FastQCFastQC Report
Thu 26 May 2016
SRR1547000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547000_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3937585
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT897242.2786555718797183No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT68430.1737867246040403No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT59470.15103166026892118TruSeq Adapter, Index 23 (96% over 26bp)
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC55410.14072077174207032TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC46860.1190069547705002Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT44630.11334358496388015No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG42450.10780719654305876TruSeq Adapter, Index 23 (95% over 24bp)
GAGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT40170.10201684535064005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT432900.044.142411
CGTTCGG4550.042.5274745
TCGTTAG1800.042.51
TCGACGG1350.041.6666642
GCGCGAC5100.041.029419
ACGCCGA1050.040.71428712
CGTTAGG5050.040.0990072
AGGGCGA45750.039.885256
GTTTTTT492900.038.8877072
CGCGACC5400.038.7510
CGTTCGA1350.038.33333214
TACGGGA12050.038.2780084
TATTACG1000.038.251
TAGGGCG15850.038.186125
GGCGCGA5550.038.108118
AGGGATT67300.038.0794946
CGACGGT1300.038.07692728
TACGAAT2550.037.94117712
GACCGAT24950.037.515039
TAATACG900.037.51