##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547000_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3937585
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56130724797052	31.0	31.0	33.0	30.0	34.0
2	31.916705290171514	31.0	31.0	34.0	30.0	34.0
3	31.817222993281415	31.0	31.0	34.0	30.0	34.0
4	35.626627488676434	37.0	35.0	37.0	33.0	37.0
5	35.60983166077685	37.0	35.0	37.0	33.0	37.0
6	35.686281819948015	37.0	35.0	37.0	33.0	37.0
7	36.11393861973773	37.0	35.0	37.0	35.0	37.0
8	36.06162355860254	37.0	35.0	37.0	35.0	37.0
9	37.825299263380984	39.0	38.0	39.0	35.0	39.0
10	37.313880462263036	39.0	37.0	39.0	34.0	39.0
11	37.047615226084005	39.0	37.0	39.0	33.0	39.0
12	36.772327708481214	39.0	35.0	39.0	33.0	39.0
13	36.689610256032566	39.0	35.0	39.0	33.0	39.0
14	37.837581664903745	40.0	36.0	41.0	33.0	41.0
15	37.91846449029037	40.0	36.0	41.0	33.0	41.0
16	37.97710195462447	40.0	36.0	41.0	33.0	41.0
17	37.94168633819968	40.0	36.0	41.0	33.0	41.0
18	37.73509549635119	39.0	36.0	41.0	33.0	41.0
19	37.566510183272236	39.0	36.0	41.0	33.0	41.0
20	37.29247800364944	39.0	35.0	41.0	33.0	41.0
21	37.23747779413016	39.0	35.0	41.0	33.0	41.0
22	37.16505980188364	39.0	35.0	41.0	33.0	41.0
23	37.075811696763374	38.0	35.0	41.0	32.0	41.0
24	36.98377431852265	38.0	35.0	41.0	32.0	41.0
25	36.8858500832363	38.0	35.0	41.0	32.0	41.0
26	36.75300368119037	38.0	35.0	41.0	32.0	41.0
27	36.656119677416484	38.0	35.0	41.0	32.0	41.0
28	36.63194699289031	38.0	35.0	41.0	31.0	41.0
29	36.583720986340616	38.0	35.0	41.0	31.0	41.0
30	36.46533039921678	38.0	35.0	40.0	31.0	41.0
31	36.248357051339845	38.0	35.0	40.0	31.0	41.0
32	35.94350979089975	38.0	35.0	41.0	30.0	41.0
33	35.541513643514996	38.0	35.0	41.0	27.0	41.0
34	35.17720201595648	38.0	35.0	41.0	24.0	41.0
35	34.887339320928945	38.0	34.0	41.0	23.0	41.0
36	34.74434609030662	38.0	34.0	41.0	22.0	41.0
37	34.65229829959226	38.0	34.0	41.0	21.0	41.0
38	34.57280795208231	38.0	34.0	40.0	21.0	41.0
39	34.47752061225345	38.0	34.0	40.0	20.0	41.0
40	34.38652219571133	38.0	34.0	40.0	19.0	41.0
41	34.33086244487421	38.0	34.0	40.0	18.0	41.0
42	34.222579322097175	38.0	34.0	40.0	18.0	41.0
43	34.094093206876806	38.0	34.0	40.0	18.0	41.0
44	33.880717495622314	37.0	33.0	40.0	17.0	41.0
45	33.811547179298984	37.0	33.0	40.0	17.0	41.0
46	33.78239276104516	37.0	33.0	40.0	18.0	41.0
47	33.74807451775644	37.0	33.0	40.0	18.0	41.0
48	33.63530006336371	37.0	33.0	40.0	17.0	41.0
49	33.56941856493257	37.0	33.0	40.0	15.0	41.0
50	33.451698693488524	37.0	33.0	40.0	15.0	41.0
51	33.072601099404835	36.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	1.0
11	4.0
12	11.0
13	10.0
14	35.0
15	99.0
16	267.0
17	727.0
18	1778.0
19	3607.0
20	6065.0
21	10003.0
22	16025.0
23	24896.0
24	40783.0
25	67307.0
26	94387.0
27	99018.0
28	90053.0
29	86529.0
30	91510.0
31	104082.0
32	125392.0
33	157561.0
34	247626.0
35	415491.0
36	302548.0
37	349359.0
38	512826.0
39	1088821.0
40	761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.711866537484273	23.93901338002862	26.673328956708236	19.675791125778872
