##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547011_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1532613
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.562037513710244	31.0	31.0	33.0	30.0	34.0
2	31.938047634986784	31.0	31.0	34.0	30.0	34.0
3	31.993817747859374	33.0	31.0	34.0	30.0	34.0
4	35.714735552941285	37.0	35.0	37.0	33.0	37.0
5	35.671676411461995	37.0	35.0	37.0	33.0	37.0
6	35.73172875344265	37.0	35.0	37.0	33.0	37.0
7	36.077731951901754	37.0	35.0	37.0	35.0	37.0
8	36.00754985113659	37.0	35.0	37.0	35.0	37.0
9	37.76808561587302	39.0	38.0	39.0	35.0	39.0
10	37.295240872940525	39.0	37.0	39.0	34.0	39.0
11	37.03865359356863	39.0	37.0	39.0	33.0	39.0
12	36.65088642729769	39.0	35.0	39.0	33.0	39.0
13	36.52825794900605	39.0	35.0	39.0	32.0	39.0
14	37.64480465714437	40.0	36.0	41.0	33.0	41.0
15	37.74619620217237	40.0	36.0	41.0	33.0	41.0
16	37.817380512888775	40.0	36.0	41.0	33.0	41.0
17	37.75083729552079	40.0	36.0	41.0	33.0	41.0
18	37.65015760664956	39.0	36.0	41.0	33.0	41.0
19	37.561469203249615	39.0	36.0	41.0	33.0	41.0
20	37.3884418310428	39.0	35.0	41.0	33.0	41.0
21	37.31853703446336	39.0	35.0	41.0	32.0	41.0
22	37.229929538637606	39.0	35.0	41.0	32.0	41.0
23	37.12306564018444	39.0	35.0	41.0	32.0	41.0
24	37.01218311471976	39.0	35.0	41.0	32.0	41.0
25	36.88475107545088	38.0	35.0	41.0	32.0	41.0
26	36.725847294783485	38.0	35.0	41.0	31.0	41.0
27	36.632109345281556	38.0	35.0	41.0	31.0	41.0
28	36.63833922849408	38.0	35.0	41.0	31.0	41.0
29	36.6135841207141	38.0	35.0	41.0	31.0	41.0
30	36.515109163239515	38.0	35.0	41.0	31.0	41.0
31	36.3519910114295	38.0	35.0	41.0	30.0	41.0
32	36.20359347075876	38.0	35.0	41.0	30.0	41.0
33	35.96539830994517	38.0	35.0	41.0	30.0	41.0
34	35.73868484738156	38.0	35.0	41.0	28.0	41.0
35	35.57736297421462	38.0	35.0	41.0	27.0	41.0
36	35.431194959197136	38.0	35.0	41.0	27.0	41.0
37	35.335033697352166	38.0	35.0	41.0	26.0	41.0
38	35.213645584371264	38.0	35.0	41.0	26.0	41.0
39	35.10736239350704	38.0	34.0	40.0	25.0	41.0
40	34.95148742702822	38.0	34.0	40.0	24.0	41.0
41	34.86419533176347	38.0	34.0	40.0	24.0	41.0
42	34.75341459324696	37.0	34.0	40.0	23.0	41.0
43	34.6131926324519	37.0	34.0	40.0	23.0	41.0
44	34.46954449688212	37.0	34.0	40.0	23.0	41.0
45	34.352591293431544	37.0	34.0	40.0	23.0	41.0
46	34.27574345252194	36.0	34.0	40.0	23.0	41.0
47	34.202360282732826	36.0	34.0	40.0	23.0	41.0
48	34.083660389152385	36.0	33.0	40.0	23.0	41.0
49	33.96456704986843	36.0	33.0	40.0	22.0	41.0
50	33.83684400432464	36.0	33.0	40.0	22.0	41.0
51	33.45754733908691	35.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	2.0
13	8.0
14	19.0
15	47.0
16	107.0
17	338.0
18	730.0
19	1473.0
20	2569.0
21	4044.0
22	6069.0
23	9152.0
24	13566.0
25	19828.0
26	25926.0
27	28458.0
28	29431.0
29	31672.0
30	35469.0
31	41659.0
32	50029.0
33	64345.0
34	106915.0
35	169518.0
36	118174.0
37	150145.0
38	215020.0
39	407580.0
