##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547006_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1727939
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.609147660883863	31.0	31.0	33.0	30.0	34.0
2	31.99580598620669	31.0	31.0	34.0	30.0	34.0
3	32.0956648353906	33.0	31.0	34.0	30.0	34.0
4	35.77817561846801	37.0	35.0	37.0	35.0	37.0
5	35.721124993417014	37.0	35.0	37.0	33.0	37.0
6	35.77344975719629	37.0	35.0	37.0	33.0	37.0
7	36.09699069237976	37.0	35.0	37.0	35.0	37.0
8	36.0273470301903	37.0	35.0	37.0	35.0	37.0
9	37.78947347099637	39.0	38.0	39.0	35.0	39.0
10	37.35516010692507	39.0	37.0	39.0	34.0	39.0
11	37.06605673001188	39.0	37.0	39.0	33.0	39.0
12	36.50537316421471	39.0	35.0	39.0	33.0	39.0
13	36.31242943182601	39.0	35.0	39.0	32.0	39.0
14	37.31452904298126	40.0	35.0	41.0	32.0	41.0
15	37.472701293274824	40.0	35.0	41.0	32.0	41.0
16	37.58171555824598	40.0	35.0	41.0	33.0	41.0
17	37.53924588773099	39.0	35.0	41.0	33.0	41.0
18	37.44845159464541	39.0	35.0	41.0	33.0	41.0
19	37.37530954507075	39.0	35.0	41.0	32.0	41.0
20	37.22179486660119	39.0	35.0	41.0	32.0	41.0
21	37.15508822938773	39.0	35.0	41.0	32.0	41.0
22	37.072559853096664	39.0	35.0	41.0	32.0	41.0
23	36.976824992085945	39.0	35.0	41.0	32.0	41.0
24	36.86857174934995	38.0	35.0	41.0	32.0	41.0
25	36.71961162980869	38.0	35.0	41.0	31.0	41.0
26	36.55197608248902	38.0	35.0	41.0	31.0	41.0
27	36.440296792884475	38.0	35.0	41.0	31.0	41.0
28	36.4602205286182	38.0	35.0	41.0	31.0	41.0
29	36.450067971149444	38.0	35.0	41.0	31.0	41.0
30	36.35704096035798	38.0	35.0	40.0	31.0	41.0
31	36.21448152972993	38.0	35.0	40.0	30.0	41.0
32	36.088275685657884	38.0	35.0	41.0	30.0	41.0
33	35.95701700117886	38.0	35.0	41.0	30.0	41.0
34	35.743052850824014	38.0	35.0	41.0	29.0	41.0
35	35.599665265961356	38.0	35.0	41.0	29.0	41.0
36	35.45395989094522	38.0	35.0	40.0	28.0	41.0
37	35.34813902574107	38.0	34.0	40.0	27.0	41.0
38	35.23520448349161	37.0	34.0	40.0	27.0	41.0
39	35.10912422255647	37.0	34.0	40.0	27.0	41.0
40	34.933256324442006	37.0	34.0	40.0	26.0	41.0
41	34.84638635970367	37.0	34.0	40.0	26.0	41.0
42	34.72384499684306	36.0	34.0	40.0	25.0	41.0
43	34.584662421532244	36.0	34.0	40.0	25.0	41.0
44	34.410239018854256	36.0	34.0	40.0	24.0	41.0
45	34.28519351666928	35.0	34.0	40.0	24.0	41.0
46	34.22972107232952	35.0	34.0	40.0	24.0	41.0
47	34.13485140389794	35.0	33.0	40.0	23.0	41.0
48	34.00316388483621	35.0	33.0	40.0	23.0	41.0
49	33.91141006713779	35.0	33.0	39.0	24.0	41.0
50	33.78915864506791	35.0	33.0	39.0	24.0	41.0
51	33.437047256876546	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	1.0
13	10.0
14	28.0
15	51.0
16	141.0
17	335.0
18	819.0
19	1642.0
20	2743.0
21	4369.0
22	6541.0
23	9475.0
24	13693.0
25	19088.0
26	24924.0
27	29000.0
28	32342.0
29	36138.0
30	41465.0
31	49482.0
32	60529.0
33	79049.0
34	138358.0
35	213076.0
36	135312.0
37	178388.0
38	250797.0
39	399847.0
40	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.48541875610192	24.123189533889796	27.488412496042976	13.902979213965308
