##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547010_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3300270
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.625427313522835	31.0	31.0	33.0	30.0	34.0
2	32.01523208707167	31.0	31.0	34.0	30.0	34.0
3	32.11339375263236	33.0	31.0	34.0	30.0	34.0
4	35.79478800219376	37.0	35.0	37.0	35.0	37.0
5	35.74161780702791	37.0	35.0	37.0	33.0	37.0
6	35.789341781127	37.0	35.0	37.0	35.0	37.0
7	36.113049235365594	37.0	35.0	37.0	35.0	37.0
8	36.04355764831362	37.0	35.0	37.0	35.0	37.0
9	37.80389331781945	39.0	38.0	39.0	35.0	39.0
10	37.36633669366446	39.0	37.0	39.0	34.0	39.0
11	37.1023601099304	39.0	37.0	39.0	33.0	39.0
12	36.6230326609641	39.0	35.0	39.0	33.0	39.0
13	36.46060079932854	39.0	35.0	39.0	32.0	39.0
14	37.49476133770873	40.0	35.0	41.0	32.0	41.0
15	37.60511352101495	40.0	35.0	41.0	33.0	41.0
16	37.713992188517906	40.0	35.0	41.0	33.0	41.0
17	37.650802510097655	40.0	35.0	41.0	33.0	41.0
18	37.566225187636164	39.0	36.0	41.0	33.0	41.0
19	37.518849366869986	39.0	35.0	41.0	33.0	41.0
20	37.368734376278304	39.0	35.0	41.0	32.0	41.0
21	37.29141130877171	39.0	35.0	41.0	32.0	41.0
22	37.22015471461426	39.0	35.0	41.0	32.0	41.0
23	37.116591369797014	39.0	35.0	41.0	32.0	41.0
24	37.007828147394	39.0	35.0	41.0	32.0	41.0
25	36.87352731746191	38.0	35.0	41.0	31.0	41.0
26	36.72945516578947	38.0	35.0	41.0	31.0	41.0
27	36.62719535068343	38.0	35.0	41.0	31.0	41.0
28	36.67465783102595	38.0	35.0	41.0	31.0	41.0
29	36.65303747875174	38.0	35.0	41.0	31.0	41.0
30	36.56755447281586	38.0	35.0	41.0	31.0	41.0
31	36.43740027331097	38.0	35.0	41.0	31.0	41.0
32	36.350688883030784	38.0	35.0	41.0	30.0	41.0
33	36.21933841776582	38.0	35.0	41.0	30.0	41.0
34	36.02540761816457	38.0	35.0	41.0	30.0	41.0
35	35.90052026046354	38.0	35.0	41.0	29.0	41.0
36	35.79578640535471	38.0	35.0	41.0	29.0	41.0
37	35.720106839743416	38.0	35.0	41.0	29.0	41.0
38	35.59081711496332	38.0	35.0	40.0	29.0	41.0
39	35.49145645659294	38.0	35.0	40.0	28.0	41.0
40	35.308192057013514	37.0	35.0	40.0	27.0	41.0
41	35.20390543803992	37.0	34.0	40.0	27.0	41.0
42	35.119368718316984	37.0	34.0	40.0	27.0	41.0
43	34.98147394001097	37.0	34.0	40.0	26.0	41.0
44	34.82732382502038	37.0	34.0	40.0	26.0	41.0
45	34.71173358543392	36.0	34.0	40.0	26.0	41.0
46	34.641522057286224	36.0	34.0	40.0	26.0	41.0
47	34.561517391001345	36.0	34.0	40.0	25.0	41.0
48	34.46216006569159	36.0	34.0	40.0	25.0	41.0
49	34.38163907801483	36.0	34.0	40.0	25.0	41.0
50	34.25698261051369	35.0	34.0	40.0	24.0	41.0
51	33.89234274771458	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	6.0
12	4.0
13	19.0
14	35.0
15	110.0
16	271.0
17	670.0
18	1407.0
19	2698.0
20	4527.0
21	7288.0
22	11291.0
23	16401.0
24	24174.0
25	33561.0
26	43786.0
27	51039.0
28	56750.0
29	63530.0
30	73960.0
31	88837.0
32	109746.0
33	143472.0
34	251342.0
35	383137.0
36	262823.0
37	342399.0
38	482298.0
39	844032.0
40	652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.75200513897348	23.930799601244747	27.510537016668334	13.806658243113443