40	317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.531434876253826	24.7324667088169	27.62791389607161	15.108184518857664
2	32.544419236950226	26.312774327243734	27.52058086418424	13.622225571621799
3	29.05410563527779	25.310107639697694	32.196451419895304	13.439335305129212
4	24.75171488170856	29.090122555400484	31.130037393653843	15.028125169237114
5	23.004894255757975	32.838165929690014	30.036545429276668	14.120394385275342
6	21.696801475649757	41.64880501470365	26.37645641789545	10.277937091751147
7	86.46957842586485	3.5169348035022536	8.32108301312856	1.6924037575043405
8	85.89135026259075	3.3425920307344383	7.825589369266736	2.9404683374080736
9	80.1577436704504	5.5279447583962815	10.676928878979886	3.637382692173432
10	42.02554721903051	28.906514560427194	15.85299093769921	13.214947282843092
11	33.051853272809254	25.863867786584088	24.73324968534131	16.351029255265352
12	30.80549362428741	21.72146523616856	28.085889914805627	19.387151224738407
13	23.204488021437896	29.064545322269876	29.28449647758436	18.446470178707866
14	19.970794975639645	30.707947798955114	29.373494809191886	19.947762416213354
15	18.74954734169683	25.059816144062463	37.39137016324408	18.799266350996632
16	20.36110877305621	22.27842253719628	37.175399138595324	20.185069551152182
17	20.647025700551932	22.931751198769685	30.017362504428714	26.40386059624967
18	22.021540989147294	23.72327521690081	31.482703069855205	22.772480724096688
19	25.278331842415536	27.21600299619017	26.328107617513357	21.17755754388094
20	27.90880672420239	24.454314298521545	25.993776641591843	21.64310233568422
21	24.917901649013807	27.367182713444294	27.063714062193128	20.651201575348768
22	24.51532121938154	24.618282632340975	25.46637670436046	25.40001944391702
23	23.076275615566356	28.849096281970727	24.97975679444191	23.094871308021006
24	22.11758610947447	25.386774091045815	32.0114079679606	20.48423183151911
25	22.571581997542758	25.71966961000592	28.861232418099025	22.847515974352298
26	21.521675726357532	29.512799382492517	26.8262111831232	22.13931370802675
27	21.93267315362717	28.119949393617304	26.479222086723787	23.468155366031738
28	20.516333869019775	28.264930546719885	30.18615919348198	21.032576390778363
29	21.381849168707298	24.8551982790176	29.617848732850366	24.145103819424733
30	23.55682745742076	27.054579336075058	28.025926962644842	21.362666243859344
31	24.998548231027662	27.66092940618408	24.482501453400175	22.858020909388085
32	27.700600216754	26.12910108422674	24.44752850197669	21.722770197042568
33	25.18939875885171	27.66203862292699	24.112088309312266	23.036474308909032
34	23.29948917306587	27.045575106044385	26.069138132065955	23.58579758882379
35	22.897691719958008	26.953314372251835	26.081209020150553	24.067784887639608
36	26.251506414208936	27.53806733989598	24.16448248840379	22.04594375749129
37	23.63988821705153	28.924001036138936	24.790667963797773	22.645442783011756
38	22.827158584717733	28.165753520295077	25.511854590819734	23.495233304167456