2	32.40982465237488	26.25092668201829	27.831885269098038	13.50736339650879
3	30.22369423920636	25.547661115351872	30.298291779976026	13.930352865465737
4	26.380908122335338	28.50847165322387	29.78722049794582	15.323399726494975
5	23.779369526354806	32.66428965374356	28.80211627841029	14.754224541491338
6	22.883793930225547	41.24080769054926	25.010200012847676	10.865198366377516
7	87.33520106901922	3.891456816473267	6.96205132241358	1.811290792093934
8	87.02020152331767	3.5259925263565437	6.658452642136094	2.7953533081896986
9	81.58875978839531	5.158920540597787	9.362425409693282	3.8898942613136227
10	49.06093328526065	22.86099219937741	14.769907965501098	13.308166549860845
11	39.23234558627359	23.531907086997865	21.097156786205996	16.138590540522554
12	33.235374628386765	19.86754162039285	26.955696931431028	19.941386819789354
13	25.3997970993189	26.741800491799765	28.87694530883324	18.98145710004809
14	21.413429525000595	28.7734115614035	30.32624415560966	19.48691475798625
15	20.567566331913337	21.239522922973553	38.346839790062035	19.846070955051076
16	24.221225402054124	18.836949683987687	35.475673620422945	21.466151293535248
17	24.1565240439622	19.905332306290905	27.782983079842516	28.155160569904375
18	25.880658981595992	21.077885272570384	30.39748509640676	22.643970649426862
19	28.597710914563535	23.37970263996588	25.41964733708771	22.602939108382877
20	29.849433342264973	23.40481926734682	24.755966501132274	21.98978088925593
21	27.27584712191808	24.555207099324686	27.172776353794898	20.99616942496234
22	26.854709570187374	21.26573912620758	26.19901512727012	25.68053617633493
23	24.93791736861081	24.55740625103085	25.25332202120561	25.25135435915272
24	25.19053045275325	22.645185970106585	30.56068530196957	21.603598275170594
25	25.236828383409364	22.21936075289695	28.495450360226837	24.04836050346685
26	22.381345637780036	25.910289657215905	26.755458381343324	24.95290632366073
27	23.48867639424771	24.35942472506263	27.40750686222141	24.744392018468243
28	22.4381184752471	24.823792969543483	30.051813171645524	22.686275383563885
29	23.5949301451035	23.204233482779195	29.622515609636686	23.57832076248062
30	25.670466376417224	23.541108800715765	29.00258631815128	21.785838504715734
31	29.02440421797297	23.11221634560016	25.172647877037324	22.690731559389537
32	29.693640805607142	23.24098246523749	25.066046891701614	21.99932983745375
33	29.464408176446042	23.123675083437554	24.349586414798207	23.062330325318197
34	25.249733931579758	24.15264659226975	27.609828819188643	22.98779065696185
35	25.143422308310655	24.221688381360686	27.91487430979913	22.720015000529532
36	29.437150269772257	23.451117198002937	25.52729002586318	21.584442506361626
37	25.396787733826255	25.974875270481192	26.25451477164414	22.373822224048418
38	26.56864623114589	24.991738712998547	25.87510322991726	22.5645118259383
