##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546999_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4613047
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59809687609946	31.0	31.0	33.0	30.0	34.0
2	31.974274920676073	31.0	31.0	34.0	30.0	34.0
3	32.08080234170603	33.0	31.0	34.0	30.0	34.0
4	35.75566886702	37.0	35.0	37.0	35.0	37.0
5	35.69802453779465	37.0	35.0	37.0	33.0	37.0
6	35.747614754412865	37.0	35.0	37.0	33.0	37.0
7	36.093478345223886	37.0	35.0	37.0	35.0	37.0
8	36.01933212473231	37.0	35.0	37.0	35.0	37.0
9	37.785819437781576	39.0	38.0	39.0	35.0	39.0
10	37.30928906642399	39.0	37.0	39.0	34.0	39.0
11	37.05278311710243	39.0	37.0	39.0	33.0	39.0
12	36.59289814302781	39.0	35.0	39.0	33.0	39.0
13	36.4450661352464	39.0	35.0	39.0	32.0	39.0
14	37.46085548228752	40.0	35.0	41.0	32.0	41.0
15	37.581354362962266	40.0	35.0	41.0	32.0	41.0
16	37.677167173887455	40.0	35.0	41.0	33.0	41.0
17	37.613350351730645	40.0	35.0	41.0	33.0	41.0
18	37.536074095928356	39.0	36.0	41.0	33.0	41.0
19	37.49636346648972	39.0	35.0	41.0	33.0	41.0
20	37.34915859300805	39.0	35.0	41.0	32.0	41.0
21	37.28338753106136	39.0	35.0	41.0	32.0	41.0
22	37.20628924873299	39.0	35.0	41.0	32.0	41.0
23	37.11670160741913	39.0	35.0	41.0	32.0	41.0
24	37.00874606306851	39.0	35.0	41.0	32.0	41.0
25	36.885845732766214	39.0	35.0	41.0	31.0	41.0
26	36.73286094852274	38.0	35.0	41.0	31.0	41.0
27	36.64330560690147	38.0	35.0	41.0	31.0	41.0
28	36.67006969579976	38.0	35.0	41.0	31.0	41.0
29	36.67063916756105	38.0	35.0	41.0	31.0	41.0
30	36.57168483217275	38.0	35.0	41.0	31.0	41.0
31	36.47142203406989	38.0	35.0	41.0	31.0	41.0
32	36.38615973346901	38.0	35.0	41.0	30.0	41.0
33	36.27231068749137	38.0	35.0	41.0	30.0	41.0
34	36.08975911149399	38.0	35.0	41.0	30.0	41.0
35	36.009280633819685	38.0	35.0	41.0	30.0	41.0
36	35.90511629298379	38.0	35.0	41.0	29.0	41.0
37	35.82537897402736	38.0	35.0	41.0	29.0	41.0
38	35.718453768192695	38.0	35.0	41.0	29.0	41.0
39	35.615165421033	38.0	35.0	40.0	29.0	41.0
40	35.463303972406955	38.0	35.0	40.0	28.0	41.0
41	35.3913194467778	38.0	35.0	40.0	28.0	41.0
42	35.30549417770944	37.0	34.0	40.0	27.0	41.0
43	35.188743578810275	37.0	34.0	40.0	27.0	41.0
44	34.97169398013938	37.0	34.0	40.0	26.0	41.0
45	34.88733195217824	37.0	34.0	40.0	26.0	41.0
46	34.85061825730369	37.0	34.0	40.0	26.0	41.0
47	34.78896117902116	36.0	34.0	40.0	26.0	41.0
48	34.702966390760814	36.0	34.0	40.0	26.0	41.0
49	34.61865096973866	36.0	34.0	40.0	26.0	41.0
50	34.50857188318263	36.0	34.0	40.0	26.0	41.0
51	34.14955516386458	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	10.0
12	9.0
13	24.0
14	46.0
15	141.0
16	363.0
17	914.0
18	1939.0
19	3744.0
20	6200.0
21	10116.0
22	15054.0
23	22192.0
24	32571.0
25	45795.0
26	59394.0
27	70698.0
28	79463.0
29	89548.0
30	103326.0
31	124246.0
32	155077.0
33	201144.0
34	345830.0
35	519296.0
36	358069.0
37	462490.0
38	668109.0
39	1236365.0
40	872.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.93906587121267	24.302830645341356	26.8940897415526	12.864013741893373