39	22.81821960273076	28.571009119719072	24.395395315059968	24.215375962490203
40	24.332496200932656	26.349900464109332	26.589491280577683	22.72811205438033
41	20.42981496307287	26.458081720564813	26.863859304338405	26.248244012023907
42	23.716619916443353	27.385974150030044	23.844767074271196	25.052638859255406
43	22.785660828924197	26.718225670798823	26.536183628874348	23.95992987140263
44	23.18615332115805	27.995456126236697	26.0988912399934	22.71949931261186
45	21.724727638353585	28.04021628421526	24.889127261741876	25.345928815689284
46	23.313778494636285	27.848974268129005	25.348603985481006	23.488643251753704
47	23.585667092736394	26.465976733852575	26.459451929482526	23.488904243928506
48	24.054474286724698	24.363880509952608	27.17483148061513	24.40681372270756
49	22.65536048565424	25.511332606470127	27.091574976853256	24.74173193102238
50	21.471565228795527	27.129418842199566	27.901759935482733	23.497255993522174
51	21.388243476989953	27.565993502599806	26.08597212734069	24.95979089306955
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1068.0
1	1519.5
2	1971.0
3	9398.5
4	16826.0
5	12195.0
6	7564.0
7	7854.5
8	8145.0
9	8883.5
10	9622.0
11	10003.5
12	10385.0
13	10557.5
14	10730.0
15	10558.5
16	10387.0
17	10378.5
18	10370.0
19	10173.0
20	9976.0
21	9597.0
22	9218.0
23	9133.0
24	9048.0
25	9483.0
26	10701.5
27	11485.0
28	13077.5
29	14670.0
30	16624.5
31	18579.0
32	20571.5
33	22564.0
34	27535.5
35	32507.0
36	35073.5
37	37640.0
38	41825.0
39	46010.0
40	56032.5
41	66055.0
42	75172.5
43	84290.0
44	91612.0
45	98934.0
46	100981.5
47	103029.0
48	112290.0
49	121551.0
50	124839.5
51	128128.0
52	121562.0
53	114996.0
54	103861.0
55	92726.0
56	85189.5
57	77653.0
58	73854.5
59	70056.0
60	67311.5
61	64567.0
62	57030.5
63	49494.0
64	45687.5
65	41881.0
66	36144.5
67	30408.0
68	26988.0
69	23568.0
70	20762.5
71	17957.0
72	15994.0
73	14031.0
74	12150.5
75	8532.5
76	6795.0
77	5434.0
78	4073.0
79	2991.5
80	1910.0
81	1381.0
82	852.0
83	607.5
84	363.0
85	281.5
86	200.0
87	137.5
88	75.0
89	55.5
90	36.0
91	30.5
92	25.0
93	15.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1532613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.298955383141788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.27374211580673	21.867661804236192
2	7.462679213516231	4.22372052203991
3	2.763433693088409	2.346068603549389
4	1.636739698848495	1.8527209484612197
5	1.1416869390581177	1.6154273874960694
6	0.8406501061543954	1.4273711908137987
7	0.6804553585329125	1.347932308193985
8	0.5773448409382326	1.307060471551851
9	0.4893941449721587	1.246440876600415
>10	6.046011128610401	40.588922833272804
>50	1.02905081737463	18.3389433964105
>100	0.055789985726958945	2.3493176366402055
>500	0.0013947495564607159	0.28885429077351565
>1k	0.0013947495564607159	0.5012684344555202
>5k	0.0	0.0
>10k+	2.324582594101193E-4	0.6982892955046388
