##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547007_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3247678
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.612868948214693	31.0	31.0	33.0	30.0	34.0
2	31.99657047281165	31.0	31.0	34.0	30.0	34.0
3	32.08444310057832	33.0	31.0	34.0	30.0	34.0
4	35.76306949149515	37.0	35.0	37.0	35.0	37.0
5	35.7086444530523	37.0	35.0	37.0	33.0	37.0
6	35.76230001865949	37.0	35.0	37.0	33.0	37.0
7	36.10549721985985	37.0	35.0	37.0	35.0	37.0
8	36.03250322230221	37.0	35.0	37.0	35.0	37.0
9	37.79311957650974	39.0	38.0	39.0	35.0	39.0
10	37.33586088275993	39.0	37.0	39.0	34.0	39.0
11	37.07937917490589	39.0	37.0	39.0	33.0	39.0
12	36.62818358223937	39.0	35.0	39.0	33.0	39.0
13	36.49039067296696	39.0	35.0	39.0	32.0	39.0
14	37.52382871700951	40.0	35.0	41.0	32.0	41.0
15	37.634456987423015	40.0	35.0	41.0	33.0	41.0
16	37.71980381059945	40.0	35.0	41.0	33.0	41.0
17	37.672848416622585	40.0	35.0	41.0	33.0	41.0
18	37.58111118158882	39.0	36.0	41.0	33.0	41.0
19	37.5101915891908	39.0	35.0	41.0	33.0	41.0
20	37.38159694403201	39.0	35.0	41.0	32.0	41.0
21	37.31195518767563	39.0	35.0	41.0	32.0	41.0
22	37.236548081429255	39.0	35.0	41.0	32.0	41.0
23	37.1469653087529	39.0	35.0	41.0	32.0	41.0
24	37.042114088896746	39.0	35.0	41.0	32.0	41.0
25	36.92180413205989	39.0	35.0	41.0	32.0	41.0
26	36.762646419996074	38.0	35.0	41.0	31.0	41.0
27	36.64852827158357	38.0	35.0	41.0	31.0	41.0
28	36.695598208935735	38.0	35.0	41.0	31.0	41.0
29	36.68025894192712	38.0	35.0	41.0	31.0	41.0
30	36.580891332207194	38.0	35.0	41.0	31.0	41.0
31	36.46326359940856	38.0	35.0	41.0	31.0	41.0
32	36.3594759086338	38.0	35.0	41.0	30.0	41.0
33	36.22558886687658	38.0	35.0	41.0	30.0	41.0
34	36.05225887541807	38.0	35.0	41.0	30.0	41.0
35	35.92557082321585	38.0	35.0	41.0	30.0	41.0
36	35.83782474740415	38.0	35.0	41.0	29.0	41.0
37	35.7511191072514	38.0	35.0	41.0	29.0	41.0
38	35.64262528489586	38.0	35.0	41.0	29.0	41.0
39	35.525840000147795	38.0	35.0	40.0	28.0	41.0
40	35.374956507387736	38.0	35.0	40.0	27.0	41.0
41	35.3016586003908	38.0	35.0	40.0	27.0	41.0
42	35.20814409556613	37.0	34.0	40.0	27.0	41.0
43	35.083320452335485	37.0	34.0	40.0	26.0	41.0
44	34.865716983026026	37.0	34.0	40.0	26.0	41.0
45	34.784017381033465	37.0	34.0	40.0	25.0	41.0
46	34.74828015585289	37.0	34.0	40.0	25.0	41.0
47	34.685965480567965	36.0	34.0	40.0	25.0	41.0
48	34.58915600622968	36.0	34.0	40.0	25.0	41.0
49	34.51418983039575	36.0	34.0	40.0	25.0	41.0
50	34.40642237315399	36.0	34.0	40.0	24.0	41.0
51	34.029215334771486	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	8.0
12	6.0
13	13.0
14	32.0
15	99.0
16	258.0
17	657.0
18	1371.0
19	2645.0
20	4516.0
21	7271.0
22	11078.0
23	16240.0
24	24310.0
25	33932.0
26	43294.0
27	50587.0
28	55036.0
29	62756.0
30	73313.0
31	86989.0
32	107393.0
33	140012.0
34	238932.0
35	374792.0
36	249169.0
37	322558.0
38	467419.0
39	872319.0
40	671.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.200712016400644	24.465387270536056	27.93780664216095	13.396094070902349
