##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547005_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2077815
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.615438333056602	31.0	31.0	33.0	30.0	34.0
2	32.005988983619815	31.0	31.0	34.0	30.0	34.0
3	32.09600084704365	33.0	31.0	34.0	30.0	34.0
4	35.79517810777187	37.0	35.0	37.0	35.0	37.0
5	35.7501380055491	37.0	35.0	37.0	33.0	37.0
6	35.80470253607756	37.0	35.0	37.0	35.0	37.0
7	36.11247777112014	37.0	35.0	37.0	35.0	37.0
8	36.044443321469906	37.0	35.0	37.0	35.0	37.0
9	37.81060633405765	39.0	38.0	39.0	35.0	39.0
10	37.34207665263751	39.0	37.0	39.0	34.0	39.0
11	37.09503540979346	39.0	37.0	39.0	33.0	39.0
12	36.7382476303232	39.0	35.0	39.0	33.0	39.0
13	36.66129612116574	39.0	35.0	39.0	33.0	39.0
14	37.77210723765109	40.0	36.0	41.0	33.0	41.0
15	37.834751409533574	40.0	36.0	41.0	33.0	41.0
16	37.88696972540866	40.0	36.0	41.0	33.0	41.0
17	37.802338032981766	40.0	36.0	41.0	33.0	41.0
18	37.70677610855635	39.0	36.0	41.0	33.0	41.0
19	37.64345911450249	39.0	36.0	41.0	33.0	41.0
20	37.51681646344838	39.0	35.0	41.0	33.0	41.0
21	37.44567249731088	39.0	35.0	41.0	32.0	41.0
22	37.359754838616524	39.0	35.0	41.0	32.0	41.0
23	37.25276937552188	39.0	35.0	41.0	32.0	41.0
24	37.13812875544743	39.0	35.0	41.0	32.0	41.0
25	37.01642735277202	39.0	35.0	41.0	32.0	41.0
26	36.86996388032621	39.0	35.0	41.0	31.0	41.0
27	36.77137858760284	39.0	35.0	41.0	31.0	41.0
28	36.795962104422195	39.0	35.0	41.0	31.0	41.0
29	36.77765633610307	39.0	35.0	41.0	31.0	41.0
30	36.6691230932494	39.0	35.0	41.0	31.0	41.0
31	36.55208139319429	38.0	35.0	41.0	31.0	41.0
32	36.44355440691303	39.0	35.0	41.0	30.0	41.0
33	36.28796163277289	39.0	35.0	41.0	30.0	41.0
34	36.088745629423215	39.0	35.0	41.0	30.0	41.0
35	35.95336447181294	38.0	35.0	41.0	29.0	41.0
36	35.835309688302374	38.0	35.0	41.0	29.0	41.0
37	35.759489656201346	38.0	35.0	41.0	28.0	41.0
38	35.648864793063865	38.0	35.0	41.0	28.0	41.0
39	35.55368066935699	38.0	35.0	41.0	27.0	41.0
40	35.423078089242786	38.0	35.0	40.0	27.0	41.0
41	35.34611310439091	38.0	35.0	40.0	27.0	41.0
42	35.25859665080866	38.0	34.0	40.0	27.0	41.0
43	35.128942182051816	38.0	34.0	40.0	26.0	41.0
44	34.97744361264117	37.0	34.0	40.0	26.0	41.0
45	34.854897091415744	37.0	34.0	40.0	25.0	41.0
46	34.77266311004589	37.0	34.0	40.0	25.0	41.0
47	34.70067210025917	37.0	34.0	40.0	25.0	41.0
48	34.585323043678095	36.0	34.0	40.0	24.0	41.0
49	34.47710503581888	36.0	34.0	40.0	24.0	41.0
50	34.372650596901074	36.0	34.0	40.0	24.0	41.0
51	34.01318933591297	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	9.0
13	10.0
14	17.0
15	61.0
16	164.0
17	371.0
18	828.0
19	1636.0
20	2857.0
21	4562.0
22	6770.0
23	9997.0
24	14648.0
25	21555.0
26	28910.0
27	34318.0
28	37255.0
29	40665.0
30	45953.0
31	54003.0
32	66700.0
33	86241.0
34	144431.0
35	210197.0
36	164101.0
37	213217.0
38	317674.0
39	570305.0
40	358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.03309967441759	24.242437368100624	26.397104650799037	14.327358306682742
2	33.897820547065066	25.20955908009135	26.819086396045844	14.073533976797743
3	30.102006194006687	25.849221417691183	30.10585639241222	13.942915995889912
4	25.883247546100108	28.57963774445752	30.057103255102113	15.480011454340255
5	23.6465229098837	32.542983855636805	28.59037017251295	15.220123061966536
6	23.016774833178122	40.44883687912543	25.99620274182254	10.538185545873912
7	88.0627967359943	3.239701320858691	7.079119170859774	1.6183827722872344
8	87.71262119101075	2.9630164379408175	7.129989917292925	2.1943724537555074
9	82.3675351270445	4.795422114095817	9.428606492878336	3.4084362659813316
10	42.955123531209466	28.30492608822248	15.397905973342189	13.342044407225858
11	33.26316346739243	26.50134877262894	23.561674162521687	16.67381359745694
