##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547001_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1060421
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.675286513563954	31.0	31.0	33.0	30.0	34.0
2	32.038810057514894	33.0	31.0	34.0	30.0	34.0
3	32.09003310949142	33.0	31.0	34.0	30.0	34.0
4	35.76333267636156	37.0	35.0	37.0	35.0	37.0
5	35.72460088964666	37.0	35.0	37.0	33.0	37.0
6	35.78219311009495	37.0	35.0	37.0	33.0	37.0
7	36.07837453237912	37.0	35.0	37.0	35.0	37.0
8	35.99512269183654	37.0	35.0	37.0	35.0	37.0
9	37.78427530197912	39.0	38.0	39.0	35.0	39.0
10	37.48227260682314	39.0	37.0	39.0	35.0	39.0
11	37.13879110277899	39.0	37.0	39.0	34.0	39.0
12	36.129618330832756	37.0	35.0	39.0	33.0	39.0
13	35.66832135538621	37.0	35.0	39.0	31.0	39.0
14	36.616188287482046	38.0	35.0	41.0	31.0	41.0
15	36.89164963726671	38.0	35.0	41.0	32.0	41.0
16	37.08809142783856	38.0	35.0	41.0	33.0	41.0
17	37.04269530686397	38.0	35.0	41.0	33.0	41.0
18	36.962384750962116	38.0	35.0	40.0	33.0	41.0
19	36.82503081323361	37.0	35.0	40.0	32.0	41.0
20	36.62625032887881	37.0	35.0	40.0	32.0	41.0
21	36.527219849474875	36.0	35.0	40.0	32.0	41.0
22	36.431820946586306	36.0	35.0	40.0	32.0	41.0
23	36.384549155476925	36.0	35.0	40.0	32.0	41.0
24	36.271314883428374	36.0	35.0	40.0	32.0	41.0
25	36.152281971028486	35.0	35.0	40.0	32.0	41.0
26	35.97489770572254	35.0	35.0	40.0	31.0	41.0
27	35.912399886460186	35.0	35.0	40.0	31.0	41.0
28	35.93731734848706	36.0	35.0	40.0	31.0	41.0
29	35.9570236726734	36.0	35.0	40.0	31.0	41.0
30	35.864217136401486	36.0	35.0	40.0	31.0	41.0
31	35.61460495407013	36.0	35.0	40.0	31.0	41.0
32	35.31442606285617	35.0	34.0	40.0	30.0	41.0
33	35.08586495363634	35.0	34.0	40.0	29.0	41.0
34	34.864633951986995	35.0	34.0	40.0	28.0	41.0
35	34.67147576292812	35.0	34.0	40.0	27.0	41.0
36	34.46324714429458	35.0	34.0	40.0	25.0	41.0
37	34.31821418097152	35.0	34.0	40.0	24.0	41.0
38	34.16154998816508	35.0	34.0	40.0	23.0	41.0
39	34.02009202005618	35.0	34.0	40.0	23.0	41.0
40	33.72644261100073	35.0	33.0	40.0	22.0	41.0
41	33.61186830513541	35.0	34.0	40.0	21.0	41.0
42	33.46182601061277	35.0	33.0	40.0	20.0	41.0
43	33.27465318019919	35.0	33.0	40.0	18.0	41.0
44	33.04358740537956	35.0	33.0	39.0	18.0	41.0
45	32.91698202883572	35.0	33.0	39.0	18.0	41.0
46	32.85598738614192	35.0	33.0	39.0	18.0	41.0
47	32.78851607050407	35.0	33.0	39.0	18.0	41.0
48	32.69250608956254	35.0	33.0	39.0	17.0	41.0
49	32.69131411015059	35.0	33.0	39.0	17.0	41.0
50	32.519665302742965	35.0	33.0	38.0	15.0	41.0
51	32.14014905400779	35.0	32.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	6.0
14	14.0
15	32.0
16	112.0
17	286.0
18	593.0
19	1166.0
20	2008.0
21	3290.0
22	5172.0
23	7936.0
24	12304.0
25	16731.0
26	20465.0
27	21660.0
28	22644.0
29	25379.0
30	27806.0
31	32074.0
32	38455.0
33	51014.0
34	100287.0
35	215183.0
36	65545.0
37	76944.0
38	111376.0
39	201707.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.106890565162328	26.42705114289513	28.380803473337473	14.085254818605064
2	29.930093802367175	29.592492038539408	27.42505099389771	13.05236316519571
3	28.88692321257312	26.91336742671071	30.483270323767638	13.716439036948533
4	22.30350021359441	32.170619027725785	30.523160141113763	15.002720617566043
