FastQCFastQC Report
Thu 26 May 2016
SRR1547005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547005_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2077815
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135120.6502985106951292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG551.8189894E-1244.9999961
CGTTTTT86650.041.2088851
CGTAAGG3150.039.9999962
CATATGC10100.039.20792433
CGTTAGG3100.038.467742
CACGACC22250.038.22471627
AGGGCGA25850.038.210836
AACACGT20500.038.0853741
ACACGAC22700.037.5660826
TTAACGG900.037.52
ACGACCA22800.037.40131828
GGGCGAT50950.037.3159947
CGACCAA22800.037.2039529
TCGATAG850.037.0588261
TCAAGCG23000.036.9782617
GCCAGTA11400.036.90789425
TAGGGCG10150.036.7980275
CCAGTAG11450.036.74672326
TACGGGA4850.036.6494874
TAATACG800.036.56251
Produced by FastQC (version 0.11.5)