##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546998_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2105447
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59954536970059	31.0	31.0	33.0	30.0	34.0
2	31.99411193917491	31.0	31.0	34.0	30.0	34.0
3	32.111894528810275	33.0	31.0	34.0	30.0	34.0
4	35.795387392795924	37.0	35.0	37.0	35.0	37.0
5	35.74002100266594	37.0	35.0	37.0	33.0	37.0
6	35.790313410881396	37.0	35.0	37.0	35.0	37.0
7	36.08883101783137	37.0	35.0	37.0	35.0	37.0
8	36.01547699847111	37.0	35.0	37.0	35.0	37.0
9	37.795524180850904	39.0	38.0	39.0	35.0	39.0
10	37.34688168355699	39.0	37.0	39.0	34.0	39.0
11	37.08998421712824	39.0	37.0	39.0	33.0	39.0
12	36.60192111223888	39.0	35.0	39.0	33.0	39.0
13	36.46287415451446	39.0	35.0	39.0	32.0	39.0
14	37.49023983980599	40.0	35.0	41.0	32.0	41.0
15	37.62345573172823	40.0	35.0	41.0	33.0	41.0
16	37.7187314617751	40.0	35.0	41.0	33.0	41.0
17	37.660909061116236	40.0	35.0	41.0	33.0	41.0
18	37.60821716243629	39.0	36.0	41.0	33.0	41.0
19	37.54225634746446	39.0	35.0	41.0	33.0	41.0
20	37.4050987747495	39.0	35.0	41.0	32.0	41.0
21	37.3448056398475	39.0	35.0	41.0	32.0	41.0
22	37.275571410726556	39.0	35.0	41.0	32.0	41.0
23	37.183144481908116	39.0	35.0	41.0	32.0	41.0
24	37.06920953127768	39.0	35.0	41.0	32.0	41.0
25	36.92095787735336	39.0	35.0	41.0	32.0	41.0
26	36.7718299249518	38.0	35.0	41.0	31.0	41.0
27	36.674530396633116	38.0	35.0	41.0	31.0	41.0
28	36.70999222492896	38.0	35.0	41.0	31.0	41.0
29	36.69610301280441	38.0	35.0	41.0	31.0	41.0
30	36.5977395773914	38.0	35.0	41.0	31.0	41.0
31	36.46388011666881	38.0	35.0	41.0	31.0	41.0
32	36.39107609927963	38.0	35.0	41.0	30.0	41.0
33	36.27762465642688	38.0	35.0	41.0	30.0	41.0
34	36.08254541672149	38.0	35.0	41.0	30.0	41.0
35	35.99621410560323	38.0	35.0	41.0	30.0	41.0
36	35.83949679094273	38.0	35.0	41.0	29.0	41.0
37	35.75624416097864	38.0	35.0	41.0	29.0	41.0
38	35.63639455184576	38.0	35.0	40.0	29.0	41.0
39	35.53478050029281	38.0	35.0	40.0	28.0	41.0
40	35.36352945479036	38.0	34.0	40.0	27.0	41.0
41	35.28647218381655	37.0	34.0	40.0	27.0	41.0
42	35.18891475301919	37.0	34.0	40.0	27.0	41.0
43	35.069644593285894	37.0	34.0	40.0	27.0	41.0
44	34.841627454882506	37.0	34.0	40.0	26.0	41.0
45	34.7516969080675	36.0	34.0	40.0	26.0	41.0
46	34.70492299259967	36.0	34.0	40.0	26.0	41.0
47	34.65030798685505	36.0	34.0	40.0	26.0	41.0
48	34.5655497383691	36.0	34.0	40.0	26.0	41.0
49	34.46110635888721	36.0	34.0	40.0	26.0	41.0
50	34.34642524841518	35.0	34.0	40.0	26.0	41.0
51	33.96888119244987	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	3.0
12	5.0
13	6.0
14	14.0
15	63.0
16	159.0
17	369.0
18	778.0
19	1609.0
20	2788.0
21	4547.0
22	6667.0
23	9840.0
24	14410.0
25	20417.0
26	26780.0
27	32092.0
28	36140.0
29	40565.0
30	47374.0
31	57076.0
32	70146.0
33	92081.0
34	159179.0
35	244568.0
36	167956.0
37	218629.0
38	309687.0
39	541096.0
40	398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.45206647329522	24.35506569388828	27.94285489019671	13.250012942619785
