FastQCFastQC Report
Thu 26 May 2016
SRR1547006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547006_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1727939
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94030.5441743024493341No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG20170.11672865766673476No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG253.8916234E-545.01
CGTTTTT58350.041.4138831
ATTTACG603.6379788E-1241.2499961
GCATATC3300.038.8636428
TTCGACG3250.038.7692325
TGCGATA356.2501167E-638.57142612
TCGTACA356.2501167E-638.57142634
ATATGCT13050.038.44827334
AGGGTAC9600.038.4374966
AGGGCGA21850.038.409616
CATATGC13350.038.25842733
ATGACGG1950.038.0769232
GGGCGAT42900.037.867137
TTGATCC13850.037.85198617
TAGCATA13550.037.5276730
GCTACGA900.037.510
GTATACG301.1401597E-437.4999961
TAATGCG301.1401597E-437.4999961
CATACGA2600.037.2115418
AGCATAT13700.037.1167931
Produced by FastQC (version 0.11.5)