2	32.44246682847161	26.396082744745126	27.613922497250226	13.547527929533038
3	30.21322497856236	25.896396355449706	29.953549255060953	13.936829410926984
4	26.03235492853615	29.05522881461214	29.193884136752445	15.718532120099265
5	23.913770691488878	32.38695621873362	28.64986804109967	15.049405048677835
6	22.808770191529785	40.83696182433558	25.514124601926508	10.840143382208122
7	87.31752250573437	3.6809109557702855	7.06639153766207	1.935175000833265
8	86.75878034221442	3.4482936244610287	6.773900317246771	3.0190257160777754
9	81.1776006205553	5.237056362055226	9.676693119047835	3.9086498983416504
10	46.6973308244477	24.632530065721895	15.118975114157326	13.551163995673082
11	37.16350480415239	25.155487278313593	21.113060446569524	16.567947470964498
12	32.25139155281235	20.903986643517044	26.758265232844586	20.086356570826023
13	25.12430801116272	27.436240065206785	28.51303075202938	18.926421171601113
14	21.655410011908117	28.818308805037162	29.963245431434398	19.563035751620323
15	20.535501640774847	22.822072133492107	37.20395603996037	19.43847018577268
16	23.73487623739876	20.571256291151936	34.748005466219425	20.945862005229877
17	23.969826711147878	21.169449772291358	27.885294233502105	26.97542928305866
18	24.91826426322695	21.800610253100505	30.631584688525482	22.649540795147065
19	28.714590018392432	24.80597042060195	24.780608859275148	21.698830701730465
20	30.089083620431055	24.002854311919933	24.502055892396683	21.40600617525233
21	27.631254412517762	25.17472812830465	26.179585306656726	21.014432152520854
22	26.728116184433393	22.49400806600672	25.318504243592194	25.45937150596769
23	24.931505604086937	26.25670020937681	24.91374948110306	23.89804470543319
24	24.168295321291893	23.553769843073447	30.13874622379381	22.139188611840847
25	25.027831056246914	23.198617082844734	28.22572092586364	23.547830935044708
26	22.710141897481115	26.582188730013	26.375296566644547	24.33237280586134
27	22.900096052747198	25.313686455956635	26.96330906259185	24.82290842870432
28	22.056680211013038	26.31099879706812	29.07089419956549	22.561426792353352
29	23.323667457511053	23.60415965966421	28.942692567577804	24.129480315246933
30	24.25868186542313	23.89971123574738	28.98596175464432	22.85564514418517
31	26.878922027591685	24.511691467667795	25.57475600481173	23.03463049992879
32	28.2250240131868	23.51092486372327	25.378347832147067	22.885703290942864
33	26.404566899071895	23.913770691488878	25.97654131328649	23.70512109615274
34	23.241825668808914	25.021043732785497	27.648040917864297	24.08908968054129
35	23.03375178394495	24.77115508731104	29.06671272350444	23.12838040523957
36	27.5695624903417	24.790668642262602	24.786578067855054	22.85319079954064
37	24.49117799452772	25.86988337317856	26.476167101479575	23.162771530814148
38	25.3784084332494	25.783193496289698	25.30111172722232	23.537286343238584
