##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547008_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1367150
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.609767033610066	31.0	31.0	33.0	30.0	34.0
2	31.98777237318509	31.0	31.0	34.0	30.0	34.0
3	32.09435833668581	33.0	31.0	34.0	30.0	34.0
4	35.77262846066635	37.0	35.0	37.0	35.0	37.0
5	35.726653988223674	37.0	35.0	37.0	33.0	37.0
6	35.77426983140109	37.0	35.0	37.0	33.0	37.0
7	36.10038035328969	37.0	35.0	37.0	35.0	37.0
8	36.022195808799324	37.0	35.0	37.0	35.0	37.0
9	37.78131075595216	39.0	38.0	39.0	35.0	39.0
10	37.34346706652525	39.0	37.0	39.0	34.0	39.0
11	37.06614343707713	39.0	37.0	39.0	33.0	39.0
12	36.53166953150715	39.0	35.0	39.0	33.0	39.0
13	36.34299893939948	39.0	35.0	39.0	32.0	39.0
14	37.40690048641334	40.0	35.0	41.0	32.0	41.0
15	37.552792305160374	40.0	35.0	41.0	33.0	41.0
16	37.641004278974506	40.0	35.0	41.0	33.0	41.0
17	37.59660242109498	40.0	35.0	41.0	33.0	41.0
18	37.508051055114656	39.0	35.0	41.0	33.0	41.0
19	37.44603006253886	39.0	35.0	41.0	33.0	41.0
20	37.304644698826024	39.0	35.0	41.0	32.0	41.0
21	37.21739750576016	39.0	35.0	41.0	32.0	41.0
22	37.130401931024394	39.0	35.0	41.0	32.0	41.0
23	37.064549610503605	39.0	35.0	41.0	32.0	41.0
24	36.93574004315547	39.0	35.0	41.0	32.0	41.0
25	36.818469078008995	38.0	35.0	41.0	31.0	41.0
26	36.656524887539774	38.0	35.0	41.0	31.0	41.0
27	36.548502358921844	38.0	35.0	41.0	31.0	41.0
28	36.59709761182021	38.0	35.0	41.0	31.0	41.0
29	36.589618549537356	38.0	35.0	41.0	31.0	41.0
30	36.50579599897597	38.0	35.0	41.0	31.0	41.0
31	36.355797096148926	38.0	35.0	41.0	31.0	41.0
32	36.26204586182935	38.0	35.0	41.0	30.0	41.0
33	36.11720732911531	38.0	35.0	41.0	30.0	41.0
34	35.9500128003511	38.0	35.0	41.0	30.0	41.0
35	35.83101634787697	38.0	35.0	41.0	29.0	41.0
36	35.70405441977837	38.0	35.0	41.0	29.0	41.0
37	35.60911823867169	38.0	35.0	41.0	29.0	41.0
38	35.49511246022748	38.0	35.0	40.0	28.0	41.0
39	35.38282997476502	38.0	35.0	40.0	27.0	41.0
40	35.208302673444756	37.0	34.0	40.0	27.0	41.0
41	35.11260724865596	37.0	34.0	40.0	26.0	41.0
42	35.02091357934389	37.0	34.0	40.0	26.0	41.0
43	34.88471857513806	37.0	34.0	40.0	26.0	41.0
44	34.740186519401675	36.0	34.0	40.0	26.0	41.0
45	34.620721208353146	36.0	34.0	40.0	25.0	41.0
46	34.55290202245547	36.0	34.0	40.0	25.0	41.0
47	34.48115568884175	36.0	34.0	40.0	25.0	41.0
48	34.37220787770179	35.0	34.0	40.0	24.0	41.0
49	34.2984756610467	36.0	34.0	40.0	24.0	41.0
50	34.16634897414329	35.0	34.0	40.0	24.0	41.0
51	33.78349047288154	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	1.0
12	3.0
13	6.0
14	21.0
15	37.0
16	113.0
17	291.0
18	622.0
19	1279.0
20	2115.0
21	3337.0
22	4944.0
23	7351.0
24	10593.0
25	14530.0
26	18732.0
27	22055.0
28	24334.0
29	27073.0
30	31417.0
31	37166.0
32	45168.0
33	59517.0
34	103880.0
35	168884.0
36	103823.0
37	134399.0
38	193645.0
39	351534.0
40	277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.641809603920564	24.66649599531873	26.562703434151334	13.128990966609368
2	32.55465749917712	26.376184032476317	27.388582086822954	13.680576381523608
3	30.02560070219069	26.027063599458728	29.89196503675529	14.05537066159529
4	25.773762937497715	29.09000475441612	29.390118128954395	15.746114179131771
5	23.911202135830013	32.914457082251396	28.15821233953846	15.016128442380133
6	22.694437333138282	41.58022162893611	25.222031232856672	10.50330980506894
7	87.57905131112167	3.9474819880773873	6.661522144607394	1.8119445561935412
8	86.96229382291628	3.6619244413561054	6.415462824123177	2.9603189116044324
9	81.39604286288996	5.494568993892404	9.526313864608857	3.583074278608785
10	47.31638810664521	23.579782759755695	14.971363785978129	14.132465347620963
11	37.811213107559524	24.63723804995794	21.389020956003364	16.16252788647917