2	32.61579503879387	26.028873552119393	27.775506069259333	13.579825339827408
3	30.350761374742202	25.487502147688286	30.118595501154978	14.043140976414534
4	26.038511207083953	29.133891968354003	29.05928481826092	15.768312006301116
5	23.99748374069104	32.38224355986031	28.257665938556713	15.362606760891936
6	22.336142930425986	41.63574713995661	25.504529697833345	10.523580231784061
7	86.69917399446621	3.9127955419225673	7.433834265589138	1.9541961980220945
8	86.07660611673941	3.5569720889817282	6.956139124629966	3.4102826696489
9	80.31781475872916	5.396871241545497	10.168957636810052	4.116356362915289
10	45.28084372896574	24.544859434956297	15.761537935718998	14.412758900358963
11	35.376444339617414	25.35220548342539	22.970596222901406	16.300753954055793
12	31.17824488757814	21.686294022991195	27.474614170493506	19.66084691893716
13	24.221028069901017	27.570313313080913	29.864968140314403	18.343690476703664
14	21.053472665701463	29.50076947283567	30.61448825899612	18.831269602466747
15	20.053465891630882	23.859723778034645	37.581804600086585	18.505005730247888
16	23.06244646174898	21.60137181087534	35.56590277730736	19.770278950068327
17	22.71946295168425	22.778489739438452	28.136533240056433	26.36551406882086
18	24.26952425702302	23.24716305003144	31.048552227160453	21.434760465785093
19	27.320473273520342	25.657438945609755	26.330843143932377	20.69124463693753
20	28.54291589252383	25.02378622511222	25.13913017238778	21.294167709976175
21	25.89567069149097	26.722138093739588	27.83952103626037	19.542670178509074
22	25.429429888061563	23.270995461988534	26.87477022044673	24.424804429503173
23	23.576875540001193	26.983247723450415	25.689184703655965	23.750692032892424
24	23.243776014740376	24.759166395190658	31.286876346731418	20.710181243337548
25	23.95240538009002	24.33643359963642	28.835832862740702	22.87532815753286
26	21.19535865316697	27.319364789243267	27.407981948949374	24.07729460864039
27	21.998640259286788	26.399569169110976	28.098198158807612	23.503592412794617
28	20.930738823245406	27.142253634750734	30.097719047270083	21.829288494733774
29	22.18135541762453	25.021784795167502	29.41178281837054	23.385076968837428
30	24.150516153387127	26.011630463364906	28.691083290892756	21.146770092355215
31	26.513773840879544	25.504991566282126	25.94598356117817	22.03525103166016
32	28.453929238058702	25.36267450159776	25.532580508289303	20.650815752054235
33	26.576064499005135	24.596527118759926	26.756624271248562	22.07078411098637
34	23.758359049142186	26.02597917650703	28.249937339847115	21.965724434503667
35	22.312649221997994	25.730598907896656	29.376372903963997	22.58037896614135
36	27.56606412335213	25.65180415053463	26.232680702951463	20.54945102316178
37	23.938426161706918	26.389685184307066	28.101554402868757	21.57033425111726
38	24.845751333722124	26.685434947676463	26.738980896505133	21.729832822096277
