2	32.30620997147229	26.036235854225314	27.633258393002208	14.024295781300184
3	30.946628629725492	25.630046664525512	29.496589900058567	13.926734805690428
4	24.877217509587954	30.12873363435416	29.17723073435227	15.816818121705618
5	24.24008614989326	33.121913434471296	27.97778239594944	14.660218019686006
6	22.43668171770796	41.867336721392	24.997978500311728	10.698003060588313
7	87.6053730328163	3.7721562033590903	6.878691126183144	1.7437796376414578
8	87.10744176380571	3.4227201450945572	6.271938939373902	3.197899151725832
9	81.39283217774084	5.082616991932894	9.924769983563507	3.5997808467627666
10	44.46498271334379	22.964841019440403	19.2235929795394	13.346583287676408
11	36.97364493397064	25.36653378927283	22.02928340669929	15.630537870057246
12	31.269086168785776	23.613232321323988	25.9099582475298	19.20772326236043
13	23.249362377434775	30.723545748238273	28.935028622168485	17.09206325215847
14	19.94778107347301	32.50435472596399	29.442028300995638	18.10583589956736
15	19.48521660274697	24.053503617917666	38.50152084789632	17.959758931439044
16	21.935123084770737	21.67448187262663	36.845100224820996	19.54529481778164
17	22.11694470159264	22.808410950104854	28.142374034119893	26.932270314182617
18	23.469875876140637	23.22918516559294	31.538644650582835	21.76229430768359
19	27.156033326406074	26.32302431467382	25.788063705578963	20.732878653341142
20	28.715875384935103	25.23164119325159	25.164232680281877	20.88825074153143
21	25.354971566756724	27.938108103002023	26.857304793032437	19.84961553720882
22	25.30925166726493	24.18756494303905	26.14430107120591	24.35888231849011
23	22.965672290340255	28.28074285390414	25.18781054580492	23.56577430995069
24	22.388694715762032	25.53414823071546	32.07806389450417	19.999093159018344
25	22.25750505374922	25.613874667019328	30.012355708375054	22.116264570856398
26	21.14057924467703	29.2776633730706	27.203491337779372	22.378266044472994
27	22.149137556441406	27.664393266705712	27.34034875592753	22.846120420925356
28	20.354877104154465	27.975137443086282	31.076835880674842	20.593149572084414
29	20.98362018476885	25.612363265383237	29.946836447450455	23.45718010239746
30	22.624322230828813	27.357049744006346	28.54894107422871	21.469686950936122
31	27.052502314333754	26.196746707978313	24.52974627345034	22.221004704237593
32	26.64472615291606	26.556233587122858	24.99933876178421	21.799701498176873
33	26.360507075248908	25.646823222686137	24.459012676881223	23.53365702518373
34	21.777257183880902	28.149704332054938	26.60678997185014	23.466248512214012
35	22.068428709074077	26.562052483421812	27.898660520300012	23.470858287204095
36	26.513460920821448	26.837127581190607	25.465077175946043	21.184334322041902
37	22.23717670174378	28.040127713438252	26.511496098694526	23.211199486123444
38	23.00345733124256	28.46974362849748	25.832121063271053	22.69467797698891
39	22.620241446411367	26.811736033704257	26.631123538191233	23.936898981693147
40	24.740737941848824	24.55302185864616	27.379494058302317	23.326746141202698
41	20.581133929077478	24.304396289508983	28.366439326670573	26.748030454742967
42	23.830420736430447	25.806578375621093	26.315845156902384	24.04715573104608
43	22.781281290736995	24.49400162475676	28.363265383234776	24.361451701271466
44	22.67895939997355	26.79329693374393	27.397630877935423	23.130112788347095
45	21.04520980143961	27.8640494228335	26.290982599988666	24.799758175738226
46	23.331129205947367	27.2350796319737	26.69089947289868	22.742891689180254
47	23.01932704842153	25.46719313823657	27.699684494908468	23.81379531843343
48	23.902892444881072	24.243562373656268	28.732274092686705	23.121271088775956
49	23.315712909259222	23.644820615518316	29.36902760197238	23.67043887325008
50	21.92741493642667	26.634373051708828	28.27401711662353	23.164194895240975
51	21.36842304131794	27.97151007915966	26.41831818782944	24.24174869169296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	827.0
1	1389.0
2	1951.0
3	6312.5
4	10674.0
5	7783.5
6	4893.0
7	5070.5
8	5248.0
9	5869.0
10	6490.0
11	6695.5
12	6901.0
13	6933.0
14	6965.0
15	6898.0
16	6831.0
17	6700.5
18	6570.0
19	6552.5
20	6535.0
21	6337.0
22	6139.0
23	6373.0
24	6607.0
25	7815.5
26	10223.5
27	11423.0
28	12173.5
29	12924.0
30	15833.0
31	18742.0
32	20637.5
33	22533.0
34	25653.0
35	28773.0
36	31224.0
37	33675.0
38	37907.0
39	42139.0
40	52184.5
41	62230.0
42	73087.0
43	83944.0
44	88319.0
45	92694.0
46	99308.0
47	105922.0
48	110253.0
49	114584.0
50	110126.5
51	105669.0
52	96433.5