2	36.97680177062844	24.921138210349746	26.175714302040465	11.926345716981347
3	25.613872462435733	24.297735794909826	37.70491303679794	12.383478705856508
4	22.153121773879167	27.31821662262529	37.05494611544894	13.473715488046606
5	20.525702937206436	31.203262913689482	35.43562869119016	12.835405457913923
6	18.94666400852299	38.957635200256	31.91098096929971	10.184719821921306
7	80.69479135053592	2.8508590925656208	14.495331529351112	1.9590180275473417
8	80.12825119965665	2.5057236859648744	14.030097128061996	3.3359279863164857
9	74.31423067692508	5.050786205250173	17.01578505606863	3.6191980617561272
10	35.27934000154917	30.784478303325514	21.811414864694985	12.124766830430326
11	25.67571239731968	24.255476389716033	34.71163162192054	15.35717959104375
12	24.597081713791574	21.62871404680788	35.58760001371399	18.186604225686555
13	19.64186169949347	27.733826698344288	37.29882656501384	15.325485037148404
14	16.832728690301288	30.690029548568475	35.42168613502947	17.05555562610077
15	15.039091219618117	26.15143546107576	43.21791148635521	15.591561832950907
16	17.30009637887182	23.97129204829864	42.39789109314466	16.33072047968488
17	17.222205996822925	25.08684891881699	35.030913618372686	22.6600314659874
18	18.07308286678256	25.737019010383268	37.84433859840486	18.345559524429316
19	20.7446950351548	28.76362034089423	33.01165562140246	17.480029002548516
20	22.098468985431424	26.998299719244155	32.22955186999138	18.673679425333038
21	19.40318748674632	29.549076400890396	34.0845213500153	16.963214762347985
22	19.319836905108083	25.840965972798045	33.163855510420724	21.675341611673147
23	17.193000278089237	29.98289560733292	32.27638260507392	20.547721509503923
24	17.360387140849024	26.968230527087034	37.86160806687347	17.809774265190466
25	17.841468819085808	27.978367451115343	35.0392943898354	19.140869339963455
26	16.699982349587376	31.338371108179253	33.359736996153735	18.60190954607964
27	17.858687495000108	29.578053553129646	33.23587935244572	19.32737959942452
28	16.791383551085246	29.014535559232375	36.63037115389255	17.563709735789832
29	16.83435405203951	26.975773221403475	35.680956728553156	20.50891599800385
30	18.28534495128359	29.70079376064263	33.70987546935495	18.303985818718836
31	20.621446902098622	29.466767066615706	31.41999474297063	18.491791288315046
32	21.87048660536852	29.116323838088576	31.294486341247236	17.71870321529567
33	20.16931697982393	29.441396185733133	31.028434941721894	19.360851892721044
34	18.09075867568573	29.181211326231686	32.05622227837621	20.67180771970637
35	17.048393875941727	28.546812322781605	34.0808642861043	20.32392951517237
36	20.933110015402843	28.515016183777618	30.591949126177592	19.959924674641943
37	18.041616879381653	29.43413285046545	33.198267465972165	19.325982804180736
38	17.750296184082377	29.96781021870004	32.3332194733574	19.948674123860183
39	17.78186375659192	29.239648159976227	31.03285389394769	21.945634189484164