5	23.663714694446828	32.70059721563417	29.226788228448893	14.408899861470115
6	21.13933994140063	40.92827282749021	26.060875821961275	11.871511409147876
7	81.91680474075862	6.487046182601061	9.503678256088856	2.09247082055146
8	81.06204988396118	5.749697525794001	7.855653556464838	5.33259903377998
9	76.8153403223814	7.216662061577431	11.926583875649388	4.041413740391788
10	53.56014262259989	18.969824249048255	13.726529368995898	13.743503759355955
11	46.47852126655357	20.566077058074107	19.821750040785687	13.133651634586641
12	40.72552316485622	20.226777855210337	23.335826053991767	15.711872925941679
13	19.862865786324488	43.35966564223078	24.5475146191937	12.229953952251039
14	13.363654623965388	45.27003897508631	27.705128434838617	13.661177966109687
15	10.734793068036186	24.937736993137634	50.64054748067042	13.68692245815577
16	12.505504889095933	19.047340631692506	49.53042235112281	18.91673212808875
17	13.797067391158793	21.28456528114777	27.506999578469305	37.41136774922413
18	18.830917154601803	23.81195770359131	34.98384132339891	22.37328381840797
19	25.66867310247534	28.033205679631013	25.243181717449957	21.05493950044369
20	28.713784430900557	25.4414991781566	24.132679379227685	21.712037011715157
21	19.751683529466128	32.30613124410022	27.357530641132154	20.5846545853015
22	20.654155283609057	27.216077388131694	24.636441564246653	27.493325764012592
23	17.652800161445313	33.11448943391351	22.20174817360275	27.030962231038423
24	19.378624150219583	25.84313211450924	37.808096972806084	16.970146762465095
25	16.513158453104946	26.053143044130582	34.69914307619332	22.734555426571145
26	14.566478785312626	36.161581107880735	27.67730929508186	21.594630811724777
27	17.044928382218004	34.2514906815312	27.062836364047865	21.640744572202927
28	13.552636169974	31.90091482533824	36.55944195748669	17.987007047201065
29	13.861004261515003	26.323130153024128	35.97788048331748	23.837985102143396
30	17.603385825063818	32.142139772788354	30.873964208554906	19.380510193592922
31	27.504453419915297	28.13212865456267	24.43755829052801	19.925859634994026
32	28.721988719574583	27.66325827195048	25.977229798353672	17.637523210121266
33	28.681815995722452	27.020023179473057	23.797906680459928	20.500254144344556
34	19.60296900947831	27.494834598711265	27.964176492166793	24.93801989964363
35	19.877294018130534	26.10840411496943	30.350115661609873	23.664186205290164
36	29.16039950170734	23.56111393493716	27.580178061354875	19.698308502000618
37	22.300011033353734	28.973209696903403	27.99058109939354	20.736198170349322
38	21.934873036275214	31.213452015756005	24.179358952717838	22.672315995250944
39	21.433939916316255	29.501113237101112	25.519864280318856	23.545082566263776
40	25.884436464385374	24.6097540505139	24.600323833647202	24.905485651453528
41	17.605649077111828	21.79294827243142	28.33214355430532	32.26925909615144
42	22.934476024145127	24.94490395795632	24.37852513294248	27.74209488495607
43	22.23475393263619	23.226529840506743	27.67240558231118	26.866310644545894
44	21.083230151043782	27.232297361142415	27.31679210426802	24.367680383545782
45	18.882311836525304	31.501639443202272	23.22162612773606	26.39442259253636