2	31.401170392795446	25.803119242612137	28.50159609812073	14.29411426647168
3	29.96636818689808	25.4453804821494	30.288817529009282	14.299433801943245
4	25.68361967791163	29.07016894749666	29.94243027727604	15.303781097315678
5	23.39460456615626	32.92398241323576	28.39363802555942	15.287774995048558
6	22.210532965208813	42.09063443534794	25.039813398294996	10.65901920114826
7	88.28809274230129	3.6502462422468955	6.343213578874225	1.7184474365776008
8	88.24487151659481	3.1207624794164848	5.84317724454712	2.7911887594415816
9	82.76755482327506	4.818311740927224	8.797561752919925	3.616571682877793
10	47.17872261804738	23.348628581009162	15.084255267408775	14.388393533534686
11	36.70688457130481	23.891981132747585	21.631178557332483	17.769955738615124
12	31.558524151878437	20.32475764053904	27.627624917654064	20.489093289928455
13	23.700192880656694	27.943472336278237	29.131153622009958	19.22518116105511
14	20.634573085905274	28.54980438833179	31.122797201734357	19.69282532402858
15	20.372443476373427	21.470310105170064	38.080654606836454	20.07659181162005
16	23.43350366929208	19.452923773431486	34.65297392905165	22.46059862822479
17	22.95645532753852	21.083693866433116	27.672033539671148	28.28781726635722
18	25.338514814193854	21.23192842185056	30.333796101255455	23.09576066270013
19	28.028394920413575	24.56960445929059	25.1300555179019	22.271945102393932
20	29.191520850441737	23.869373106993432	24.58195338092101	22.357152661643823
21	26.388315640336707	25.119748917925744	26.885834694485304	21.606100747252245
22	26.089756711995125	22.324570506880487	25.446140415788193	26.139532365336198
23	25.01834527299904	25.3499613146282	24.718598948346834	24.913094464025928
24	23.882244483000523	23.51457908938102	31.094917136361065	21.508259291257392
25	24.451767249424943	23.556375439514746	28.30477328567283	23.687084025387485
26	22.637283199244624	26.268483604669225	25.831996720886348	25.2622364751998
27	23.065458308853177	24.897040865906384	26.895713831789642	25.1417869934508
28	21.841300208459298	25.72247128519502	28.891632038232263	23.544596468113422
29	22.63277109326428	23.66799069271276	28.50444584926621	25.194792364756747
30	24.641180708894595	25.094956082960056	28.11336500040134	22.15049820774401
31	27.626675000605573	24.776876359271927	24.56195762705022	23.034491013072284
32	28.77070759795901	23.838833273884358	24.086666631836376	23.30379249632026
33	27.792198046305604	23.52441073083293	23.93482239163465	24.748568831226812
34	23.02351947116218	25.331770403149545	27.072778369628875	24.5719317560594
35	24.230531568830752	25.536240047837822	26.14523186762716	24.087996515704265
36	27.85674490975076	25.326593355235254	23.415692724632823	23.400969010381168
37	24.1363472934726	27.241531133293783	24.75056365702865	23.871557916204967
38	24.701547937326374	26.096358635482158	24.775166508584636	24.426926918606835
