##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547003_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2108095
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.648649135831164	31.0	31.0	33.0	30.0	34.0
2	32.02003135532317	33.0	31.0	34.0	30.0	34.0
3	32.11644968561664	33.0	31.0	34.0	30.0	34.0
4	35.7841629528081	37.0	35.0	37.0	35.0	37.0
5	35.7371195320894	37.0	35.0	37.0	33.0	37.0
6	35.78503198385272	37.0	35.0	37.0	33.0	37.0
7	36.1164131597485	37.0	35.0	37.0	35.0	37.0
8	36.04103040897113	37.0	35.0	37.0	35.0	37.0
9	37.81412175447501	39.0	38.0	39.0	35.0	39.0
10	37.38062089232221	39.0	37.0	39.0	34.0	39.0
11	37.120975572732725	39.0	37.0	39.0	33.0	39.0
12	36.52995476959055	39.0	35.0	39.0	33.0	39.0
13	36.297661158534126	39.0	35.0	39.0	32.0	39.0
14	37.29907902632471	40.0	35.0	41.0	32.0	41.0
15	37.47745002004179	40.0	35.0	41.0	33.0	41.0
16	37.59420187420396	40.0	35.0	41.0	33.0	41.0
17	37.55175264871839	39.0	35.0	41.0	33.0	41.0
18	37.47373861234907	39.0	35.0	41.0	33.0	41.0
19	37.42360282624834	39.0	35.0	41.0	33.0	41.0
20	37.26285437800479	39.0	35.0	41.0	32.0	41.0
21	37.1886433011795	39.0	35.0	41.0	32.0	41.0
22	37.122011104812636	39.0	35.0	41.0	32.0	41.0
23	37.03934500105546	39.0	35.0	41.0	32.0	41.0
24	36.94032621869508	38.0	35.0	41.0	32.0	41.0
25	36.810839169961504	38.0	35.0	41.0	32.0	41.0
26	36.65471242994267	38.0	35.0	41.0	31.0	41.0
27	36.559327734281425	38.0	35.0	41.0	31.0	41.0
28	36.61121154407178	38.0	35.0	41.0	31.0	41.0
29	36.61495188784187	38.0	35.0	41.0	31.0	41.0
30	36.53292142906273	38.0	35.0	41.0	31.0	41.0
31	36.400891800416964	38.0	35.0	41.0	31.0	41.0
32	36.275966215943775	38.0	35.0	41.0	31.0	41.0
33	36.133163828005856	38.0	35.0	41.0	30.0	41.0
34	35.95229247258781	38.0	35.0	41.0	30.0	41.0
35	35.84830759524594	38.0	35.0	41.0	30.0	41.0
36	35.70736755222132	38.0	35.0	41.0	29.0	41.0
37	35.59937573970812	38.0	35.0	40.0	29.0	41.0
38	35.481468339899294	38.0	35.0	40.0	29.0	41.0
39	35.36252777982017	38.0	35.0	40.0	28.0	41.0
40	35.21355963559517	37.0	34.0	40.0	27.0	41.0
41	35.13878074754695	37.0	34.0	40.0	27.0	41.0
42	35.04687312478802	37.0	34.0	40.0	27.0	41.0
43	34.919562922923305	37.0	34.0	40.0	26.0	41.0
44	34.776061325509524	37.0	34.0	40.0	26.0	41.0
45	34.69611378993831	36.0	34.0	40.0	26.0	41.0
46	34.649929913025744	36.0	34.0	40.0	25.0	41.0
47	34.585966002480916	36.0	34.0	40.0	25.0	41.0
48	34.49857003598035	36.0	34.0	40.0	25.0	41.0
49	34.42381439166641	36.0	34.0	40.0	25.0	41.0
50	34.291241144255835	35.0	34.0	40.0	24.0	41.0
51	33.942589399434084	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	4.0
13	3.0
14	17.0
15	53.0
16	150.0
17	409.0
18	867.0
19	1764.0
20	3046.0
21	4803.0
22	7144.0
23	10773.0
24	16320.0
25	22361.0
26	28212.0
27	33111.0
28	36142.0
29	40862.0
30	47112.0
31	56783.0
32	70530.0
33	92380.0
34	164299.0
35	277615.0
36	156420.0
37	198642.0
38	288706.0
39	549098.0
40	466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.94643505155128	24.182781136523733	27.346870041435512	12.52391377048947
2	32.328381785450844	26.610091101207487	26.972693355849714	14.088833757491953