46	24.249425464037397	27.733984898450707	26.79680994623833	21.219779691273562
47	23.115819094491716	23.93615365972571	27.893449865666557	25.05457738011601
48	23.795266219737254	19.977631525592194	32.10668215736957	24.120420097300975
49	21.917238530734494	18.77150678834161	33.72632190422483	25.584932776699066
50	20.099658531847258	27.627140541351032	28.866365339803718	23.406835586997996
51	18.09743488671009	29.56278685540931	25.44046185430126	26.89931640357933
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1451.0
1	1640.5
2	1830.0
3	5312.0
4	8794.0
5	6651.0
6	4508.0
7	5433.0
8	6358.0
9	7466.0
10	8574.0
11	9189.0
12	9804.0
13	10087.5
14	10371.0
15	10305.0
16	10239.0
17	10013.5
18	9788.0
19	9508.5
20	9229.0
21	8773.5
22	8318.0
23	8042.5
24	7767.0
25	7031.5
26	6357.0
27	6418.0
28	6139.5
29	5861.0
30	7073.5
31	8286.0
32	9326.5
33	10367.0
34	11659.0
35	12951.0
36	14899.5
37	16848.0
38	20895.5
39	24943.0
40	40493.0
41	56043.0
42	73600.5
43	91158.0
44	96717.0
45	102276.0
46	106722.5
47	111169.0
48	110757.5
49	110346.0
50	103795.5
51	97245.0
52	83318.5
53	69392.0
54	58610.0
55	47828.0
56	42442.0
57	37056.0
58	35486.0
59	33916.0
60	32283.0
61	30650.0
62	28406.0
63	26162.0
64	22359.0
65	18556.0
66	16066.5
67	13577.0
68	10601.5
69	7626.0
70	7073.0
71	6520.0
72	5519.0
73	4518.0
74	3789.0
75	2695.5
76	2331.0
77	1669.0
78	1007.0
79	777.0
80	547.0
81	361.0
82	175.0
83	194.0
84	213.0
85	114.0
86	15.0
87	14.5
88	14.0
89	12.5
90	11.0
91	8.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1060421.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.061837711009368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.34484598728051	20.386513287602757
2	7.66159354754517	3.8402722819258717
3	2.6070113130816397	1.9600948331765247
4	1.3243482653069216	1.3276240519191538
5	0.7844734982765328	0.9830173751197124
6	0.5196004791156346	0.7813285729295248
7	0.36180433299335946	0.6347237033633695
8	0.3113642291165759	0.624268782330653
9	0.25641960290451166	0.5783711826522903
>10	2.7058840817286858	16.469433710654453
>50	1.3805791456519076	25.539900078940082
>100	0.7348672169868102	23.80458302177209
>500	0.002276305266866733	0.411413995475383
>1k	0.004552610533733466	2.17620603846558
>5k	3.793842111444555E-4	0.48224908367255426
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5072	0.47830059947888626	No Hit
GAGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	3364	0.31723249539569665	TruSeq Adapter, Index 14 (95% over 23bp)
AAGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	2932	0.27649395853156433	Illumina PCR Primer Index 3 (95% over 21bp)
AAGAGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	2663	0.25112667516014864	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2508	0.23650983901676784	No Hit
TAGAGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	2149	0.20265536046532462	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT	2049	0.19322514359862733	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1601	0.1509777720358235	TruSeq Adapter, Index 14 (96% over 25bp)
AGAGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	1195	0.11269109155703254	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	1191	0.11231388288236466	Illumina PCR Primer Index 3 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1158	0.10920191131635454	TruSeq Adapter, Index 14 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.430216866697284E-5	0.0	0.0	0.12825094938708306	0.0
2	9.430216866697284E-5	0.0	0.0	0.558740349351814	0.0
3	9.430216866697284E-5	0.0	0.0	0.8885150331802181	0.0
4	9.430216866697284E-5	0.0	0.0	2.009484912124524	0.0
5	9.430216866697284E-5	0.0	0.0	3.1052761120347485	0.0
6	9.430216866697284E-5	0.0	0.0	5.06562959428378	0.0
7	9.430216866697284E-5	0.0	0.0	5.750829151818004	0.0
8	9.430216866697284E-5	0.0	0.0	7.473069658182929	0.0
9	9.430216866697284E-5	0.0	0.0	8.19391543547327	0.0
10	9.430216866697284E-5	0.0	0.0	9.391552977543824	0.0
11	9.430216866697284E-5	0.0	0.0	10.678211766836002	0.0
12	9.430216866697284E-5	0.0	0.0	11.686867762897943	0.0
13	9.430216866697284E-5	0.0	0.0	12.136877711776737	0.0
14	9.430216866697284E-5	0.0	0.0	12.318597990797995	0.0
15	9.430216866697284E-5	0.0	0.0	12.534361352708029	0.0
16	9.430216866697284E-5	0.0	0.0	12.98446560375549	0.0
17	9.430216866697284E-5	0.0	0.0	13.417218255768228	0.0
18	9.430216866697284E-5	0.0	0.0	14.053474987764293	0.0
19	9.430216866697284E-5	0.0	0.0	14.313654671116472	0.0
20	9.430216866697284E-5	0.0	0.0	14.594580831575383	0.0
21	9.430216866697284E-5	0.0	0.0	14.914830996368423	0.0
22	9.430216866697284E-5	0.0	0.0	15.201792495622021	0.0
23	9.430216866697284E-5	0.0	0.0	15.541940418003794	0.0
24	9.430216866697284E-5	0.0	0.0	15.77382945075588	0.0
25	9.430216866697284E-5	0.0	0.0	15.946968232428441	0.0
26	9.430216866697284E-5	0.0	0.0	16.13198908735304	0.0
27	9.430216866697284E-5	0.0	0.0	16.293811608785568	0.0
28	9.430216866697284E-5	0.0	0.0	16.45261646082075	0.0
29	9.430216866697284E-5	0.0	0.0	16.620002810204625	0.0
30	9.430216866697284E-5	0.0	0.0	16.859247412112737	0.0
31	9.430216866697284E-5	0.0	0.0	17.076142400046773	0.0
32	9.430216866697284E-5	0.0	0.0	17.241831310394645	0.0
33	9.430216866697284E-5	0.0	0.0	17.41827066797055	0.0
34	9.430216866697284E-5	0.0	0.0	17.59216386699245	0.0
35	9.430216866697284E-5	0.0	0.0	17.826599058298545	0.0
36	9.430216866697284E-5	0.0	0.0	18.00586748093446	0.0
37	9.430216866697284E-5	0.0	0.0	18.178534751763685	0.0
38	9.430216866697284E-5	0.0	0.0	18.352993763797585	0.0
39	9.430216866697284E-5	0.0	0.0	18.545275885709543	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGTA	20	7.0330384E-4	45.000004	33
CGGAATT	20	7.0330384E-4	45.000004	39
AGTCAAC	20	7.0330384E-4	45.000004	26
TAGACAA	20	7.0330384E-4	45.000004	34
GGGTATA	40	6.8139343E-9	45.000004	7
ATTACCG	20	7.0330384E-4	45.000004	16
CATCTCG	20	7.0330384E-4	45.000004	1
ATTCGGT	20	7.0330384E-4	45.000004	38
AAGCGTG	20	7.0330384E-4	45.000004	1
ATATGCG	40	6.8139343E-9	45.000004	1
GACCCAA	20	7.0330384E-4	45.000004	24
GTAATCG	20	7.0330384E-4	45.000004	35
TACGCTT	20	7.0330384E-4	45.000004	10
CACTCGA	20	7.0330384E-4	45.000004	38
GTTGTGC	20	7.0330384E-4	45.000004	22
TACGATT	20	7.0330384E-4	45.000004	17
TAATTCG	20	7.0330384E-4	45.000004	34
TACGAGT	20	7.0330384E-4	45.000004	14
TACGACC	35	1.2118107E-7	45.000004	32
CGCTAGC	20	7.0330384E-4	45.000004	29
>>END_MODULE