##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547002_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2394660
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61405794559562	31.0	31.0	33.0	30.0	34.0
2	32.01184009420962	31.0	31.0	34.0	30.0	34.0
3	32.11390719350555	33.0	31.0	34.0	30.0	34.0
4	35.797427192169245	37.0	35.0	37.0	35.0	37.0
5	35.747530338336134	37.0	35.0	37.0	33.0	37.0
6	35.79751655767416	37.0	35.0	37.0	35.0	37.0
7	36.119613640349776	37.0	35.0	37.0	35.0	37.0
8	36.0514578269984	37.0	35.0	37.0	35.0	37.0
9	37.81176659734576	39.0	38.0	39.0	35.0	39.0
10	37.351739704175124	39.0	37.0	39.0	34.0	39.0
11	37.080502868883265	39.0	37.0	39.0	33.0	39.0
12	36.72267127692449	39.0	35.0	39.0	33.0	39.0
13	36.63471432270134	39.0	35.0	39.0	33.0	39.0
14	37.747899910634494	40.0	36.0	41.0	33.0	41.0
15	37.82507412325758	40.0	36.0	41.0	33.0	41.0
16	37.88606232199978	40.0	36.0	41.0	33.0	41.0
17	37.83706455196145	40.0	36.0	41.0	33.0	41.0
18	37.729491869409436	39.0	36.0	41.0	33.0	41.0
19	37.67489748022684	39.0	36.0	41.0	33.0	41.0
20	37.54173285560372	39.0	35.0	41.0	33.0	41.0
21	37.473793356885736	39.0	35.0	41.0	33.0	41.0
22	37.40097216306282	39.0	35.0	41.0	32.0	41.0
23	37.29817218310741	39.0	35.0	41.0	32.0	41.0
24	37.187204446560266	39.0	35.0	41.0	32.0	41.0
25	37.05310524249789	39.0	35.0	41.0	32.0	41.0
26	36.90371660277451	39.0	35.0	41.0	31.0	41.0
27	36.817459263528015	39.0	35.0	41.0	31.0	41.0
28	36.83049242898783	39.0	35.0	41.0	31.0	41.0
29	36.814366966500465	39.0	35.0	41.0	31.0	41.0
30	36.72862118213024	39.0	35.0	41.0	31.0	41.0
31	36.62185905305972	39.0	35.0	41.0	31.0	41.0
32	36.54685675628273	39.0	35.0	41.0	31.0	41.0
33	36.394766689216844	39.0	35.0	41.0	30.0	41.0
34	36.213400232183275	39.0	35.0	41.0	30.0	41.0
35	36.09137706396733	39.0	35.0	41.0	30.0	41.0
36	35.98773896920648	38.0	35.0	41.0	29.0	41.0
37	35.915911235833065	38.0	35.0	41.0	29.0	41.0
38	35.824692440680515	38.0	35.0	41.0	29.0	41.0
39	35.7355624598064	38.0	35.0	41.0	29.0	41.0
40	35.60183157525494	38.0	35.0	41.0	28.0	41.0
41	35.51856631003984	38.0	35.0	40.0	28.0	41.0
42	35.413562676956225	38.0	35.0	40.0	27.0	41.0
43	35.28519622827458	38.0	34.0	40.0	27.0	41.0
44	35.1300802619161	37.0	34.0	40.0	26.0	41.0
45	35.01569241562476	37.0	34.0	40.0	26.0	41.0
46	34.95639297436797	37.0	34.0	40.0	26.0	41.0
47	34.88550107322125	37.0	34.0	40.0	26.0	41.0
48	34.78069078700108	37.0	34.0	40.0	26.0	41.0
49	34.683768468175025	36.0	34.0	40.0	26.0	41.0
50	34.576111431267904	36.0	34.0	40.0	26.0	41.0
51	34.196999991648084	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	1.0
12	6.0
13	7.0
14	29.0
15	59.0
16	160.0
17	403.0
18	935.0
19	1741.0
20	3127.0
21	5036.0
22	7499.0
23	11056.0
24	16154.0
25	23330.0
26	31191.0
27	37056.0
28	40571.0
29	45446.0
30	52685.0
31	62675.0
32	75967.0
33	99249.0
34	165972.0
35	245193.0
36	184366.0
37	243069.0
38	360057.0
39	681135.0