39	23.608383397919777	26.210871135678076	23.50394001843789	26.67680544796426
40	26.298975941925875	23.462856106090534	25.45126996785006	24.786897984133535
41	22.139669153391182	23.365774583734474	25.72722087043749	28.767335392436856
42	23.979420997061432	24.423792192346802	25.306027651135366	26.2907591594564
43	23.424954415855638	22.907439607836245	27.573717125152047	26.093888851156073
44	23.691881106482377	24.77369413715947	26.33208055106588	25.20234420529227
45	22.14755346489368	25.664906312056303	25.154563377753036	27.032976845296986
46	24.058929054020357	24.955508260241174	26.19030543157819	24.79525725416028
47	23.424526953183815	24.217565201118813	27.63484428722262	24.723063558474756
48	24.02135983475243	23.3579377680844	27.68632979125098	24.934372605912188
49	23.821259808487223	22.80565599609014	28.130843474093624	25.24224072132901
50	22.97611861044234	25.299473223500758	27.109540159405583	24.61486800665132
51	22.199703910855984	25.997519766586386	25.808866240755524	25.993910081802106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	998.0
1	1804.0
2	2610.0
3	9660.0
4	16710.0
5	11717.0
6	6724.0
7	6837.5
8	6951.0
9	7424.5
10	7898.0
11	8015.5
12	8133.0
13	8208.5
14	8284.0
15	8268.0
16	8252.0
17	7943.0
18	7634.0
19	7475.0
20	7316.0
21	7475.0
22	7634.0
23	7893.0
24	8152.0
25	8496.5
26	10380.0
27	11919.0
28	13517.5
29	15116.0
30	17626.5
31	20137.0
32	24240.0
33	28343.0
34	34313.0
35	40283.0
36	42162.0
37	44041.0
38	49709.5
39	55378.0
40	68549.5
41	81721.0
42	91846.0
43	101971.0
44	111195.5
45	120420.0
46	125021.5
47	129623.0
48	143750.5
49	157878.0
50	164137.0
51	170396.0
52	166872.5
53	163349.0
54	151420.0
55	139491.0
56	132601.5
57	125712.0
58	123527.5
59	121343.0
60	113928.0
61	106513.0
62	100155.0
63	93797.0
64	84567.5
65	75338.0
66	67671.0
67	60004.0
68	51817.0
69	43630.0
70	38198.0
71	32766.0
72	28234.0
73	23702.0
74	19750.0
75	12743.5
76	9689.0
77	7583.0
78	5477.0
79	4267.5
80	3058.0
81	2126.5
82	1195.0
83	918.5
84	642.0
85	520.0
86	398.0
87	246.5
88	95.0
89	76.5
90	58.0
91	38.5
92	19.0
93	13.5
94	8.0
95	4.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2105447.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.739125758548997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.11966143212842	16.982531440883093
2	7.162012501271955	3.113917808989022
3	2.495113564905477	1.62724762708025
4	1.3408384604841885	1.1659462365746003
5	0.8752762771037046	0.9513870530716007
6	0.6825038369657724	0.8902222045494688
7	0.5353950378721493	0.814731404116403
8	0.42526309463958195	0.7395878315871677
9	0.37400204312015506	0.7317429704408984
>10	5.420116934343917	29.89971831894408
>50	2.361093891346853	35.755460699563955
>100	0.20407972702141342	5.8449035463423265
>500	0.0019899423413353326	0.26335487301667193
>1k	0.0024321517505209622	0.7559375958994705
>5k	2.2110470459281472E-4	0.46331038894099297