3	30.672479181441066	25.773221795032956	29.37675958626153	14.17753943726445
4	26.004378360557755	29.487001297379862	29.161921070919476	15.346699271142905
5	23.88815494557883	32.909996940365595	28.054618031919816	15.147230082135769
6	22.095825852250492	41.343060915186456	25.800592478042972	10.760520754520076
7	86.20223471902358	4.567820710167236	7.285629917057817	1.9443146537513727
8	85.29667780626585	3.9977325500036764	6.9438995870679445	3.7616900566625318
9	80.00199232008045	5.657619794174361	10.335207853536012	4.005180032209175
10	47.526985263946834	23.153320889238863	15.365958365253936	13.953735481560367
11	38.77097569132321	24.73697817223607	21.207583149715738	15.28446298672498
12	33.54369703452643	22.16375447975542	26.016189972463287	18.27635851325486
13	23.754527191611384	31.245935311264432	28.51332601234764	16.48621148477654
14	19.331766357777994	33.42188089246452	30.00320194298644	17.243150806771045
15	18.416437589387574	23.806991620396616	40.224325753820395	17.55224503639542
16	21.151086644577212	21.238226930000785	38.340492245368445	19.270194180053554
17	21.430960179688295	22.112333647202806	28.204848453224358	28.251857719884537
18	23.171109461385754	23.12438481187992	31.996613055863232	21.707892670871097
19	27.772040633842405	25.131030622433997	26.656863186905717	20.440065556817885
20	28.629734428476894	25.266603260289504	25.007174724099247	21.096487587134355
21	25.177186037631134	27.63096539766946	27.637891081758646	19.55395748294076
22	24.67507394116489	24.21461082161857	26.320635455233276	24.78967978198326
23	22.956270946043702	28.598663722460326	24.956085944893374	23.488979386602598
24	22.904707804913915	24.977479667662035	31.879018734924188	20.238793792499862
25	22.286614218049948	24.603635035422975	30.649188010976737	22.460562735550344
26	20.04909645912542	29.36153256850379	27.695383746937402	22.89398722543339
27	21.440210237204678	28.42533187546102	27.907328654543555	22.227129232790745
28	19.541102274802608	28.028528126104373	31.704263802153125	20.72610579693989
29	20.554481652866688	26.54045477077646	30.572673432648905	22.332390143707944
30	21.79213934855877	27.771708580495662	29.14052734815082	21.295624722794752
31	26.346772797241115	26.709280179498553	25.007079851714465	21.936867171545874
32	27.657339920639252	26.257450446967525	25.71406886312049	20.371140769272735
33	26.121830372919625	27.289140195294802	24.642959638915706	21.946069792869867
34	21.42944222153176	27.528503222103367	27.721141599406096	23.320912956958768
35	21.881034773100833	26.909698092353523	28.247493590184504	22.96177354436114
36	28.30773755452197	26.255885052618595	25.029090244984214	20.407287147875213
37	22.734079820880936	28.971939120390683	26.477364634895483	21.81661642383289
38	24.005464649363525	29.527084879950856	24.13155004874069	22.33590042194493
39	22.701965518631752	27.705629964494012	26.125672704503355	23.466731812370885
40	25.50459063751871	24.32532689466082	26.2329733716934	23.93710909612707
41	20.45562462792237	23.81766476368475	27.75501104077378	27.97169956761911