40	481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.415557949771575	24.428979479341535	27.90993293411173	14.245529636775158
2	32.21651507938497	26.106754194749985	28.17264246281309	13.504088263051958
3	29.597228834156	25.498859963418607	30.927772627429363	13.976138574996034
4	25.995590188168677	28.636925492554266	29.97836853666074	15.389115782616322
5	23.737106729139	32.766864607084095	28.583431468350412	14.91259719542649
6	22.379628005645895	41.948627362548336	25.230304093274203	10.441440538531566
7	88.34018190473805	3.4575680889980207	6.630544628465001	1.5717053777989358
8	88.05817944927463	3.061645494558727	6.536418531231991	2.3437565249346464
9	82.15567136879557	5.314324371727093	9.122380630235607	3.4076236292417295
10	44.63372670859329	27.384680915035954	14.841898223547393	13.139694152823363
11	32.54846199460466	24.92637785739938	24.66838716143419	17.85677298656177
12	29.245446117611685	21.4782474338737	28.958014916522597	20.318291531992017
13	24.143302180685357	26.2712451872082	30.11955768251025	19.465894949596183
14	21.68788053418857	27.74886622735587	30.3655633785172	20.197689859938365
15	20.661972889679536	23.142659083126624	35.79226278469595	20.40310524249789
16	23.682693994136955	21.43682192879156	33.86230195518362	21.018182121887868
17	23.78245763490433	21.577050604261146	28.77251885445115	25.86797290638337
18	24.12476092639456	22.54754328380647	31.156949211996693	22.170746577802277
19	26.896636683286978	24.514461343155187	27.05198232734501	21.536919646212823
20	28.32673531941904	23.78538080562585	26.70604595224374	21.181837922711367
21	26.205766163046114	24.70964562818939	28.228475023594164	20.85611318517034
22	26.085916163463708	23.179658072544747	27.036155445867056	23.698270318124493
23	24.409978869651642	25.36276548654089	26.36491192904212	23.862343714765352
24	23.67200354121253	23.89758880175056	30.753509892844917	21.67689776419199
25	24.096656727886216	24.298355507671236	28.706747513216907	22.898240251225644
26	22.476510235273484	26.431852538564975	27.335070531933553	23.756566694227992
27	22.43328906817669	25.42373447587549	28.168424745057752	23.97455171089006
28	22.154669138833903	25.744782140262085	30.163321724169613	21.937226996734402
29	23.069830372578988	23.80312862786366	29.9875974042244	23.13944359533295
30	24.274552546081697	24.786608537328892	29.44835592526705	21.49048299132236
31	26.32544912430157	24.55083393884727	26.748933042686645	22.374783894164516
32	27.5062012978878	24.136704166771068	26.790442066932258	21.566652468408876
33	26.316262016319648	24.365170838448883	26.744297729113946	22.574269416117527
34	23.800163697560407	24.557724269833713	28.324730859495713	23.317381173110167
35	23.77226829696074	24.899317648434433	28.364110145072786	22.964303909532042
36	26.50894072644968	24.826029582487703	26.885403355799987	21.779626335262627
37	24.35193305103856	26.160415257280782	27.0544043830857	22.433247308594957
38	24.283530856154943	26.175323427960546	26.583314541521553	22.95783117436296
