FastQCFastQC Report
Thu 26 May 2016
SRR1546998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546998_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2105447
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96390.4578125215215581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAA2750.041.7272729
CGTAAGG3900.041.538462
CGTTTTT59800.041.0493351
GGCGATA11550.039.9350668
CATATGC12300.039.51219633
AGGGTAC10250.039.512196
GGGCGAT45850.039.405677
GCGTAAG2750.039.2727281
TAGCATA12800.039.19922330
ATACTAT16000.039.0937545
AGGGCGA25800.038.9825556
CACGACC14750.038.89830827
ACGACCA15000.038.39999828
TTACGGG5100.038.3823553
GCGATAT2900.038.0172429
GCCCTAC17200.037.80523326
AGACACG15250.037.77049324
GTTGATC13300.037.72556316
TATGGGA15850.037.6182984
TAGGGAC13900.037.5539555
Produced by FastQC (version 0.11.5)