2	32.5747168845234	25.600194405129624	27.82217480116721	14.002913909179767
3	30.85392366477081	25.158989275418175	29.803294872131154	14.183792187679856
4	26.135111998642113	28.82478760784358	29.29213597867093	15.747964414843377
5	23.806629327643964	32.437258931027586	28.574540862037605	15.181570879290845
6	22.447982862520153	41.08202235962477	25.94031667138878	10.52967810646629
7	87.17836605610132	3.7400876253807946	7.146252791267897	1.9352935272499934
8	86.5844635877328	3.3298598518506313	6.897133283055647	3.1885432773609286
9	81.20626128456962	5.066499430853404	9.908700258202442	3.8185390263745416
10	45.573327130636216	24.945659560806554	15.447945793745435	14.033067514811792
11	35.14624932284453	24.82643250762457	23.368350680146982	16.65896748938391
12	31.23562365612143	21.557898716401546	27.479212763277722	19.727264864199302
13	25.147174958330144	26.535671542041516	29.752070594554965	18.56508290507337
14	21.313028026811782	28.178945499579775	31.113903673645638	19.3941227999628
15	21.06434207152019	22.92541133875289	37.22663133499399	18.783615254732936
16	24.32266569146163	21.474764943864653	34.18577786005649	20.016791504617228
17	23.936977013240924	22.04332624402049	28.561599307355856	25.458097435382733
18	25.252441607466825	21.931512945781822	31.18504970792624	21.63099573882512
19	27.400804717576037	25.36598911738814	25.9178586300985	21.31534753493732
20	28.750303216073885	24.61212729894146	25.686904989261976	20.950664495722677
21	26.62203528383734	26.2099865880404	27.342253395640668	19.82572473248159
22	25.759676846995056	22.535235387803333	27.477044998674412	24.2280427665272
23	24.47785595941251	26.0951384193571	26.173394721536546	23.253610899693847
24	24.085295467399312	23.946471822203417	31.085137437359734	20.88309527303754
25	24.343693008113725	23.82620424201184	28.663809408401864	23.166293341472567
26	21.641205910106702	26.98006328571983	27.48014490205714	23.898585902116324
27	22.762048598247535	25.268287966717008	27.402950804533315	24.56671263050214
28	21.154867921354366	26.691685560541657	29.71153339647309	22.44191312163089
29	23.153872050295607	24.575600465375704	28.737188240223873	23.533339244104816
30	23.677408879640723	26.214733992521644	27.965529074384026	22.142328053453607
31	26.55149622364567	25.809708854039425	24.971434281939896	22.667360640375005
32	27.625233386956605	25.81998405825911	24.74479449266396	21.80998806212033
33	25.898630558067154	25.417213394964328	24.73005369336146	23.954102353607063
34	22.061665532564483	27.87593536332927	27.172669170723818	22.889729933382426
35	21.902768387141947	27.640060896843238	26.708203926818868	23.748966789195947
36	25.990218612556948	27.556732025492042	24.030884575856263	22.422164786094744
37	23.072927720008053	28.75444364646621	25.45163749686487	22.720991136660867
38	23.514089494427438	28.728452148872535	24.38906432126098	23.36839403543905
39	23.600409880931196	26.794871155659155	25.476458401572756	24.128260561836896
