FastQCFastQC Report
Thu 26 May 2016
SRR1547007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547007_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3247678
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170730.5256986684024709No Hit
GAGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT36420.1121416593640133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106400.040.432331
CGTTAGG4750.039.7894742
TAGGGCG17350.038.515855
AGGGCGA42950.038.451696
ACAACGA5450.038.39449313
CGTAAGG4500.037.52
GTTGATC19400.037.34536416
GGGCGAT78750.037.3142857
TCGTTAG1700.037.0588231
AGGGTAC13600.037.0588236
ACGGGTA2500.036.95
ACGGGAT6650.036.87975
TATACGG3550.036.1267622
GCCAGTA19550.036.02301425
CGGTCTA2250.036.031
TAGCATA19300.035.90673430
GCGTAAG2450.035.8163261
GCCGATG4950.035.4545449
TCACGAC2350.035.42553325
CATATGC19800.035.34090833
Produced by FastQC (version 0.11.5)