42	23.825823788776123	24.849828873935948	26.31612901695607	25.008218320331864
43	22.358527485715776	24.309151153055247	28.229752454230006	25.10256890699897
44	22.020449742540066	26.401846216607883	27.624703820273755	23.953000220578293
45	20.864145116799765	28.303373424821938	25.5120855559166	25.3203959024617
46	23.483998586401466	27.03791812038831	26.657195240252456	22.820888052957766
47	22.46241274705362	25.2334453618077	28.015293428427086	24.288848462711595
48	23.23813680123524	24.039618707885555	29.675797343098864	23.04644714778034
49	23.678534411399866	23.156499114129105	29.310870715029445	23.85409575944158
50	21.620183151138825	27.17306383251229	27.608290897706222	23.59846211864266
51	20.66244642675022	27.88816443281731	26.234633638427113	25.214755502005364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1797.0
1	2738.5
2	3680.0
3	10213.5
4	16747.0
5	12096.0
6	7445.0
7	7921.5
8	8398.0
9	9259.5
10	10121.0
11	10425.5
12	10730.0
13	10826.0
14	10922.0
15	10928.0
16	10934.0
17	11049.5
18	11165.0
19	11292.0
20	11419.0
21	11288.5
22	11158.0
23	10966.5
24	10775.0
25	11644.5
26	14050.0
27	15586.0
28	16463.0
29	17340.0
30	21370.0
31	25400.0
32	28284.5
33	31169.0
34	34393.5
35	37618.0
36	43238.5
37	48859.0
38	55194.5
39	61530.0
40	82042.0
41	102554.0
42	128759.5
43	154965.0
44	168904.0
45	182843.0
46	186983.5
47	191124.0
48	183456.0
49	175788.0
50	161040.0
51	146292.0
52	136208.5
53	126125.0
54	116553.5
55	106982.0
56	102773.5
57	98565.0
58	97500.5
59	96436.0
60	90213.5
61	83991.0
62	77652.0
63	71313.0
64	63550.5
65	55788.0
66	49739.0
67	43690.0
68	37316.0
69	30942.0
70	27212.0
71	23482.0
72	19963.0
73	16444.0
74	13413.5
75	8789.0
76	7195.0
77	5409.0
78	3623.0
79	2891.5
80	2160.0
81	1821.0
82	1482.0
83	945.0
84	408.0
85	260.0
86	112.0
87	86.5
88	61.0
89	43.5
90	26.0
91	17.0
92	8.0
93	5.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2108095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.658547561559683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0481948936625	17.12069088759828
2	7.928218457771406	3.434273930921547
3	2.8075048940554157	1.8241943483163239
4	1.3135485541371479	1.1379821533678949
5	0.8645350247043354	0.9362286475596513
6	0.5958400373963533	0.7743017873418249
7	0.4437032665841436	0.6726977811772449
8	0.35187793168116244	0.6096931935343765
9	0.304983337145821	0.594494650175036
>10	3.6930389928341523	19.758855563899402
>50	1.8862764292069207	29.987473398642422
>100	0.7554147995089774	20.940065816943903
>500	0.0037637897516219925	0.5694287380871693
>1k	0.002878192163005053	1.1759242900304552
>5k	2.2139939715423485E-4	0.4636948124044571
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9670	0.458707980427827	No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	2477	0.11749944855426345	No Hit
GAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	2448	0.1161237989749039	No Hit
AAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	2352	0.1115699245052998	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	2240	0.1062570709574284	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	2212	0.10492885757046053	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2211	0.10488142137806881	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	2196	0.1041698784921932	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.48723847834182E-5	0.0	0.0	0.1119968502368252	0.0
2	9.48723847834182E-5	0.0	0.0	0.4078089459915232	0.0
3	9.48723847834182E-5	0.0	0.0	0.6016806642964383	0.0
4	9.48723847834182E-5	0.0	0.0	1.0886131791973321	0.0
5	9.48723847834182E-5	0.0	0.0	1.7214594218951234	0.0
6	9.48723847834182E-5	0.0	0.0	2.754856873148506	0.0
7	9.48723847834182E-5	0.0	0.0	3.1835377437923813	0.0
8	9.48723847834182E-5	0.0	0.0	4.345819329774038	0.0
9	9.48723847834182E-5	0.0	0.0	4.839677528764121	0.0
10	9.48723847834182E-5	0.0	0.0	5.647800502349277	0.0
11	9.48723847834182E-5	0.0	0.0	6.457299125513793	0.0
12	9.48723847834182E-5	0.0	0.0	7.087204324283299	0.0
13	9.48723847834182E-5	0.0	0.0	7.372912511058562	0.0
14	9.48723847834182E-5	0.0	0.0	7.498997910435725	0.0
15	9.48723847834182E-5	0.0	0.0	7.654873238634881	0.0
16	1.423085771751273E-4	0.0	0.0	7.985788116759444	0.0
17	1.423085771751273E-4	0.0	0.0	8.314141440494854	0.0
18	1.423085771751273E-4	0.0	0.0	8.778826381164036	0.0
19	1.423085771751273E-4	0.0	0.0	9.001159814903977	0.0
20	1.423085771751273E-4	0.0	0.0	9.236680510128814	0.0
21	1.423085771751273E-4	0.0	0.0	9.531781062997636	0.0
22	1.423085771751273E-4	0.0	0.0	9.82194825185772	0.0
23	1.423085771751273E-4	0.0	0.0	10.132228386291889	0.0
24	1.423085771751273E-4	0.0	0.0	10.387150484204934	0.0
25	1.423085771751273E-4	0.0	0.0	10.596438965037155	0.0
26	1.423085771751273E-4	0.0	0.0	10.80197998667043	0.0
27	1.423085771751273E-4	0.0	0.0	11.0104146160396	0.0
28	1.423085771751273E-4	0.0	0.0	11.218517192062027	0.0
29	1.423085771751273E-4	0.0	0.0	11.457168675984716	0.0
30	1.423085771751273E-4	0.0	0.0	11.763084680718848	0.0
31	1.423085771751273E-4	0.0	0.0	12.025501697029783	0.0
32	1.423085771751273E-4	0.0	0.0	12.255140304398047	0.0
33	1.423085771751273E-4	0.0	0.0	12.496637959864238	0.0
34	1.423085771751273E-4	0.0	0.0	12.744112575571783	0.0
35	1.423085771751273E-4	0.0	0.0	13.03816953220799	0.0
36	1.423085771751273E-4	0.0	0.0	13.276014600860018	0.0
37	1.423085771751273E-4	0.0	0.0	13.514144286666397	0.0
38	1.423085771751273E-4	0.0	0.0	13.800896069674279	0.0
39	1.423085771751273E-4	0.0	0.0	14.203534470695105	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTCA	20	7.034626E-4	45.000004	18
TAATTCG	20	7.034626E-4	45.000004	21
TCGAATC	20	7.034626E-4	45.000004	21
GTCGAAC	30	2.1663673E-6	44.999996	43
CGAAACG	30	2.1663673E-6	44.999996	13
CGAATAT	105	0.0	42.857143	14
CGTTTTT	6385	0.0	40.982773	1
ATCGTAG	105	0.0	40.714287	1
GCGTAAG	185	0.0	40.135136	1
CGTTAGG	265	0.0	39.905663	2
TATACGG	160	0.0	39.375004	2
TAGCATA	710	0.0	38.661972	30
CAAATCG	35	6.2507697E-6	38.57143	30
GGGCGAT	4490	0.0	38.435413	7
CGCCAGT	135	0.0	38.333332	26
ATAGACG	100	0.0	38.25	1
ATTGGGA	1125	0.0	38.2	4
CTATGCG	65	9.094947E-12	38.07692	1
GCGTTAG	160	0.0	37.968754	1
TGCTACG	95	0.0	37.894737	1
>>END_MODULE