FastQCFastQC Report
Thu 26 May 2016
SRR1547002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547002_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2394660
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146920.613531774865743No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85500.040.6578941
TAATCCG403.460318E-739.3750041
CTAATCC13500.038.83333211
TACGGGA7350.037.9591874
GGGCGAT51400.037.689697
GACTAAT14150.037.52659
TCGTTAG1500.037.5000041
CATATGC13750.036.8181833
AGGGCGA28600.036.818186
ACTAATC14250.036.78947410
TAGGGAC19950.036.766925
CGTAAGG3250.036.6923072
ATAGGGA24300.036.481484
ACGACCA6050.036.4462828
GGCGATA10700.036.3785068
CGTTAGG4350.036.2068942
GCGATAA2550.036.176479
CACGACC6100.036.1475427
GGTACCT15250.036.1475378
CCCTACA13850.036.06498327
Produced by FastQC (version 0.11.5)