39	24.201383523166285	24.719432046468924	25.11921751856666	25.959966911798126
40	26.82159338478367	22.6499650028634	26.491741584779426	24.036700027573502
41	22.129128828853396	22.7257769818833	27.479236547312798	27.66585764195051
42	24.957412575334743	24.444575746832836	25.216664091119817	25.381347586712604
43	23.43493108139637	22.939759474224836	27.83854048305139	25.786768961327404
44	23.84989712962879	23.942404712341716	27.438785311504816	24.768912846524678
45	22.390137776606156	25.11055156093289	26.12168095337654	26.37762970908441
46	24.493389934762913	24.23853199889706	26.906677332460678	24.36140073387935
47	23.567344489996273	24.524357098055614	27.741063609947066	24.167234802001047
48	23.855139124980685	22.947001305953755	28.276231944659074	24.921627624406486
49	24.024973714271862	22.444799970911472	28.65338290503504	24.876843409781625
50	23.045387195593086	24.659921763976886	27.68973447627012	24.604956564159902
51	22.341050883715575	25.601026582673537	26.290939832195548	25.766982701415337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2039.0
1	3411.5
2	4784.0
3	17991.0
4	31198.0
5	21542.0
6	11886.0
7	12084.0
8	12282.0
9	13190.0
10	14098.0
11	14774.5
12	15451.0
13	15177.0
14	14903.0
15	15085.5
16	15268.0
17	14894.0
18	14520.0
19	14126.5
20	13733.0
21	13954.5
22	14176.0
23	14239.0
24	14302.0
25	15291.5
26	17780.5
27	19280.0
28	21670.5
29	24061.0
30	28032.0
31	32003.0
32	37430.0
33	42857.0
34	51277.0
35	59697.0
36	63417.0
37	67137.0
38	75863.0
39	84589.0
40	103231.0
41	121873.0
42	144023.0
43	166173.0
44	175908.5
45	185644.0
46	207886.5
47	230129.0
48	245996.5
49	261864.0
50	256159.0
51	250454.0
52	237478.0
53	224502.0
54	212615.0
55	200728.0
56	193557.0
57	186386.0
58	185296.5
59	184207.0
60	179015.5
61	173824.0
62	163429.0
63	153034.0
64	139448.0
65	125862.0
66	109176.0
67	92490.0
68	80992.0
69	69494.0
70	60807.0
71	52120.0
72	44661.0
73	37202.0
74	31901.0
75	21719.5
76	16839.0
77	12736.0
78	8633.0
79	6247.5
80	3862.0
81	3107.5
82	2353.0
83	1533.5
84	714.0
85	582.5
86	451.0
87	315.5
88	180.0
89	106.5
90	33.0
91	41.5
92	50.0
93	30.5
94	11.0
95	12.0
96	13.0
97	6.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3300270.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.83117772423133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4096431563177	16.33365211882813
2	7.908861112574611	3.295017828646073
3	2.6196886359289104	1.6371359867155284
4	1.3429374887308587	1.1189987800114172
5	0.8737618455645317	0.9100744146803565
6	0.6163535080577447	0.7703621680382596
7	0.4864715703766071	0.7093643018210707
8	0.4092937836156037	0.6820857238335779
9	0.31610254960083045	0.5926309550835794
>10	4.2873927751039185	21.935533894741603
>50	2.003118404642556	30.530437772984854
>100	0.7193085927213173	19.263380846770918
>500	0.00426939012876897	0.5939113095747749
>1k	0.0026499662868221196	1.0487751195310415
>5k	0.0	0.0
>10k+	1.4722034926789555E-4	0.5786387787388646
