##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547009_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1879602
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.595004687162493	31.0	31.0	33.0	30.0	34.0
2	31.967684648132956	31.0	31.0	34.0	30.0	34.0
3	32.07075540460161	33.0	31.0	34.0	30.0	34.0
4	35.75096323583397	37.0	35.0	37.0	35.0	37.0
5	35.697818474336586	37.0	35.0	37.0	33.0	37.0
6	35.747561451839275	37.0	35.0	37.0	33.0	37.0
7	36.09289785816359	37.0	35.0	37.0	35.0	37.0
8	36.02110659597085	37.0	35.0	37.0	35.0	37.0
9	37.79101213980407	39.0	38.0	39.0	35.0	39.0
10	37.3029205118956	39.0	37.0	39.0	34.0	39.0
11	37.06675402558627	39.0	37.0	39.0	33.0	39.0
12	36.61323620638837	39.0	35.0	39.0	33.0	39.0
13	36.47648065920338	39.0	35.0	39.0	32.0	39.0
14	37.54842035707559	40.0	35.0	41.0	32.0	41.0
15	37.636242672650916	40.0	35.0	41.0	33.0	41.0
16	37.71013065531958	40.0	35.0	41.0	33.0	41.0
17	37.630250978664634	40.0	35.0	41.0	33.0	41.0
18	37.56234298537669	39.0	36.0	41.0	33.0	41.0
19	37.50635294067574	39.0	35.0	41.0	33.0	41.0
20	37.370834357486316	39.0	35.0	41.0	32.0	41.0
21	37.29387604397101	39.0	35.0	41.0	32.0	41.0
22	37.211589474793065	39.0	35.0	41.0	32.0	41.0
23	37.11291486176329	39.0	35.0	41.0	32.0	41.0
24	37.010048935891746	39.0	35.0	41.0	32.0	41.0
25	36.88448511972216	39.0	35.0	41.0	31.0	41.0
26	36.731080303170565	38.0	35.0	41.0	31.0	41.0
27	36.64688481923301	38.0	35.0	41.0	31.0	41.0
28	36.66889479794126	38.0	35.0	41.0	31.0	41.0
29	36.66628201076611	38.0	35.0	41.0	31.0	41.0
30	36.56284734747037	38.0	35.0	41.0	31.0	41.0
31	36.441828642446644	38.0	35.0	41.0	31.0	41.0
32	36.32257041650307	38.0	35.0	41.0	30.0	41.0
33	36.17252641782675	38.0	35.0	41.0	30.0	41.0
34	35.99259098468718	38.0	35.0	41.0	30.0	41.0
35	35.879769759768294	38.0	35.0	41.0	29.0	41.0
36	35.7487122273758	38.0	35.0	41.0	29.0	41.0
37	35.68409801649498	38.0	35.0	41.0	28.0	41.0
38	35.549549319483596	38.0	35.0	40.0	28.0	41.0
39	35.45068956087512	38.0	35.0	40.0	28.0	41.0
40	35.304203762285844	38.0	34.0	40.0	27.0	41.0
41	35.209680560033455	37.0	34.0	40.0	27.0	41.0
42	35.11651349594223	37.0	34.0	40.0	27.0	41.0
43	34.981750391838276	37.0	34.0	40.0	26.0	41.0
44	34.82309871983537	37.0	34.0	40.0	26.0	41.0
45	34.6925891757936	36.0	34.0	40.0	25.0	41.0
46	34.62402359648479	36.0	34.0	40.0	25.0	41.0
47	34.530989539274806	36.0	34.0	40.0	25.0	41.0
48	34.411539783422235	36.0	34.0	40.0	25.0	41.0
49	34.29276144630619	36.0	34.0	40.0	24.0	41.0
50	34.1575780404575	35.0	34.0	40.0	24.0	41.0
51	33.78959269036743	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	2.0
11	1.0
12	6.0
13	8.0
14	26.0
15	70.0
16	158.0
17	419.0
18	831.0
19	1679.0
20	2745.0
21	4422.0
22	6448.0
23	9857.0
24	14092.0
25	19837.0
26	25451.0
27	29832.0
28	33205.0
29	37619.0
30	43562.0
31	51936.0
32	63018.0
33	81061.0
34	137561.0
35	212138.0
36	146660.0
37	192065.0
38	278866.0
39	485714.0
40	311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.00980420323025	24.43086355515689	26.729754490578323	12.829577751034527
2	32.44506017763335	25.511198647373217	28.004226426658406	14.03951474833502
3	30.491295497663867	25.644045920359737	29.75768274347442	14.106975838501981
4	25.729063918850905	29.218366441406214	29.214535843226386	15.838033796516497
5	24.29477091426802	32.0000191529909	28.306737277359783	15.398472655381298
6	22.14692259318728	41.52214138950693	25.41107106717273	10.919864950133059
7	87.61615490939039	3.6156590597371148	6.9309353788727615	1.837250651999732
8	87.11094157167315	3.267553450145297	6.588628869303183	3.032876108878369
9	81.67287542788314	4.913327395906155	9.577559504618531	3.8362376715921775
10	43.733194580554816	25.422935280979697	15.529457832030397	15.314412306435084