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9639	0.4578125215215581	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.7495852424687015E-5	0.0	0.0	0.09009963204963127	0.0
2	4.7495852424687015E-5	0.0	0.0	0.2699189293294963	0.0
3	4.7495852424687015E-5	0.0	0.0	0.39825272258100064	0.0
4	4.7495852424687015E-5	0.0	0.0	0.668314139467771	0.0
5	4.7495852424687015E-5	0.0	0.0	1.0551678574668466	0.0
6	4.7495852424687015E-5	0.0	0.0	1.8017076658780773	0.0
7	4.7495852424687015E-5	0.0	0.0	2.134083641146037	0.0
8	4.7495852424687015E-5	0.0	0.0	3.1175327614516064	0.0
9	4.7495852424687015E-5	0.0	0.0	3.5705007060258462	0.0
10	4.7495852424687015E-5	0.0	0.0	4.245654248242772	0.0
11	4.7495852424687015E-5	0.0	0.0	4.855311009966055	0.0
12	4.7495852424687015E-5	0.0	0.0	5.333071789505982	0.0
13	4.7495852424687015E-5	0.0	0.0	5.573923257151569	0.0
14	4.7495852424687015E-5	0.0	0.0	5.697364977603331	0.0
15	4.7495852424687015E-5	0.0	0.0	5.824701357953916	0.0
16	4.7495852424687015E-5	0.0	0.0	6.055008746361224	0.0
17	4.7495852424687015E-5	0.0	0.0	6.305216896934475	0.0
18	4.7495852424687015E-5	0.0	0.0	6.655546304418967	0.0
19	4.7495852424687015E-5	0.0	0.0	6.82187677961022	0.0
20	4.7495852424687015E-5	0.0	0.0	7.000508680579468	0.0
21	4.7495852424687015E-5	0.0	0.0	7.2195120561097	0.0
22	4.7495852424687015E-5	0.0	0.0	7.452241732990666	0.0
23	4.7495852424687015E-5	0.0	0.0	7.709574261427621	0.0
24	4.7495852424687015E-5	0.0	0.0	7.921453259094149	0.0
25	4.7495852424687015E-5	0.0	0.0	8.102697431946755	0.0
26	4.7495852424687015E-5	0.0	0.0	8.265750693320706	0.0
27	4.7495852424687015E-5	0.0	0.0	8.45093702192456	0.0
28	4.7495852424687015E-5	0.0	0.0	8.616032604952773	0.0
29	4.7495852424687015E-5	0.0	0.0	8.813045400810374	0.0
30	4.7495852424687015E-5	0.0	0.0	9.089281278512354	0.0
31	4.7495852424687015E-5	0.0	0.0	9.280214605259596	0.0
32	4.7495852424687015E-5	0.0	0.0	9.478604780837513	0.0
33	4.7495852424687015E-5	0.0	0.0	9.679797211708488	0.0
34	4.7495852424687015E-5	0.0	0.0	9.880324700645517	0.0
35	4.7495852424687015E-5	0.0	0.0	10.129012983941177	0.0
36	4.7495852424687015E-5	0.0	0.0	10.326785713437575	0.0
37	4.7495852424687015E-5	0.0	0.0	10.52992547425796	0.0012348921630418624
38	9.499170484937403E-5	0.0	0.0	10.777426361243004	0.0012348921630418624
39	9.499170484937403E-5	0.0	0.0	11.20522150403216	0.0012348921630418624
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAA	275	0.0	41.727272	9
CGTAAGG	390	0.0	41.53846	2
CGTTTTT	5980	0.0	41.049335	1
GGCGATA	1155	0.0	39.935066	8
CATATGC	1230	0.0	39.512196	33
AGGGTAC	1025	0.0	39.51219	6
GGGCGAT	4585	0.0	39.40567	7
GCGTAAG	275	0.0	39.272728	1
TAGCATA	1280	0.0	39.199223	30
ATACTAT	1600	0.0	39.09375	45
AGGGCGA	2580	0.0	38.982555	6
CACGACC	1475	0.0	38.898308	27
ACGACCA	1500	0.0	38.399998	28
TTACGGG	510	0.0	38.382355	3
GCGATAT	290	0.0	38.017242	9
GCCCTAC	1720	0.0	37.805233	26
AGACACG	1525	0.0	37.770493	24
GTTGATC	1330	0.0	37.725563	16
TATGGGA	1585	0.0	37.618298	4
TAGGGAC	1390	0.0	37.553955	5
>>END_MODULE