40	25.04288380326496	24.409072788549523	26.485357725598718	24.0626856825868
41	20.74747991945454	24.693180017459177	27.2449857978902	27.314354265196084
42	23.16167600286752	25.104946903857687	26.383017558676507	25.350359534598283
43	22.562614254743124	23.770232559954408	28.4399660354642	25.227187149838276
44	23.01210024523921	25.633144427099918	27.087432666521714	24.267322661139158
45	21.815992770071496	26.11202530561687	26.239988450150193	25.831993474161436
46	23.78196016645831	25.8502460521213	26.504867607028498	23.862926174391895
47	22.274214851918916	25.303102266246146	28.452257260764956	23.970425621069978
48	23.013184127540864	24.62454859011842	28.467843488262744	23.89442379407797
49	23.78143990295351	23.695813201122814	28.32242983867279	24.20031705725088
50	22.039749432425033	25.663970039758972	28.022541283451048	24.27373924436495
51	21.512462370316193	26.429884629400046	26.553230435328317	25.50442256495544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2490.0
1	4784.5
2	7079.0
3	25394.0
4	43709.0
5	30135.5
6	16562.0
7	16649.5
8	16737.0
9	17788.5
10	18840.0
11	19350.0
12	19860.0
13	20161.5
14	20463.0
15	19605.0
16	18747.0
17	18721.0
18	18695.0
19	18275.5
20	17856.0
21	18972.0
22	20088.0
23	20711.5
24	21335.0
25	21359.0
26	26430.5
27	31478.0
28	36764.5
29	42051.0
30	48215.0
31	54379.0
32	62757.0
33	71135.0
34	81427.0
35	91719.0
36	99255.0
37	106791.0
38	120650.0
39	134509.0
40	166914.5
41	199320.0
42	230182.5
43	261045.0
44	303939.0
45	346833.0
46	362974.0
47	379115.0
48	369529.5
49	359944.0
50	337244.0
51	314544.0
52	301752.5
53	288961.0
54	277891.5
55	266822.0
56	261924.5
57	257027.0
58	250177.0
59	243327.0
60	226231.5
61	209136.0
62	196811.0
63	184486.0
64	165888.0
65	147290.0
66	128057.5
67	108825.0
68	93936.5
69	79048.0
70	68438.0
71	57828.0
72	50683.0
73	43538.0
74	37766.0
75	25112.5
76	18231.0
77	14307.5
78	10384.0
79	7933.0
80	5482.0
81	3967.5
82	2453.0
83	1710.5
84	968.0
85	648.5
86	329.0
87	213.5
88	98.0
89	72.0
90	46.0
91	44.0
92	42.0
93	28.0
94	14.0
95	10.0
96	6.0
97	4.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4613047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.541219619092622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35706409500116	15.311925981877348
2	8.463270102529046	3.307652395384412
3	2.8441435991059962	1.6673410409510037
4	1.4489848696423167	1.1325972624969123
5	0.8532642407432335	0.8336911960740921
6	0.6192256750508888	0.7260254945970179
7	0.45391962664661073	0.6209100179602564
8	0.3571319846218532	0.5583035635598433
9	0.30916534385312305	0.5437321094561549
>10	3.4317235889954287	16.00988741717654
>50	1.5971301821939325	22.78255341813059
>100	1.2575087389765294	34.08652013002103
>500	0.004526031902952905	0.5822501704905798
>1k	0.0027156191417702998	1.1493984966619515
>5k	1.1315079757376247E-4	0.11059945136492856
>10k+	1.1315079757376247E-4	0.5766118537974023