11	35.31311415927414	25.7669442786292	22.29541147540809	16.624530086688566
12	30.480867758174334	22.226886330191178	27.006462006318362	20.285783905316126
13	24.393781236666058	28.61776056846077	28.76534500388912	18.22311319098405
14	21.49747659344904	29.63755092833483	29.39047734573596	19.474495132480175
15	20.87186542682972	23.76354143057945	35.930851318523814	19.43374182406701
16	23.872394262189548	21.41357585276032	34.35184682714745	20.362183057902683
17	23.10478494915413	22.665330213523927	28.142340772142187	26.087544065179756
18	25.355846610080217	22.574672723268012	30.05455410241104	22.014926564240728
19	27.596054909496797	25.70655915454442	25.147823847814593	21.549562088144192
20	29.658991637591363	24.76737096470423	24.38915259719877	21.18448480050564
21	26.785244961433325	26.568124528490607	26.397822517745777	20.248807992330292
22	26.103770904691526	22.971990878920113	25.65830425802909	25.26593395835927
23	24.164849792668875	27.135957505897522	24.98709833251933	23.71209436891427
24	23.6138288850512	24.7568900224622	30.447935254378322	21.18134583810828
25	24.347335233735652	24.751942166479925	28.750927057962272	22.14979554182215
26	22.31030824610742	27.187723784077694	26.401919129688096	24.100048840126792
27	22.795410943380567	25.830521567863833	26.658143585716548	24.715923903039048
28	21.86638447926742	27.0832335781724	29.138775123669795	21.911606818890384
29	23.268755832351744	24.482204211317075	27.949480794338378	24.299559161992804
30	25.57504195037034	25.54179023005934	27.54359699553416	21.33957082403615
31	27.671549615290896	26.076477892660254	24.178150480793274	22.073822011255576
32	28.73491302946049	25.06344428235339	24.631810351340334	21.569832336845778
33	27.455865656665612	25.843822255988236	23.800357735307795	22.899954352038357
34	24.046846087629188	27.128828337062842	27.061314044143387	21.763011531164576
35	23.972894261657522	26.752950890667282	27.806737809387304	21.46741703828789
36	27.593926799396897	28.295777510345278	24.01380717832818	20.096488511929653
37	24.39335561464608	28.067058877358082	26.44746068582604	21.0921248221698
38	24.091376791469685	29.254916732372067	25.328713206306443	21.32499326985181
39	24.053124012423908	27.02040112747273	25.596642267884373	23.32983259221899
40	25.808070006309848	25.219434752676364	26.47975475659209	22.492740484421702
41	21.870481091209733	24.568126656600704	27.194586939149882	26.366805313039677
42	24.43575820838667	25.480500659182105	25.781043008041067	24.30269812439016
43	23.269021846114228	24.29594137482297	27.967622932940056	24.467413846122742
44	23.582545666582604	25.590949573367126	27.161601232601367	23.664903527448896
45	22.659424707996692	26.077329136700218	25.97757397576721	25.28567217953588
46	24.74784555453761	25.773275406176417	26.30679260822238	23.172086431063597
47	23.50034741397381	25.23018170868088	28.043170841486653	23.226300035858653
48	24.191397966165177	24.58712003924235	28.415802919979864	22.805679074612602
49	23.941823854198923	23.96709516163528	28.49661790102373	23.59446308314207
50	23.526203951687645	25.380532687239103	28.191819331964957	22.90144402910829
51	22.962946410995517	26.040619237476868	26.2752433759913	24.72119097553631
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1171.0
1	1929.5
2	2688.0
3	9497.5
4	16307.0
5	11425.0
6	6543.0
7	6860.0
8	7177.0
9	7704.0
10	8231.0
11	8444.0
12	8657.0
13	8697.5
14	8738.0
15	8613.0
16	8488.0
17	8434.0
18	8380.0
19	8419.5
20	8459.0
21	8315.0
22	8171.0
23	8354.5
24	8538.0
25	8887.5
26	10959.0
27	12681.0
28	14222.0
29	15763.0
30	17665.5
31	19568.0
32	24488.5
33	29409.0
34	32395.5
35	35382.0
36	39756.0
37	44130.0
38	48927.5
39	53725.0
40	65399.5
41	77074.0
42	91958.5
43	106843.0
44	121152.0
45	135461.0
46	144761.5
47	154062.0
48	148828.5