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18868	0.5717107994194415	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2120220466810291E-4	0.0	0.0	0.07069118587267102	0.0
2	1.2120220466810291E-4	0.0	0.0	0.24607077602741595	0.0
3	1.2120220466810291E-4	0.0	0.0	0.39021049792895734	0.0
4	1.2120220466810291E-4	0.0	0.0	0.6777021274016974	0.0
5	1.2120220466810291E-4	0.0	0.0	1.1240898471943204	0.0
6	1.2120220466810291E-4	0.0	0.0	2.011138482608999	0.0
7	1.2120220466810291E-4	0.0	0.0	2.4008641717192836	0.0
8	1.2120220466810291E-4	0.0	0.0	3.37748123638369	0.0
9	1.2120220466810291E-4	0.0	0.0	3.8708045099340356	0.0
10	1.2120220466810291E-4	0.0	0.0	4.61292560911683	0.0
11	1.2120220466810291E-4	0.0	0.0	5.2934153872258936	0.0
12	1.2120220466810291E-4	0.0	0.0	5.840976647365215	0.0
13	1.2120220466810291E-4	0.0	0.0	6.128407675735621	0.0
14	1.5150275583512864E-4	0.0	0.0	6.274910840628191	0.0
15	1.5150275583512864E-4	0.0	0.0	6.420080781269411	0.0
16	1.5150275583512864E-4	0.0	0.0	6.673363088474579	0.0
17	1.5150275583512864E-4	0.0	0.0	6.925524275286567	0.0
18	1.5150275583512864E-4	0.0	0.0	7.266859984183112	0.0
19	1.5150275583512864E-4	0.0	0.0	7.457177746063201	0.0
20	1.5150275583512864E-4	0.0	0.0	7.659009717386759	0.0
21	1.5150275583512864E-4	0.0	0.0	7.896232732473404	0.0
22	1.5150275583512864E-4	0.0	0.0	8.140546076533132	0.0
23	1.5150275583512864E-4	0.0	0.0	8.41970505443494	0.0
24	1.5150275583512864E-4	0.0	0.0	8.643383723149924	0.0
25	1.5150275583512864E-4	0.0	0.0	8.837882961091061	0.0
26	1.5150275583512864E-4	0.0	0.0	9.016141103606675	0.0
27	1.8180330700215438E-4	0.0	0.0	9.205761952809922	0.0
28	1.8180330700215438E-4	0.0	0.0	9.386292636663061	0.0
29	1.8180330700215438E-4	0.0	0.0	9.587882203577283	0.0
30	2.1210385816918009E-4	0.0	0.0	9.896069109497102	0.0
31	2.1210385816918009E-4	0.0	0.0	10.135534365370106	0.0
32	2.4240440933620582E-4	0.0	0.0	10.384695797616558	0.0
33	2.4240440933620582E-4	0.0	0.0	10.611465122550578	0.0
34	2.4240440933620582E-4	0.0	0.0	10.836931523784418	0.0
35	2.4240440933620582E-4	0.0	0.0	11.11339375263236	0.0
36	2.4240440933620582E-4	0.0	0.0	11.328285261508906	0.0
37	2.4240440933620582E-4	0.0	0.0	11.56084199171583	0.0
38	2.4240440933620582E-4	0.0	0.0	11.827395940332154	0.0
39	2.4240440933620582E-4	0.0	0.0	12.23393843534014	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	45	3.8562575E-10	45.0	1
TCGAATA	20	7.0352096E-4	45.0	18
CGTTTTT	10920	0.0	41.517853	1
AGGGTAC	1350	0.0	39.666668	6
TCGATAG	120	0.0	39.375004	1
CTAAGCG	80	0.0	39.375	1
ATAACGG	160	0.0	39.375	2
TATAGCG	215	0.0	38.720932	1
GTTGATC	1925	0.0	38.68831	16
TAGCATA	1870	0.0	38.262035	30
ACACGAC	2180	0.0	38.188072	26
AACACGT	2005	0.0	38.154613	41
CATATGC	1865	0.0	38.123325	33
GGGCGAT	7685	0.0	38.061157	7
CACGACC	2195	0.0	38.02961	27
TGGTCAA	2210	0.0	37.466064	14
AGACACG	2240	0.0	36.964283	24
ATAGGGC	1510	0.0	36.953644	4
GGTACCT	2190	0.0	36.88356	8
GCCAGTA	1985	0.0	36.838787	25
>>END_MODULE