12	28.78114750350729	22.834419811195897	28.130993375252366	20.253439310044445
13	24.367280051400147	26.498076103984236	30.117310732668695	19.017333111946925
14	22.414459420111992	27.42063176943087	30.368728688550235	19.796180121906907
15	21.867394354165313	23.667506491193873	34.47708289717804	19.988016257462768
16	25.11691368095812	22.28042438811925	32.9235759680241	19.679085962898526
17	24.25495051291862	22.584060659875878	29.00628785527104	24.15470097193446
18	25.676828784083277	22.094844824972387	30.065236799233812	22.163089591710524
19	27.9244783582754	24.770155187059483	26.31793494608519	20.987431508579927
20	29.920324956745425	24.302741100627344	25.641936361033103	20.134997581594128
21	28.309065051508437	24.84484903612689	26.955527802042052	19.890558110322623
22	26.952880790638247	22.576504645505015	26.74506633169941	23.72554823215734
23	25.290798266448167	26.07513180913604	26.17985720576663	22.45421271864916
24	24.136749421868643	24.83589732483402	30.14604283827001	20.881310415027325
25	25.147811523162556	24.24508437950443	28.510189790717654	22.09691430661536
26	22.95752990521293	26.33391326946817	27.526800990463542	23.181755834855363
27	23.19244013543073	24.971520563669046	28.050716738496927	23.785322562403294
28	22.827393199105792	26.585234970389564	29.168236825703925	21.419135004800715
29	24.117979704641655	24.198063831476816	28.71372090393033	22.9702355599512
30	25.033605012958326	24.494240343822717	28.783265112630335	21.68888953058862
31	26.837567348392422	25.080096158705178	26.14198087895217	21.94035561395023
32	28.029011244985718	24.218132990665676	26.035426638078945	21.717429126269664
33	26.389885528788653	24.346633362450458	25.665181933906535	23.598299174854354
34	24.10849859106802	26.486669891207832	27.634991565659117	21.769839952065027
35	24.512673168689226	26.53999513912451	27.387423808183115	21.55990788400315
36	26.736307130326807	27.18726161857528	24.575768295059955	21.500662956037953
37	25.289113804645748	26.99494420821873	25.369823588721808	22.34611839841372
38	24.586693233035664	27.970199464341146	25.458907554329908	21.98419974829328
39	25.1108496184694	26.417751339748722	24.848410469652013	23.62298857212986
40	26.009437798841574	25.430752978489423	25.98980178697333	22.570007435695672
41	22.345733378573165	25.415448439827415	26.669698697911027	25.569119483688397
42	24.489716360696214	25.65392010357034	25.518874394496144	24.33748914123731
43	23.707452299651315	24.61667665311878	26.998024366943156	24.677846680286745
44	23.662645615706886	25.66936902467255	26.816631894562313	23.85135346505825
45	23.15105050257121	25.752581437712212	26.00053421502877	25.09583384468781
46	24.33195448102935	25.91188339674129	26.403168713287755	23.352993408941604
47	23.57433168977989	25.7472874149046	27.859458132701903	22.81892276261361
48	24.567394113527914	24.788395502005713	27.879767929291106	22.764442455175267
49	24.3321951184297	24.798694782740522	27.35084692333052	23.518263175499264
50	23.244947216186233	25.96645995913977	27.61054280578396	23.178050018890037
51	23.570962766175043	25.93662092149686	26.30484427150637	24.187572040821728
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1184.0
1	1891.0
2	2598.0
3	12936.5
4	23275.0
5	16209.5
6	9144.0
7	9125.5
8	9107.0
9	9464.0
10	9821.0
11	10168.5
12	10516.0
13	10381.5
14	10247.0
15	9913.0
16	9579.0
17	9525.5
18	9472.0
19	9458.5
20	9445.0
21	9183.0
22	8921.0
23	9711.5
24	10502.0
25	11001.0
26	13343.5
27	15187.0
28	17094.5
29	19002.0
30	22639.0
31	26276.0
32	29849.5
33	33423.0
34	38371.5
35	43320.0
36	47459.5
37	51599.0
38	58174.5
39	64750.0
40	74182.5
41	83615.0
42	93520.5
43	103426.0
44	109337.0
45	115248.0
46	120832.0
47	126416.0
48	134573.0
49	142730.0
50	151556.0
51	160382.0
52	158673.0