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26078	0.5653096532508773	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	5002	0.1084315854575078	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.503293809926497E-5	0.0	0.0	0.08100936322565107	0.0
2	6.503293809926497E-5	0.0	0.0	0.2754361704964203	0.0
3	6.503293809926497E-5	0.0	0.0	0.4094040229809061	0.0
4	6.503293809926497E-5	0.0	0.0	0.7456459906001391	0.0
5	6.503293809926497E-5	0.0	0.0	1.2495645502853103	0.0
6	6.503293809926497E-5	0.0	0.0	2.132495073212998	0.0
7	6.503293809926497E-5	0.0	0.0	2.540663470370018	0.0
8	6.503293809926497E-5	0.0	0.0	3.6467653592083495	0.0
9	6.503293809926497E-5	0.0	0.0	4.15341530229369	0.0
10	6.503293809926497E-5	0.0	0.0	4.93029878082751	0.0
11	1.0838823016544163E-4	0.0	0.0	5.613816637896817	0.0
12	1.0838823016544163E-4	0.0	0.0	6.1491894619759995	0.0
13	1.0838823016544163E-4	0.0	0.0	6.409646379063556	0.0
14	1.0838823016544163E-4	0.0	0.0	6.539842321138284	0.0
15	1.0838823016544163E-4	0.0	0.0	6.683760213151958	0.0
16	1.3006587619852995E-4	0.0	0.0	6.947468777144477	0.0
17	1.3006587619852995E-4	0.0	0.0	7.236800318748108	0.0
18	1.3006587619852995E-4	0.0	0.0	7.616549321955748	0.0
19	1.3006587619852995E-4	0.0	0.0	7.822682058084386	0.0
20	1.3006587619852995E-4	0.0	0.0	8.040195558380393	0.0
21	1.3006587619852995E-4	0.0	0.0	8.311512976130528	0.0
22	1.3006587619852995E-4	0.0	0.0	8.58261361742033	0.0
23	1.3006587619852995E-4	0.0	0.0	8.883347600837364	0.0
24	1.3006587619852995E-4	0.0	0.0	9.129020363330355	0.0
25	1.3006587619852995E-4	0.0	0.0	9.339271852205277	0.0
26	1.3006587619852995E-4	0.0	0.0	9.542543139057546	0.0
27	1.3006587619852995E-4	0.0	0.0	9.748025545805191	0.0
28	1.3006587619852995E-4	0.0	0.0	9.963241215621693	0.0
29	1.3006587619852995E-4	0.0	0.0	10.19521370582177	0.0
30	1.3006587619852995E-4	0.0	0.0	10.557252072220379	0.0
31	1.5174352223161827E-4	0.0	0.0	10.82013688566364	0.0
32	1.734211682647066E-4	0.0	0.0	11.069191360937792	0.0
33	1.734211682647066E-4	0.0	0.0	11.312046029446481	0.0
34	1.734211682647066E-4	0.0	0.0	11.564092019873199	0.0
35	1.734211682647066E-4	0.0	0.0	11.867860873734866	0.0
36	1.734211682647066E-4	0.0	0.0	12.129965291920936	0.0
37	1.734211682647066E-4	0.0	0.0	12.388731352617912	0.0
38	2.1677646033088326E-4	0.0	0.0	12.704314523567612	0.0
39	2.1677646033088326E-4	0.0	0.0	13.167457431064543	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	75	0.0	45.000004	1
CAATACG	70	0.0	41.785717	1
CGTTTTT	14745	0.0	40.910477	1
AGGGTAC	1930	0.0	38.937824	6
TAGGGTA	1565	0.0	38.530354	5
GGCGATA	2080	0.0	38.293266	8
GGGCGAT	10560	0.0	38.18182	7
ACGGGTA	365	0.0	37.602737	5
ACGGGAT	1085	0.0	37.53456	5
AACACGT	2225	0.0	37.415733	41
AAGGGAT	4025	0.0	37.341614	5
CACGACC	2405	0.0	37.32848	27
CGTATGG	585	0.0	37.307693	2
GCGATAA	525	0.0	37.285717	9
AGGGCGA	5305	0.0	37.0688	6
CGCATGA	85	0.0	37.058823	11
CGTAAGG	735	0.0	37.040817	2
TAAGGGA	3720	0.0	36.83468	4
ACAACGA	1170	0.0	36.73077	13
TTGATCC	2665	0.0	36.726078	17
>>END_MODULE