FastQCFastQC Report
Thu 26 May 2016
SRR1547009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547009_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1879602
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94500.5027660111023503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG351.2124838E-745.01
CGTTTTT60550.040.9496271
CTCACGA1650.040.9090924
ACACGAC20000.040.72500226
CACGACC20000.040.61250327
AGGGCGA24600.040.152446
GACACGA20450.039.93887325
AGACACG20450.039.8288524
ACGACCA20450.039.8288528
CGAGACA20650.039.55205522
CACGTGA19200.039.2578143
CGACCAA20850.039.17265729
TCAAGCG20750.039.14457717
AACACGT19550.039.13043241
CATACGA3050.039.09836218
AGGGTAC8550.038.947376
TAAACGG1100.038.8636362
CGTAAGG3250.038.769232
GTCAAGC20950.038.66348316
AAACACG20500.038.63414840
Produced by FastQC (version 0.11.5)