FastQCFastQC Report
Thu 26 May 2016
SRR1547010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547010_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3300270
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188680.5717107994194415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG453.8562575E-1045.01
TCGAATA207.0352096E-445.018
CGTTTTT109200.041.5178531
AGGGTAC13500.039.6666686
TCGATAG1200.039.3750041
CTAAGCG800.039.3751
ATAACGG1600.039.3752
TATAGCG2150.038.7209321
GTTGATC19250.038.6883116
TAGCATA18700.038.26203530
ACACGAC21800.038.18807226
AACACGT20050.038.15461341
CATATGC18650.038.12332533
GGGCGAT76850.038.0611577
CACGACC21950.038.0296127
TGGTCAA22100.037.46606414
AGACACG22400.036.96428324
ATAGGGC15100.036.9536444
GGTACCT21900.036.883568
GCCAGTA19850.036.83878725
Produced by FastQC (version 0.11.5)