12	32.489704860476174	21.831108510404857	26.044033207767985	19.635153421350985
13	23.92129612697948	29.034560947957427	28.71923344183155	18.32490948323154
14	20.793841202501554	30.58062392568482	29.575979226858795	19.049555644954832
15	19.665581684526202	22.986870497019346	38.48195150495556	18.865596313498884
16	22.485243023808653	21.028489924295066	35.835643491935784	20.6506235599605
17	23.05650440697802	21.408623779395093	27.428153457923415	28.10671835570347
18	24.32161796437845	22.61522144607395	31.274256665325677	21.788903924221923
19	28.257762498628537	24.425410525545843	25.715247046776142	21.601579929049482
20	29.357714954467323	24.226237062502285	24.728303404893392	21.687744578137
21	26.281680868960976	26.487291079983912	26.842994550707676	20.38803350034744
22	25.497641078155286	23.190652086457227	26.062831437662293	25.248875397725197
23	23.975569615623744	26.388691804118057	25.061331967962552	24.57440661229565
24	23.93380389862122	23.66090041326848	31.68993892403906	20.715356764071245
25	23.776323007716783	23.78919650367553	29.125041143985662	23.309439344622024
26	21.56054566068098	27.504809274768675	26.83743554108913	24.097209523461217
27	22.272537761035732	26.49372782796328	27.560691950407783	23.673042460593205
28	20.877592071096807	26.836996671908715	30.67834546318985	21.60706579380463
29	22.233185824525474	24.796766997037633	29.78275975569616	23.187287422740738
30	24.112057930731815	26.009143107925247	28.174157919760084	21.704641041582857
31	27.151885308854183	26.267051896280584	24.30559923929342	22.275463555571807
32	28.391910178107743	25.288081044508647	24.2378670957832	22.082141681600408
33	26.748564532055735	26.175181947847715	24.26544270928574	22.81081081081081
34	22.680978678272318	26.740372307354715	25.999487985956186	24.57916102841678
35	22.565921808141024	27.57817357276085	27.119555279230518	22.736349339867605
36	27.999414841092786	26.6878542954321	23.605456606809785	21.707274256665325
37	23.49288666203416	29.384193395018837	25.030757415060528	22.09216252788648
38	23.40818491021468	29.641882748783964	23.98961342939692	22.960318911604432
39	23.293713198990602	28.205683355886336	24.339611600775335	24.16099184434773
40	25.428738616830632	25.240975752477784	25.753794389788975	23.57649124090261
41	21.354423435614233	24.895658852357094	26.543978349120433	27.20593936290824
42	23.951212376110888	25.8549537358739	25.165782832900558	25.028051055114652
43	23.36839410452401	24.30991478623414	27.447683136451744	24.87400797279011
44	22.578502724646164	25.935047361299052	26.92893976520499	24.557510148849797
45	22.057052993453535	27.24719306586695	25.657023735508172	25.038730205171344
46	24.338514427824308	26.051567128698387	26.372161064989214	23.237757378488098
47	23.35983615550598	25.857879530409978	26.994404417949752	23.787879896134296
48	23.40438137731778	24.958270855429177	28.35833668580624	23.279011081446804
49	23.79109827012398	23.802435723951284	28.096551219690596	24.30991478623414
50	22.394909117507222	26.663423911055844	27.258969388874664	23.682697582562266
51	21.827378122371357	27.493471820941373	26.002267490765462	24.676882565921808
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	930.0
1	1503.5
2	2077.0
3	6818.0
4	11559.0
5	8256.0
6	4953.0
7	5050.5
8	5148.0
9	5665.0
10	6182.0
11	6393.0
12	6604.0
13	6702.5
14	6801.0
15	6757.5
16	6714.0
17	6643.0
18	6572.0
19	6341.5
20	6111.0
21	6139.5
22	6168.0
23	6214.5
24	6261.0
25	6922.5
26	8508.0
27	9432.0
28	10566.0
29	11700.0
30	12645.0
31	13590.0
32	16704.5
33	19819.0
34	22126.0
35	24433.0
36	28326.5
37	32220.0
38	35632.5
39	39045.0
40	51326.0
41	63607.0
42	73999.5
43	84392.0
44	92301.5
45	100211.0
46	104021.0
47	107831.0
48	107378.0
