FastQCFastQC Report
Thu 26 May 2016
SRR1547008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547008_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1367150
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67650.49482500091431075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG453.8562575E-1045.01
CACTACG207.0337567E-445.039
CGAATTA253.891196E-545.033
CGCGTAA207.0337567E-445.031
TACGTTC253.891196E-545.039
CGTTTTT43500.041.0689661
TAGCATA7450.040.77181230
GTTTACG501.0822987E-940.51
GTATGCG451.9286745E-840.01
CATATGC7600.039.96710633
ATTAGCG403.458863E-739.3751
GCGATAC750.039.09
ACAACGA2550.038.8235313
AGGGCGA16150.038.591336
AACACGT3800.038.48684341
GGTACCT9000.038.2500048
GTAGCAT8000.037.9687529
AGGGTAC5750.037.956526
GGGCGAT30400.037.8947377
CGTAAGG1900.037.8947372