39	25.156906580614248	24.6307884711208	25.331854886081047	24.880450062183908
40	27.034171923893147	22.57967439822818	26.12557503476685	24.26057864311182
41	23.106429104268148	21.70134478126832	26.81246270846367	28.379763405999864
42	25.413686478515736	23.488502777007753	25.44447460240205	25.653336142074462
43	24.260925877591745	22.681529845671637	27.64854546369982	25.408998813036803
44	23.898470952967667	23.928622480307464	27.260047953081678	24.91285861364319
45	23.076740556234913	25.214605376694433	26.258913075056466	25.449740992014185
46	25.52989428446259	23.77074653677011	26.919642417932575	23.77971676083473
47	24.226028812359697	23.70841794762431	27.852082741346774	24.213470498669224
48	24.512092151401177	22.757342707121026	28.923301111902678	23.80726402957512
49	24.85666450030933	21.782829139223086	28.251113031189178	25.109393329278408
50	23.848932167165625	24.162890009427414	27.688998280610598	24.29917954279636
51	23.148155114271972	25.68192511425461	26.06857070764651	25.101349063826905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1044.0
1	1846.5
2	2649.0
3	9338.5
4	16028.0
5	11189.5
6	6351.0
7	6523.5
8	6696.0
9	7263.5
10	7831.0
11	8097.0
12	8363.0
13	8190.5
14	8018.0
15	8098.0
16	8178.0
17	8046.0
18	7914.0
19	7764.5
20	7615.0
21	7413.0
22	7211.0
23	7597.5
24	7984.0
25	7984.0
26	8775.5
27	9567.0
28	10098.5
29	10630.0
30	12904.5
31	15179.0
32	17403.5
33	19628.0
34	23230.5
35	26833.0
36	28162.5
37	29492.0
38	35207.0
39	40922.0
40	52121.0
41	63320.0
42	75156.0
43	86992.0
44	92435.5
45	97879.0
46	106520.5
47	115162.0
48	120546.5
49	125931.0
50	125964.5
51	125998.0
52	120098.0
53	114198.0
54	109181.5
55	104165.0
56	103533.0
57	102901.0
58	102043.5
59	101186.0
60	98193.0
61	95200.0
62	90411.5
63	85623.0
64	77481.5
65	69340.0
66	62201.0
67	55062.0
68	47542.0
69	40022.0
70	35882.5
71	31743.0
72	27509.0
73	23275.0
74	18952.5
75	11674.5
76	8719.0
77	7263.0
78	5807.0
79	4092.0
80	2377.0
81	1830.5
82	1284.0
83	946.0
84	608.0
85	403.5
86	199.0
87	135.5
88	72.0
89	72.0
90	72.0
91	53.0
92	34.0
93	20.5
94	7.0
95	9.5
96	12.0
97	7.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1727939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.590532954992188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42477989307193	18.50082354555522
2	6.671404823721885	3.1476399070021
3	2.3517259830551334	1.6643540791312055
4	1.3157988074309355	1.241615805153556
5	0.9398834385443483	1.1086175615415912
6	0.6602894220193839	0.9345947621988612
7	0.5436860586876594	0.8978090719248732
8	0.4444996440040488	0.8388786800287849
9	0.37505808050194356	0.7963038007305492
>10	6.346142815368967	39.05251534657763
>50	1.8489849637906546	27.83924932019623
>100	0.07279409091927151	2.479968014053214
>500	0.0034663852187434984	0.5737605511043796
>1k	0.0012379947209798208	0.3746411661186492
>5k	2.4759894419596417E-4	0.5492283886831398