49	106925.0
50	100044.5
51	93164.0
52	87035.0
53	80906.0
54	77463.5
55	74021.0
56	72778.0
57	71535.0
58	68922.0
59	66309.0
60	64428.0
61	62547.0
62	58933.0
63	55319.0
64	51235.5
65	47152.0
66	41459.5
67	35767.0
68	31531.0
69	27295.0
70	23246.5
71	19198.0
72	16529.0
73	13860.0
74	11654.0
75	7985.0
76	6522.0
77	5307.0
78	4092.0
79	2886.0
80	1680.0
81	1228.5
82	777.0
83	556.5
84	336.0
85	271.0
86	206.0
87	152.0
88	98.0
89	64.5
90	31.0
91	23.0
92	15.0
93	8.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1367150.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.617916667431523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63038916311709	19.357163679453272
2	6.432342509837592	3.1670174376711833
3	2.2233098387747448	1.6419976901051185
4	1.2827003810351014	1.2630964435841914
5	0.9191220661796031	1.1313435216203476
6	0.655332197854	0.9679748061752711
7	0.54562098959581	0.940243643770982
8	0.4431839809185047	0.8728213048473846
9	0.4034310184568016	0.8938468074083914
>10	7.000292199611606	44.726068705376655
>50	1.3993764621863587	21.37590354474525
>100	0.061011222379347356	2.3972606260194276
>500	0.002392596955958697	0.41650194597802925
>1k	0.0011962984779793485	0.35068035508102413
>5k	2.9907461949483713E-4	0.49807948816357933
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6765	0.49482500091431075	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0706579380463007	0.0
2	0.0	0.0	0.0	0.2264564970924917	0.0
3	0.0	0.0	0.0	0.3464872179351205	0.0
4	0.0	0.0	0.0	0.638847236952785	0.0
5	0.0	0.0	0.0	1.0366090041326848	0.0
6	0.0	0.0	0.0	1.8003145229126285	0.0
7	0.0	0.0	0.0	2.1182020992575796	0.0
8	0.0	0.0	0.0	3.070401931024394	0.0
9	0.0	0.0	0.0	3.4813297736166477	0.0
10	0.0	0.0	0.0	4.103280547123578	0.0
11	0.0	0.0	0.0	4.702629557839301	0.0
12	0.0	0.0	0.0	5.156274000658303	0.0
13	0.0	0.0	0.0	5.379658413487912	0.0
14	0.0	0.0	0.0	5.489009984273855	0.0
15	0.0	0.0	0.0	5.6097721537505025	0.0
16	0.0	0.0	0.0	5.847346670080094	0.0
17	0.0	0.0	0.0	6.091577368979263	0.0
18	0.0	0.0	0.0	6.418681198112862	0.0
19	0.0	0.0	0.0	6.583403430494093	0.0
20	0.0	0.0	0.0	6.768533079764474	0.0
21	0.0	0.0	0.0	6.998354240573455	0.0
22	0.0	0.0	0.0	7.220714625315437	0.0
23	0.0	0.0	0.0	7.4820612222506675	0.0
24	7.31448634019676E-5	0.0	0.0	7.688256592180814	0.0
25	7.31448634019676E-5	0.0	0.0	7.86914383937388	0.0
26	7.31448634019676E-5	0.0	0.0	8.03905935705665	0.0
27	7.31448634019676E-5	0.0	0.0	8.197564276048714	0.0
28	7.31448634019676E-5	0.0	0.0	8.370551877994368	0.0
29	7.31448634019676E-5	0.0	0.0	8.557290714259592	0.0
30	7.31448634019676E-5	0.0	0.0	8.78996452474125	0.0
31	7.31448634019676E-5	0.0	0.0	8.9925026515013	0.0
32	7.31448634019676E-5	0.0	0.0	9.178290604542296	0.0
33	7.31448634019676E-5	0.0	0.0	9.392312474856453	0.0
34	7.31448634019676E-5	0.0	0.0	9.59068134440259	0.0
35	7.31448634019676E-5	0.0	0.0	9.828841019639396	0.0
36	7.31448634019676E-5	0.0	0.0	10.029184800497385	0.0
37	1.462897268039352E-4	0.0	0.0	10.236623633105365	0.0
38	1.462897268039352E-4	0.0	0.0	10.474929598068975	0.0
39	1.462897268039352E-4	0.0	0.0	10.768240500310865	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	45	3.8562575E-10	45.0	1
CACTACG	20	7.0337567E-4	45.0	39
CGAATTA	25	3.891196E-5	45.0	33
CGCGTAA	20	7.0337567E-4	45.0	31
TACGTTC	25	3.891196E-5	45.0	39
CGTTTTT	4350	0.0	41.068966	1
TAGCATA	745	0.0	40.771812	30
GTTTACG	50	1.0822987E-9	40.5	1
GTATGCG	45	1.9286745E-8	40.0	1
CATATGC	760	0.0	39.967106	33
ATTAGCG	40	3.458863E-7	39.375	1
GCGATAC	75	0.0	39.0	9
ACAACGA	255	0.0	38.82353	13
AGGGCGA	1615	0.0	38.59133	6
AACACGT	380	0.0	38.486843	41
GGTACCT	900	0.0	38.250004	8
GTAGCAT	800	0.0	37.96875	29
AGGGTAC	575	0.0	37.95652	6
GGGCGAT	3040	0.0	37.894737	7
CGTAAGG	190	0.0	37.894737	2
>>END_MODULE