53	156964.0
54	147200.0
55	137436.0
56	126998.5
57	116561.0
58	113283.0
59	110005.0
60	104577.5
61	99150.0
62	93756.0
63	88362.0
64	79601.0
65	70840.0
66	62187.0
67	53534.0
68	46422.5
69	39311.0
70	33260.5
71	27210.0
72	24249.5
73	21289.0
74	18844.0
75	13087.5
76	9776.0
77	7449.0
78	5122.0
79	3969.5
80	2817.0
81	2097.5
82	1378.0
83	979.0
84	580.0
85	415.0
86	250.0
87	172.5
88	95.0
89	65.0
90	35.0
91	22.5
92	10.0
93	7.5
94	5.0
95	2.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2077815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.47846216244133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52031066307524	17.731007563988264
2	6.274163869313094	2.9461543801324797
3	2.4136524313686434	1.7000654184951964
4	1.4859944237684757	1.3955545540818783
5	1.129226642572697	1.325625250023186
6	0.864542769684728	1.2178880823513067
7	0.7173944382508658	1.1790322721813162
8	0.6446187140011683	1.2107724868702414
9	0.5543841427455729	1.1714478407018463
>10	9.48424794707438	54.7940551980286
>50	0.8596797911691474	12.231682707521534
>100	0.0490914040361534	2.0101723821895425
>500	0.0022784917182438755	0.3775322032872425
>1k	2.071356107494432E-4	0.05189061714639801
>5k	0.0	0.0
>10k+	2.071356107494432E-4	0.657119043001059
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13512	0.6502985106951292	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03855011153543506	0.0
2	0.0	0.0	0.0	0.11945240553177255	0.0
3	0.0	0.0	0.0	0.19462752939987438	0.0
4	0.0	0.0	0.0	0.3268818446300561	0.0
5	0.0	0.0	0.0	0.5537547856762994	0.0
6	0.0	0.0	0.0	1.0856596954011786	0.0
7	0.0	0.0	0.0	1.3430454588112994	0.0
8	0.0	0.0	0.0	1.9633124219432432	0.0
9	0.0	0.0	0.0	2.338177364202299	0.0
10	0.0	0.0	0.0	2.8480880155355504	0.0
11	0.0	0.0	0.0	3.238642516297168	0.0
12	0.0	0.0	0.0	3.5403536888510287	0.0
13	0.0	0.0	0.0	3.715008314022182	0.0
14	0.0	0.0	0.0	3.8149209626458562	0.0
15	0.0	0.0	0.0	3.9073738518588037	0.0
16	0.0	0.0	0.0	4.054355175990163	0.0
17	0.0	0.0	0.0	4.211347015975917	0.0
18	0.0	0.0	0.0	4.416081316190325	0.0
19	0.0	0.0	0.0	4.5348118095210594	0.0
20	0.0	0.0	0.0	4.655756166934977	0.0
21	0.0	0.0	0.0	4.802833746026475	0.0
22	0.0	0.0	0.0	4.95732295704863	0.0
23	0.0	0.0	0.0	5.131544434899161	0.0
24	0.0	0.0	0.0	5.278910778871074	0.0
25	0.0	0.0	0.0	5.407796170496411	0.0
26	0.0	0.0	0.0	5.530280607272544	0.0
27	0.0	0.0	0.0	5.662294285102379	0.0
28	0.0	0.0	0.0	5.799794495660104	0.0
29	0.0	4.8127480069207316E-5	0.0	5.9446100831883495	0.0
30	0.0	4.8127480069207316E-5	0.0	6.149296255922688	0.0
31	0.0	4.8127480069207316E-5	0.0	6.307587537870311	0.0
32	0.0	4.8127480069207316E-5	0.0	6.49581411242098	0.0
33	0.0	4.8127480069207316E-5	0.0	6.660458221737739	0.0
34	0.0	4.8127480069207316E-5	0.0	6.826690537896781	0.0
35	4.8127480069207316E-5	4.8127480069207316E-5	0.0	7.0080348827975545	0.0
36	4.8127480069207316E-5	4.8127480069207316E-5	0.0	7.166181782304969	0.0
37	4.8127480069207316E-5	4.8127480069207316E-5	0.0	7.337852503711832	0.0
38	4.8127480069207316E-5	4.8127480069207316E-5	0.0	7.5369558887581425	0.0
39	4.8127480069207316E-5	4.8127480069207316E-5	0.0	7.787315040078159	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAG	55	1.8189894E-12	44.999996	1
CGTTTTT	8665	0.0	41.208885	1
CGTAAGG	315	0.0	39.999996	2
CATATGC	1010	0.0	39.207924	33
CGTTAGG	310	0.0	38.46774	2
CACGACC	2225	0.0	38.224716	27
AGGGCGA	2585	0.0	38.21083	6
AACACGT	2050	0.0	38.08537	41
ACACGAC	2270	0.0	37.56608	26
TTAACGG	90	0.0	37.5	2
ACGACCA	2280	0.0	37.401318	28
GGGCGAT	5095	0.0	37.315994	7
CGACCAA	2280	0.0	37.20395	29
TCGATAG	85	0.0	37.058826	1
TCAAGCG	2300	0.0	36.97826	17
GCCAGTA	1140	0.0	36.907894	25
TAGGGCG	1015	0.0	36.798027	5
CCAGTAG	1145	0.0	36.746723	26
TACGGGA	485	0.0	36.649487	4
TAATACG	80	0.0	36.5625	1
>>END_MODULE