39	23.505842165484868	25.537529336106168	26.314215796814576	24.64241270159438
40	24.782056742919664	23.739194708225803	27.37954448648242	24.099204062372113
41	21.825144279354898	23.745458645486206	27.672863788596292	26.7565332865626
42	23.816575213182663	24.707265332030435	26.274293636674933	25.20186581811197
43	23.267812549589504	23.91496078775275	28.326108925693	24.491117736964746
44	23.03425120893989	25.380429789615228	27.402554016018975	24.182764985425905
45	22.211378650831435	26.162753793858002	27.099838808014496	24.526028747296067
46	23.82776678108792	25.112876149432488	27.78636633175482	23.272990737724772
47	23.107748072795303	24.78472935615077	28.348617340248722	23.758905230805208
48	23.33796864690603	24.267244619277896	28.68432261782466	23.710464115991414
49	23.66624071893296	23.717354446977858	28.418940475892196	24.19746435819699
50	22.518687412826875	25.322300451838675	28.712552095078216	23.446460040256238
51	21.925158477612687	25.59874888293119	27.755297202943215	24.72079543651291
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1325.0
1	2309.5
2	3294.0
3	13820.5
4	24347.0
5	16842.5
6	9338.0
7	9449.0
8	9560.0
9	9975.0
10	10390.0
11	10603.0
12	10816.0
13	10679.5
14	10543.0
15	10466.0
16	10389.0
17	10047.5
18	9706.0
19	9821.5
20	9937.0
21	9651.5
22	9366.0
23	10966.5
24	12567.0
25	12981.0
26	16295.0
27	19195.0
28	21342.0
29	23489.0
30	27287.0
31	31085.0
32	37048.5
33	43012.0
34	48742.5
35	54473.0
36	59229.5
37	63986.0
38	72425.5
39	80865.0
40	90368.5
41	99872.0
42	110833.5
43	121795.0
44	132369.0
45	142943.0
46	146444.0
47	149945.0
48	160757.0
49	171569.0
50	180021.5
51	188474.0
52	181916.5
53	175359.0
54	162095.0
55	148831.0
56	141988.5
57	135146.0
58	129898.0
59	124650.0
60	117346.0
61	110042.0
62	103488.5
63	96935.0
64	85498.0
65	74061.0
66	66712.0
67	59363.0
68	51302.5
69	43242.0
70	37968.0
71	32694.0
72	28307.5
73	23921.0
74	19287.5
75	11758.5
76	8863.0
77	7213.5
78	5564.0
79	4279.5
80	2995.0
81	2236.5
82	1478.0
83	1091.0
84	704.0
85	484.0
86	264.0
87	200.5
88	137.0
89	91.0
90	45.0
91	36.0
92	27.0
93	15.5
94	4.0
95	3.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2394660.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.536157188298827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53943644160034	17.474409279400117
2	6.940624016462326	3.1282998763975387
3	2.433121558902025	1.6449962972896415
4	1.3117134706239966	1.1824392383996554
5	0.9038171941425307	1.0184283178341635
6	0.6454668499383829	0.8727805434028492
7	0.5573810133275166	0.8792858291085537
8	0.48476850806462857	0.8739855438145263
9	0.3948383638902847	0.800832548934197
>10	6.69676264380165	38.57214161605773
>50	2.000115404951152	29.554702862839406
>100	0.08840351067467597	2.7304164043330577
>500	0.002803439700276601	0.4548028174804548
>1k	5.606879400553203E-4	0.19366395209993809
>5k	0.0	0.0
>10k+	1.868959800184401E-4	0.6188148726081536