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9403	0.5441743024493341	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2017	0.11672865766673476	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.787241332014614E-5	0.0	0.0	0.06661114773148821	0.0
2	5.787241332014614E-5	0.0	0.0	0.21829474304359123	0.0
3	5.787241332014614E-5	0.0	0.0	0.31465231122163456	0.0
4	5.787241332014614E-5	0.0	0.0	0.5279121543063731	0.0
5	5.787241332014614E-5	0.0	0.0	0.8289065759844532	0.0
6	5.787241332014614E-5	0.0	0.0	1.4616256708136108	0.0
7	5.787241332014614E-5	0.0	0.0	1.7360566547777438	0.0
8	5.787241332014614E-5	0.0	0.0	2.512704441534105	0.0
9	5.787241332014614E-5	0.0	0.0	2.9264343243598296	0.0
10	5.787241332014614E-5	0.0	0.0	3.580161105224201	0.0
11	5.787241332014614E-5	0.0	0.0	4.190020596791901	0.0
12	5.787241332014614E-5	0.0	0.0	4.663764172230617	0.0
13	5.787241332014614E-5	0.0	0.0	4.923148328731512	0.0
14	5.787241332014614E-5	0.0	0.0	5.048731465636229	0.0
15	5.787241332014614E-5	0.0	0.0	5.178307799060036	0.0
16	5.787241332014614E-5	0.0	0.0	5.409739579927301	0.0
17	5.787241332014614E-5	0.0	0.0	5.645974771100137	0.0
18	5.787241332014614E-5	0.0	0.0	5.960627082321772	0.0
19	5.787241332014614E-5	0.0	0.0	6.12238047755158	0.0
20	5.787241332014614E-5	0.0	0.0	6.311623269108458	0.0
21	5.787241332014614E-5	0.0	0.0	6.5218158742872285	0.0
22	5.787241332014614E-5	0.0	0.0	6.731661244986078	0.0
23	5.787241332014614E-5	0.0	0.0	6.964539836186347	0.0
24	5.787241332014614E-5	0.0	0.0	7.157833696675635	0.0
25	5.787241332014614E-5	0.0	0.0	7.316751343652756	0.0
26	5.787241332014614E-5	0.0	0.0	7.473411966510392	0.0
27	5.787241332014614E-5	0.0	0.0	7.641473454792096	0.0
28	5.787241332014614E-5	0.0	0.0	7.799985994875977	0.0
29	5.787241332014614E-5	0.0	0.0	7.981473883047955	0.0
30	5.787241332014614E-5	0.0	0.0	8.258740615264776	0.0
31	1.1574482664029228E-4	0.0	0.0	8.461525551538568	0.0
32	1.1574482664029228E-4	0.0	0.0	8.650594725855484	0.0
33	1.1574482664029228E-4	0.0	0.0	8.844177948411373	0.0
34	1.1574482664029228E-4	0.0	0.0	9.047252246751766	0.0
35	1.1574482664029228E-4	0.0	0.0	9.28626531376397	0.0
36	1.1574482664029228E-4	0.0	0.0	9.479211939773338	0.0
37	1.1574482664029228E-4	0.0	0.0	9.687321138072582	0.0
38	1.1574482664029228E-4	0.0	0.0	9.929980167123956	0.0
39	1.1574482664029228E-4	0.0	0.0	10.314368736396366	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	25	3.8916234E-5	45.0	1
CGTTTTT	5835	0.0	41.413883	1
ATTTACG	60	3.6379788E-12	41.249996	1
GCATATC	330	0.0	38.86364	28
TTCGACG	325	0.0	38.76923	25
TGCGATA	35	6.2501167E-6	38.571426	12
TCGTACA	35	6.2501167E-6	38.571426	34
ATATGCT	1305	0.0	38.448273	34
AGGGTAC	960	0.0	38.437496	6
AGGGCGA	2185	0.0	38.40961	6
CATATGC	1335	0.0	38.258427	33
ATGACGG	195	0.0	38.076923	2
GGGCGAT	4290	0.0	37.86713	7
TTGATCC	1385	0.0	37.851986	17
TAGCATA	1355	0.0	37.52767	30
GCTACGA	90	0.0	37.5	10
GTATACG	30	1.1401597E-4	37.499996	1
TAATGCG	30	1.1401597E-4	37.499996	1
CATACGA	260	0.0	37.21154	18
AGCATAT	1370	0.0	37.11679	31
>>END_MODULE