FastQCFastQC Report
Thu 26 May 2016
SRR1547012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547012_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1404706
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63690.4534044846394904No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT20340.14479898284765638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG302.165847E-645.0000041
ATCGATC253.891249E-545.00000416
TAGCATA8150.041.4110430
CGTTTTT44250.041.389831
GCGATAA1150.041.086969
CTATGCG556.184564E-1140.9090921
AGGGTAC8900.040.4494366
CGAGACA11100.040.33783722
ACGACCA11000.040.29545628
CACGACC11250.039.827
CGTAAGG2550.039.7058832
CACGTGA10800.039.58333643
ACACGAC11350.039.44933726
TCGTAAG800.039.3751
TAAGACG403.458954E-739.3751
GTTGATC8900.039.1853916
AACACGT10750.039.1395341
GTAGCAT8800.039.1193229
CGACCAA11350.038.85462629
GACACGA11500.038.73913225
Produced by FastQC (version 0.11.5)