39	23.73794446370607	25.61319194821654	26.94001683664452	23.70884675143287
40	25.224206340653232	23.546361431151734	27.655389481346365	23.574042746848672
41	21.210754268126337	23.581956092937787	28.526812079276333	26.68047755965955
42	23.735419582852735	24.927409675466595	27.313883950317734	24.023286791362935
43	22.822521198222237	23.66715542612291	29.05983906039946	24.45048431525539
44	23.240512144368992	25.097315682158143	27.44970406548925	24.21246810798361
45	22.043349125128785	25.782235800470367	27.100716265590368	25.07369880881048
46	24.078895752596164	25.070127026139904	27.68100778463875	23.16996943662518
47	22.924840455242176	24.722617205277125	29.107811796612843	23.24473054286786
48	23.164180685400463	23.872871633209943	29.826417520456154	23.13653016093344
49	23.85476639001773	22.569263332140686	29.61851513604489	23.957455141796693
50	22.794932256215056	24.682834936222125	28.483642774930274	24.038590032632545
51	21.880986969767324	25.730814446506088	27.228838573282204	25.159360010444388
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2420.0
1	3885.5
2	5351.0
3	17270.5
4	29190.0
5	20557.5
6	11925.0
7	12527.5
8	13130.0
9	14000.0
10	14870.0
11	15335.0
12	15800.0
13	16200.5
14	16601.0
15	16463.5
16	16326.0
17	16224.0
18	16122.0
19	16150.5
20	16179.0
21	16215.0
22	16251.0
23	16918.0
24	17585.0
25	17906.5
26	21052.0
27	23876.0
28	26999.5
29	30123.0
30	33714.5
31	37306.0
32	43554.5
33	49803.0
34	55714.0
35	61625.0
36	69321.5
37	77018.0
38	85584.5
39	94151.0
40	117791.0
41	141431.0
42	165249.5
43	189068.0
44	221823.5
45	254579.0
46	266000.0
47	277421.0
48	269705.0
49	261989.0
50	243106.5
51	224224.0
52	213340.5
53	202457.0
54	191887.0
55	181317.0
56	173301.5
57	165286.0
58	158969.5
59	152653.0
60	148395.0
61	144137.0
62	133839.5
63	123542.0
64	111836.5
65	100131.0
66	88658.5
67	77186.0
68	65434.5
69	53683.0
70	46046.5
71	38410.0
72	34191.0
73	29972.0
74	24987.0
75	16581.0
76	13160.0
77	10045.5
78	6931.0
79	5097.0
80	3263.0
81	2415.5
82	1568.0
83	1201.0
84	834.0
85	555.0
86	276.0
87	220.0
88	164.0
89	111.5
90	59.0
91	44.5
92	30.0
93	23.0
94	16.0
95	10.0
96	4.0
97	4.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3247678.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.10923360034953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37019478121805	16.543347489416256
2	8.181602800780388	3.4541472949389433
3	2.8150610015487327	1.7827134084277834
4	1.396238143183803	1.1789406850474071
5	0.8760883177349087	0.9246776476801715
6	0.6400286936802967	0.8106309123494362
7	0.47167946480369666	0.6969754404920353
8	0.3799457541982036	0.6416290944664691
9	0.3138177139641104	0.5962006288796469
>10	3.860039102808319	20.006919787326442
>50	1.8881349473539648	29.22391288431253
>100	0.8007945698353616	21.89867406125439
>500	0.0032614789670570086	0.47041497057192716
>1k	0.002964980879142735	1.2365291865492654
>5k	0.0	0.0
>10k+	1.4824904395713674E-4	0.534286508287288