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14692	0.613531774865743	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1759581736029334E-5	0.0	0.0	0.057210626978360185	0.0
2	4.1759581736029334E-5	0.0	0.0	0.16933510393959894	0.0
3	4.1759581736029334E-5	0.0	0.0	0.2554016018975554	0.0
4	4.1759581736029334E-5	0.0	0.0	0.4147144062205073	0.0
5	4.1759581736029334E-5	0.0	0.0	0.6850241787978252	0.0
6	4.1759581736029334E-5	0.0	0.0	1.2651065286930085	0.0
7	4.1759581736029334E-5	0.0	0.0	1.5339547159095654	0.0
8	4.1759581736029334E-5	0.0	0.0	2.250757936408509	0.0
9	4.1759581736029334E-5	0.0	0.0	2.6769144680246884	0.0
10	4.1759581736029334E-5	0.0	0.0	3.311158995431502	0.0
11	4.1759581736029334E-5	0.0	0.0	3.858418314082166	0.0
12	4.1759581736029334E-5	0.0	0.0	4.296768643565266	0.0
13	4.1759581736029334E-5	0.0	0.0	4.531039897104391	0.0
14	4.1759581736029334E-5	0.0	0.0	4.64884367718173	0.0
15	4.1759581736029334E-5	0.0	0.0	4.766480418932124	0.0
16	4.1759581736029334E-5	0.0	0.0	4.974109059323662	0.0
17	8.351916347205867E-5	0.0	0.0	5.204329633434392	0.0
18	8.351916347205867E-5	0.0	0.0	5.491176200379177	0.0
19	8.351916347205867E-5	0.0	0.0	5.655667192837397	0.0
20	8.351916347205867E-5	0.0	0.0	5.834189404758922	0.0
21	8.351916347205867E-5	0.0	0.0	6.034468358764919	0.0
22	8.351916347205867E-5	0.0	0.0	6.255543584475458	0.0
23	8.351916347205867E-5	0.0	0.0	6.4862235139852835	0.0
24	8.351916347205867E-5	0.0	0.0	6.680238530730876	0.0
25	1.25278745208088E-4	0.0	0.0	6.84773621307409	0.0
26	1.25278745208088E-4	0.0	0.0	7.005462153291073	0.0
27	1.25278745208088E-4	0.0	0.0	7.177762187533929	0.0
28	1.25278745208088E-4	0.0	0.0	7.339705845506251	0.0
29	1.25278745208088E-4	0.0	0.0	7.515764242105351	0.0
30	1.25278745208088E-4	0.0	0.0	7.758220373664737	0.0
31	1.25278745208088E-4	0.0	0.0	7.943048282428403	0.0
32	1.6703832694411734E-4	0.0	0.0	8.138357846207812	0.0
33	1.6703832694411734E-4	0.0	0.0	8.321765929192454	0.0
34	1.6703832694411734E-4	0.0	0.0	8.50722023168216	0.0
35	1.6703832694411734E-4	0.0	0.0	8.72027761769938	0.0
36	1.6703832694411734E-4	0.0	0.0	8.910617791252204	0.0
37	1.6703832694411734E-4	0.0	0.0	9.117118922936868	0.0
38	1.6703832694411734E-4	0.0	0.0	9.338027110320462	0.0
39	1.6703832694411734E-4	0.0	0.0	9.608879757460349	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8550	0.0	40.657894	1
TAATCCG	40	3.460318E-7	39.375004	1
CTAATCC	1350	0.0	38.833332	11
TACGGGA	735	0.0	37.959187	4
GGGCGAT	5140	0.0	37.68969	7
GACTAAT	1415	0.0	37.5265	9
TCGTTAG	150	0.0	37.500004	1
CATATGC	1375	0.0	36.81818	33
AGGGCGA	2860	0.0	36.81818	6
ACTAATC	1425	0.0	36.789474	10
TAGGGAC	1995	0.0	36.76692	5
CGTAAGG	325	0.0	36.692307	2
ATAGGGA	2430	0.0	36.48148	4
ACGACCA	605	0.0	36.44628	28
GGCGATA	1070	0.0	36.378506	8
CGTTAGG	435	0.0	36.206894	2
GCGATAA	255	0.0	36.17647	9
CACGACC	610	0.0	36.14754	27
GGTACCT	1525	0.0	36.147537	8
CCCTACA	1385	0.0	36.064983	27
>>END_MODULE