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10615	0.6926079838811233	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	1739	0.11346634799522122	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.524804370052975E-5	0.0	0.0	0.07699269156662511	0.0
2	6.524804370052975E-5	0.0	0.0	0.1999852539421237	0.0
3	6.524804370052975E-5	0.0	0.0	0.3285239000321673	0.0
4	6.524804370052975E-5	0.0	0.0	0.586906153086265	0.0
5	6.524804370052975E-5	0.0	0.0	0.9378753801514146	0.0
6	6.524804370052975E-5	0.0	0.0	1.89186702709686	0.0
7	6.524804370052975E-5	0.0	0.0	2.324657300962474	0.0
8	6.524804370052975E-5	0.0	0.0	3.56326091452963	0.0
9	6.524804370052975E-5	0.0	0.0	4.233097331159269	0.0
10	6.524804370052975E-5	0.0	0.0	5.1375004648923115	0.0
11	6.524804370052975E-5	0.0	0.0	5.847399180354075	0.0
12	6.524804370052975E-5	0.0	0.0	6.4271932966769825	0.0
13	6.524804370052975E-5	0.0	0.0	6.795583751410173	0.0
14	6.524804370052975E-5	0.0	0.0	6.988652712720041	0.0
15	6.524804370052975E-5	0.0	0.0	7.155165720243793	0.0
16	6.524804370052975E-5	0.0	0.0	7.404739487398319	0.0
17	6.524804370052975E-5	0.0	0.0	7.666579886768545	0.0
18	6.524804370052975E-5	0.0	0.0	7.999344909641247	0.0
19	6.524804370052975E-5	0.0	0.0	8.208464889701444	0.0
20	6.524804370052975E-5	0.0	0.0	8.426067115442711	0.0
21	6.524804370052975E-5	0.0	0.0	8.674662161941729	0.0
22	6.524804370052975E-5	0.0	0.0	8.950204650489066	0.0
23	6.524804370052975E-5	0.0	0.0	9.2498889152056	0.0
24	6.524804370052975E-5	0.0	0.0	9.4963307762625	0.0
25	6.524804370052975E-5	0.0	0.0	9.722937232034441	0.0
26	6.524804370052975E-5	0.0	0.0	9.932057212094639	0.0
27	6.524804370052975E-5	0.0	0.0	10.131520481687158	0.0
28	6.524804370052975E-5	0.0	0.0	10.340770957834756	0.0
29	6.524804370052975E-5	0.0	0.0	10.561374593586248	0.0
30	1.304960874010595E-4	0.0	0.0	10.871433297251166	0.0
31	1.304960874010595E-4	0.0	0.0	11.107826959578185	0.0
32	1.304960874010595E-4	0.0	0.0	11.350223441925653	0.0
33	1.304960874010595E-4	0.0	0.0	11.586747600340074	0.0
34	1.304960874010595E-4	0.0	0.0	11.822619278317488	0.0
35	1.304960874010595E-4	0.0	0.0	12.080479547021982	0.0
36	1.304960874010595E-4	0.0	0.0	12.33416394092964	0.0
37	1.304960874010595E-4	0.0	0.0	12.583150475690863	0.0
38	1.304960874010595E-4	0.0	0.0	12.844664634842585	0.0
39	1.304960874010595E-4	0.0	0.0	13.094434146128213	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7815	0.0	42.955853	1
TGTAACG	105	0.0	42.857143	1
TACGGGA	370	0.0	40.743244	4
TAATCGT	345	0.0	40.43478	21
CACAACG	295	0.0	40.42373	12
TGCGTAG	135	0.0	40.0	1
CGAAAGG	240	0.0	39.374996	2
TCGTTGA	355	0.0	39.295776	24
TTAATCG	360	0.0	38.75	20
ACGGGAT	410	0.0	38.414635	5
CGTTAGG	215	0.0	37.674416	2
CATACGA	575	0.0	37.565216	18
TCGAGTA	30	1.1400999E-4	37.499996	42
TAGGGAT	1615	0.0	37.33746	5
GCGTTAG	115	0.0	37.173916	1
TAAGGGA	1665	0.0	37.162163	4
AGGGATC	1060	0.0	37.146225	6
ATAGGGA	1825	0.0	37.109592	4
CGTTGAT	370	0.0	37.094593	25
GTTGATC	765	0.0	37.058826	16
>>END_MODULE