49	143595.0
50	138440.5
51	133286.0
52	128217.5
53	123149.0
54	117274.5
55	111400.0
56	106919.0
57	102438.0
58	98354.5
59	94271.0
60	91806.5
61	89342.0
62	80014.5
63	70687.0
64	66830.5
65	62974.0
66	53990.5
67	45007.0
68	40042.5
69	35078.0
70	29261.5
71	23445.0
72	20480.0
73	17515.0
74	16083.0
75	12089.0
76	9527.0
77	6910.0
78	4293.0
79	3175.0
80	2057.0
81	1546.0
82	1035.0
83	781.0
84	527.0
85	363.0
86	199.0
87	156.0
88	113.0
89	91.0
90	69.0
91	46.0
92	23.0
93	24.5
94	26.0
95	17.0
96	8.0
97	5.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1879602.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.949758256563218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58001490838198	18.58022602596319
2	7.1032487092448955	3.4024217884532
3	2.462682306015058	1.7694193767532889
4	1.372751979789273	1.315083122486866
5	0.9094435613923633	1.0890476721667506
6	0.7070824978822182	1.0160672935035597
7	0.5887493595113098	0.9870283383801609
8	0.4543444267793113	0.8705151349265035
9	0.4056728716004497	0.8744190485468922
>10	6.737162761018986	41.21260386510039
>50	1.592644560486959	24.283432502752138
>100	0.08193685282789094	3.09914307147718
>500	0.002020360296017088	0.2995701643035978
>1k	0.002020360296017088	0.6929576180382399
>5k	2.2448447733523205E-4	0.5080649771480616
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9450	0.5027660111023503	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09406246641576249	0.0
2	0.0	0.0	0.0	0.30522419107874965	0.0
3	0.0	0.0	0.0	0.4510529356746801	0.0
4	0.0	0.0	0.0	0.7788350938124134	0.0
5	0.0	0.0	0.0	1.2495730479112068	0.0
6	0.0	0.0	0.0	2.158648479837753	0.0
7	0.0	0.0	0.0	2.5388885519381232	0.0
8	0.0	0.0	0.0	3.594377958738073	0.0
9	0.0	0.0	0.0	4.0771397349013245	0.0
10	0.0	0.0	0.0	4.809741636793321	0.0
11	0.0	0.0	0.0	5.455676254866722	0.0
12	0.0	0.0	0.0	5.951791921906872	0.0
13	0.0	0.0	0.0	6.206526700865396	0.0
14	0.0	0.0	0.0	6.334106901354648	0.0
15	0.0	0.0	0.0	6.483872649635401	0.0
16	0.0	0.0	0.0	6.756217539670632	0.0
17	0.0	0.0	0.0	7.041118279295298	0.0
18	0.0	0.0	0.0	7.409760151351191	0.0
19	0.0	0.0	0.0	7.600172802540112	0.0
20	0.0	0.0	0.0	7.795267295948824	0.0
21	0.0	0.0	0.0	8.037339819812917	0.0
22	0.0	0.0	0.0	8.288190797839118	0.0
23	0.0	0.0	0.0	8.572772320948797	0.0
24	0.0	0.0	0.0	8.798777613558615	0.0
25	0.0	0.0	0.0	8.991158766589948	0.0
26	0.0	0.0	0.0	9.176091534271617	0.0
27	0.0	0.0	0.0	9.378368399267504	0.0
28	5.3202752497603214E-5	0.0	0.0	9.580751669768388	0.0
29	5.3202752497603214E-5	0.0	0.0	9.796808047661154	0.0
30	5.3202752497603214E-5	0.0	0.0	10.068833721181399	0.0
31	5.3202752497603214E-5	0.0	0.0	10.296381893613647	0.0
32	5.3202752497603214E-5	0.0	0.0	10.532176492683025	0.0
33	5.3202752497603214E-5	0.0	0.0	10.751212224715658	0.0
34	5.3202752497603214E-5	0.0	0.0	10.977483531087964	0.0
35	5.3202752497603214E-5	0.0	0.0	11.228600522876652	0.0
36	5.3202752497603214E-5	0.0	0.0	11.443220426451983	0.0
37	5.3202752497603214E-5	0.0	0.0	11.670662193379236	0.0
38	5.3202752497603214E-5	0.0	0.0	11.919544669563024	0.0
39	5.3202752497603214E-5	0.0	0.0	12.244719892828375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	35	1.2124838E-7	45.0	1
CGTTTTT	6055	0.0	40.949627	1
CTCACGA	165	0.0	40.90909	24
ACACGAC	2000	0.0	40.725002	26
CACGACC	2000	0.0	40.612503	27
AGGGCGA	2460	0.0	40.15244	6
GACACGA	2045	0.0	39.938873	25
AGACACG	2045	0.0	39.82885	24
ACGACCA	2045	0.0	39.82885	28
CGAGACA	2065	0.0	39.552055	22
CACGTGA	1920	0.0	39.25781	43
CGACCAA	2085	0.0	39.172657	29
TCAAGCG	2075	0.0	39.144577	17
AACACGT	1955	0.0	39.130432	41
CATACGA	305	0.0	39.098362	18
AGGGTAC	855	0.0	38.94737	6
TAAACGG	110	0.0	38.863636	2
CGTAAGG	325	0.0	38.76923	2
GTCAAGC	2095	0.0	38.663483	16
AAACACG	2050	0.0	38.634148	40
>>END_MODULE