FastQCFastQC Report
Thu 26 May 2016
SRR1547003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547003_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2108095
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96700.458707980427827No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC24770.11749944855426345No Hit
GAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT24480.1161237989749039No Hit
AAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT23520.1115699245052998No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT22400.1062570709574284No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT22120.10492885757046053No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22110.10488142137806881No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT21960.1041698784921932Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCA207.034626E-445.00000418
TAATTCG207.034626E-445.00000421
TCGAATC207.034626E-445.00000421
GTCGAAC302.1663673E-644.99999643
CGAAACG302.1663673E-644.99999613
CGAATAT1050.042.85714314
CGTTTTT63850.040.9827731
ATCGTAG1050.040.7142871
GCGTAAG1850.040.1351361
CGTTAGG2650.039.9056632
TATACGG1600.039.3750042
TAGCATA7100.038.66197230
CAAATCG356.2507697E-638.5714330
GGGCGAT44900.038.4354137
CGCCAGT1350.038.33333226
ATAGACG1000.038.251
ATTGGGA11250.038.24
CTATGCG659.094947E-1238.076921
GCGTTAG1600.037.9687541
TGCTACG950.037.8947371