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17073	0.5256986684024709	No Hit
GAGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	3642	0.1121416593640133	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.158245983745926E-5	0.0	0.0	0.09668446194481103	0.0
2	6.158245983745926E-5	0.0	0.0	0.33488541659610344	0.0
3	6.158245983745926E-5	0.0	0.0	0.5189553890502692	0.0
4	6.158245983745926E-5	0.0	0.0	0.9479080130480916	0.0
5	6.158245983745926E-5	0.0	0.0	1.5555113530343834	0.0
6	6.158245983745926E-5	0.0	0.0	2.6055538757229013	0.0
7	6.158245983745926E-5	0.0	0.0	3.059816890713919	0.0
8	6.158245983745926E-5	0.0	0.0	4.216581816300754	0.0
9	6.158245983745926E-5	0.0	0.0	4.764634917624223	0.0
10	6.158245983745926E-5	0.0	0.0	5.610931871940506	0.0
11	6.158245983745926E-5	0.0	0.0	6.420310141584234	0.0
12	6.158245983745926E-5	0.0	0.0	7.0465113844414375	0.0
13	6.158245983745926E-5	0.0	0.0	7.362183073568254	0.0
14	6.158245983745926E-5	0.0	0.0	7.507517678784658	0.0
15	9.237368975618888E-5	0.0	0.0	7.66236677404595	0.0
16	1.5395614959364814E-4	0.0	0.0	7.959378977842015	0.0
17	1.5395614959364814E-4	0.0	0.0	8.264458483876789	0.0
18	1.5395614959364814E-4	0.0	0.0	8.667084606294097	0.0
19	1.5395614959364814E-4	0.0	0.0	8.876803673270564	0.0
20	1.5395614959364814E-4	0.0	0.0	9.103519499162172	0.0
21	1.5395614959364814E-4	0.0	0.0	9.383750482652529	0.0
22	1.5395614959364814E-4	0.0	0.0	9.670416833195901	0.0
23	1.5395614959364814E-4	0.0	0.0	9.98140825537507	0.0
24	1.5395614959364814E-4	0.0	0.0	10.23571302327386	0.0
25	1.5395614959364814E-4	0.0	0.0	10.443584616455203	0.0
26	1.5395614959364814E-4	0.0	0.0	10.642742291569546	0.0
27	1.5395614959364814E-4	0.0	0.0	10.858280901000654	0.0
28	1.5395614959364814E-4	0.0	0.0	11.064027899317605	0.0
29	1.5395614959364814E-4	0.0	0.0	11.298133620389706	0.0
30	1.5395614959364814E-4	0.0	0.0	11.600626663111306	0.0
31	1.5395614959364814E-4	0.0	0.0	11.838889200222436	0.0
32	1.5395614959364814E-4	0.0	0.0	12.081062223533245	0.0
33	1.5395614959364814E-4	0.0	0.0	12.325606171547795	0.0
34	1.5395614959364814E-4	0.0	0.0	12.576062035706743	0.0
35	1.5395614959364814E-4	0.0	0.0	12.867285488278087	0.0
36	1.5395614959364814E-4	0.0	0.0	13.11198339244223	0.0
37	1.5395614959364814E-4	0.0	0.0	13.361084442484755	0.0
38	1.5395614959364814E-4	0.0	0.0	13.652800554734798	0.0
39	1.5395614959364814E-4	0.0	0.0	14.059768240570648	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	10640	0.0	40.43233	1
CGTTAGG	475	0.0	39.789474	2
TAGGGCG	1735	0.0	38.51585	5
AGGGCGA	4295	0.0	38.45169	6
ACAACGA	545	0.0	38.394493	13
CGTAAGG	450	0.0	37.5	2
GTTGATC	1940	0.0	37.345364	16
GGGCGAT	7875	0.0	37.314285	7
TCGTTAG	170	0.0	37.058823	1
AGGGTAC	1360	0.0	37.058823	6
ACGGGTA	250	0.0	36.9	5
ACGGGAT	665	0.0	36.8797	5
TATACGG	355	0.0	36.126762	2
GCCAGTA	1955	0.0	36.023014	25
CGGTCTA	225	0.0	36.0	31
TAGCATA	1930	0.0	35.906734	30
GCGTAAG	245	0.0	35.816326	1
GCCGATG	495	0.0	35.454544	9
TCACGAC	235	0.0	35.425533	25
CATATGC	1980	0.0	35.340908	33
>>END_MODULE