53	87198.0
54	80888.0
55	74578.0
56	68689.0
57	62800.0
58	59362.5
59	55925.0
60	53404.5
61	50884.0
62	47100.5
63	43317.0
64	38289.5
65	33262.0
66	30221.5
67	27181.0
68	23373.5
69	19566.0
70	17394.5
71	15223.0
72	12874.5
73	10526.0
74	9766.0
75	7448.0
76	5890.0
77	4631.5
78	3373.0
79	2639.5
80	1906.0
81	1413.5
82	921.0
83	722.5
84	524.0
85	342.5
86	161.0
87	113.5
88	66.0
89	48.5
90	31.0
91	25.5
92	20.0
93	12.0
94	4.0
95	4.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1323275.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.242551160793358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.47343560201345	19.02396277345034
2	6.465371196849007	3.1347418402626364
3	2.3166866656546707	1.6848718504698341
4	1.3553084713180195	1.3142453981833488
5	0.8999541659488377	1.0908592455191908
6	0.6972290133064787	1.014156601552307
7	0.5556019025435837	0.9428445283212867
8	0.47192680339509735	0.9152567740364252
9	0.425067496900449	0.9274248486359555
>10	6.675361118883362	41.56236988879195
>50	1.5906156687436674	24.60313701761136
>100	0.07030335194585083	2.516249268909716
>500	0.001255416999024366	0.2339653019823923
>1k	0.0015692712487804575	0.5787122233749841
>5k	3.138542497560915E-4	0.4572024388982749
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6009	0.4541006215639228	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	2122	0.16035971358938997	No Hit
TAGAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	1514	0.11441310385218492	No Hit
GAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	1509	0.11403525344316186	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	1416	0.1070072358353328	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.557008180461355E-5	0.0	0.0	0.0696756154238537	0.0
2	7.557008180461355E-5	0.0	0.0	0.21235192987096407	0.0
3	7.557008180461355E-5	0.0	0.0	0.3220041185694584	0.0
4	7.557008180461355E-5	0.0	0.0	0.685193931722431	0.0
5	7.557008180461355E-5	0.0	0.0	1.1043056054108178	0.0
6	7.557008180461355E-5	0.0	0.0	2.1610776293665337	0.0
7	7.557008180461355E-5	0.0	0.0	2.579509172318679	0.0
8	7.557008180461355E-5	0.0	0.0	3.7752545767130794	0.0
9	7.557008180461355E-5	0.0	0.0	4.326991743968563	0.0
10	7.557008180461355E-5	0.0	0.0	5.129319302488145	0.0
11	7.557008180461355E-5	0.0	0.0	5.744686478623113	0.0
12	7.557008180461355E-5	0.0	0.0	6.243675728778976	0.0
13	7.557008180461355E-5	0.0	0.0	6.514745612212125	0.0
14	7.557008180461355E-5	0.0	0.0	6.633995201299806	0.0
15	7.557008180461355E-5	0.0	0.0	6.75233794940583	0.0
16	1.511401636092271E-4	0.0	0.0	6.973984999338762	0.0
17	1.511401636092271E-4	0.0	0.0	7.199410553361924	0.0
18	1.511401636092271E-4	0.0	0.0	7.506451795734069	0.0
19	1.511401636092271E-4	0.0	0.0	7.654115735580284	0.0
20	1.511401636092271E-4	0.0	0.0	7.827171222912849	0.0
21	1.511401636092271E-4	0.0	0.0	8.0262228183862	0.0
22	1.511401636092271E-4	0.0	0.0	8.234040543348888	0.0
23	1.511401636092271E-4	0.0	0.0	8.465587273998224	0.0
24	1.511401636092271E-4	0.0	0.0	8.65602388014585	0.0
25	1.511401636092271E-4	0.0	0.0	8.820539948234494	0.0
26	1.511401636092271E-4	0.0	0.0	8.970773270862066	0.0
27	1.511401636092271E-4	0.0	0.0	9.128639171751903	0.0
28	1.511401636092271E-4	0.0	0.0	9.276454251761727	0.0
29	1.511401636092271E-4	0.0	0.0	9.449736449339706	0.0
30	1.511401636092271E-4	0.0	0.0	9.682416731216112	0.0
31	1.511401636092271E-4	0.0	0.0	9.868167992291852	0.0
32	1.511401636092271E-4	0.0	0.0	10.046059964859912	0.0
33	1.511401636092271E-4	0.0	0.0	10.234305038635204	0.0
34	1.511401636092271E-4	0.0	0.0	10.418469327993048	0.0
35	1.511401636092271E-4	0.0	0.0	10.62704275377378	0.0
36	1.511401636092271E-4	0.0	0.0	10.817932780412235	0.0
37	1.511401636092271E-4	0.0	0.0	11.012072320568286	0.0
38	1.511401636092271E-4	0.0	0.0	11.209839224650961	0.0
39	1.511401636092271E-4	0.0	0.0	11.490053087982467	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGATA	20	7.0336735E-4	45.000004	12
CGTACCG	20	7.0336735E-4	45.000004	37
CGATCGA	20	7.0336735E-4	45.000004	10
GTATCGT	25	3.891127E-5	45.0	31
TTAACGG	50	2.1827873E-11	45.0	2
ATACGAG	95	0.0	42.63158	1
GCGATAA	190	0.0	41.44737	9
CGTTTTT	4270	0.0	41.206085	1
AATACGG	55	6.184564E-11	40.909092	2
CGTAAGG	215	0.0	40.813957	2
AACACGT	760	0.0	39.967106	41
ACACGAC	775	0.0	39.48387	26
GACACGA	775	0.0	39.48387	25
CGGGTAT	80	0.0	39.375004	6
TACGTAG	80	0.0	39.375004	1
ACACGTG	785	0.0	38.980892	42
CGTATGG	145	0.0	38.793102	2
ACGATTC	35	6.249007E-6	38.571426	26
TACGATT	35	6.249007E-6	38.571426	25
AGACACG	825	0.0	38.454544	24
>>END_MODULE