40	19.956927913937093	27.340209798645613	31.462304940718738	21.240557346698548
41	17.01499777147668	27.220847295994883	31.497783539910884	24.266371392617554
42	19.18198591268506	29.187534999244463	29.411200012190214	22.219279075880266
43	18.949863939445116	28.59067169343646	31.113055337218114	21.346409029900308
44	19.532022800777636	30.244375676969515	29.691955856191043	20.531645666061813
45	17.90323256513828	30.454936210900847	29.621633564735745	22.020197659225136
46	20.18897369834556	29.82536250011111	29.315151291972107	20.670512509571225
47	19.318109958261218	28.444084381670493	30.662779343176084	21.575026316892206
48	19.225743698231277	26.878835631484783	31.928732967034364	21.96668770324958
49	19.78811885965636	26.028238120573903	31.252861842982437	22.930781176787296
50	18.108383692034586	28.34003583414707	31.845737933276364	21.70584254054198
51	17.32467997516244	28.637299258301724	30.419559196817335	23.618461569718498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2929.0
1	3584.5
2	4240.0
3	62377.0
4	120514.0
5	78801.0
6	37088.0
7	37786.5
8	38485.0
9	41749.5
10	45014.0
11	48168.0
12	51322.0
13	52194.0
14	53066.0
15	51744.5
16	50423.0
17	48520.0
18	46617.0
19	44249.0
20	41881.0
21	40491.5
22	39102.0
23	38146.5
24	37191.0
25	38641.0
26	42944.5
27	45798.0
28	50019.5
29	54241.0
30	63340.5
31	72440.0
32	81229.5
33	90019.0
34	99712.5
35	109406.0
36	119782.5
37	130159.0
38	145235.5
39	160312.0
40	192385.0
41	224458.0
42	262218.0
43	299978.0
44	344571.0
45	389164.0
46	401835.0
47	414506.0
48	380290.0
49	346074.0
50	302602.5
51	259131.0
52	224789.0
53	190447.0
54	171045.5
55	151644.0
56	134325.0
57	117006.0
58	102138.0
59	87270.0
60	77294.0
61	67318.0
62	56995.5
63	46673.0
64	39431.0
65	32189.0
66	25344.5
67	18500.0
68	13550.5
69	8601.0
70	7213.0
71	5825.0
72	4547.0
73	3269.0
74	2709.0
75	1650.5
76	1152.0
77	984.0
78	816.0
79	651.0
80	486.0
81	385.0
82	284.0
83	220.5
84	157.0
85	112.0
86	67.0
87	48.0
88	29.0
89	23.5
90	18.0
91	16.5
92	15.0
93	15.5
94	16.0
95	10.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3937585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.688141096675494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35686454547786	21.905365037804078
2	9.173205521936701	5.263244286442458
3	3.584584683147876	3.0850521348938424
4	1.9051070106018957	2.1861591489765138
5	1.2395894798758642	1.7780758950316695
6	0.8628301342913557	1.4851795581008327
7	0.6689200999309326	1.3433051946454606
8	0.5162309281121602	1.1847764563319518
9	0.4158059673454631	1.073583023404166
>10	4.147437266444416	25.761692201894714
>50	0.7701027958650071	15.596310628527144
>100	0.3541818681217442	14.575707653376208
>500	0.002524764346891965	0.4635829613798062
>1k	0.0022542538809891856	1.502783804296718
>5k	2.7051046571870233E-4	0.4741888993108262
>10k+	9.017015523956743E-5	2.320993115583687
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89724	2.2786555718797183	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	6843	0.1737867246040403	No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	5947	0.15103166026892118	TruSeq Adapter, Index 23 (96% over 26bp)
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	5541	0.14072077174207032	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	4686	0.1190069547705002	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT	4463	0.11334358496388015	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	4245	0.10780719654305876	TruSeq Adapter, Index 23 (95% over 24bp)
GAGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	4017	0.10201684535064005	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16532976430984983	0.0
2	0.0	0.0	0.0	0.463913794876809	0.0
3	0.0	0.0	0.0	0.7515774262650838	0.0
4	0.0	0.0	0.0	1.1578924645436226	0.0
5	0.0	0.0	0.0	1.9364661334295006	0.0
6	0.0	0.0	0.0	3.467226739232296	0.0
7	0.0	0.0	0.0	4.343195131025743	0.0
8	0.0	0.0	0.0	6.198621744038541	0.0
9	0.0	0.0	0.0	7.242713490629408	0.0
10	0.0	0.0	0.0	8.548767836122902	0.0
11	0.0	0.0	0.0	9.580034462748106	0.0
12	0.0	0.0	0.0	10.419584593094498	0.0
13	0.0	0.0	0.0	10.928932327810067	0.0
14	0.0	0.0	0.0	11.212761121347222	0.0
15	0.0	0.0	0.0	11.460349427377441	0.0
16	0.0	0.0	0.0	11.847617257786181	0.0
17	0.0	0.0	0.0	12.288293459061837	0.0
18	0.0	0.0	0.0	12.85658595306514	0.0
19	0.0	0.0	0.0	13.192604096165542	0.0
20	0.0	0.0	0.0	13.524279475871632	0.0
21	0.0	0.0	0.0	13.904131593349732	2.5396277159731156E-5
22	0.0	0.0	0.0	14.342979262669886	2.5396277159731156E-5
23	0.0	0.0	0.0	14.814257977923017	2.5396277159731156E-5
24	0.0	0.0	0.0	15.187659441002543	2.5396277159731156E-5
25	0.0	0.0	0.0	15.518852291442597	2.5396277159731156E-5
26	0.0	0.0	0.0	15.832293144148	2.5396277159731156E-5
27	0.0	0.0	0.0	16.153276691169843	2.5396277159731156E-5
28	0.0	0.0	0.0	16.482260065496998	2.5396277159731156E-5
29	0.0	0.0	0.0	16.829198607776085	2.5396277159731156E-5
30	0.0	0.0	0.0	17.301467777838447	2.5396277159731156E-5
31	0.0	0.0	0.0	17.669205871111355	2.5396277159731156E-5
32	0.0	0.0	0.0	18.003166915761817	2.5396277159731156E-5
33	0.0	0.0	0.0	18.34502620260896	2.5396277159731156E-5
34	0.0	0.0	0.0	18.69701860404283	2.5396277159731156E-5
35	0.0	0.0	0.0	19.08459118977749	2.5396277159731156E-5
36	0.0	0.0	0.0	19.446691309520936	2.5396277159731156E-5
37	0.0	0.0	0.0	19.811915171355032	2.5396277159731156E-5
38	0.0	0.0	0.0	20.193951368668866	2.5396277159731156E-5
39	0.0	0.0	0.0	20.583885808179378	2.5396277159731156E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	43290	0.0	44.14241	1
CGTTCGG	455	0.0	42.52747	45
TCGTTAG	180	0.0	42.5	1
TCGACGG	135	0.0	41.666664	2
GCGCGAC	510	0.0	41.02941	9
ACGCCGA	105	0.0	40.714287	12
CGTTAGG	505	0.0	40.099007	2
AGGGCGA	4575	0.0	39.88525	6
GTTTTTT	49290	0.0	38.887707	2
CGCGACC	540	0.0	38.75	10
CGTTCGA	135	0.0	38.333332	14
TACGGGA	1205	0.0	38.278008	4
TATTACG	100	0.0	38.25	1
TAGGGCG	1585	0.0	38.18612	5
GGCGCGA	555	0.0	38.10811	8
AGGGATT	6730	0.0	38.079494	6
CGACGGT	130	0.0	38.076927	28
TACGAAT	255	0.0	37.941177	12
GACCGAT	2495	0.0	37.51503	9
TAATACG	90	0.0	37.5	1
>>END_MODULE