FastQCFastQC Report
Thu 26 May 2016
SRR1546997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546997_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2384685
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142850.5990308992592313No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91900.041.0582161
GCGTAAG1300.039.807691
CGTAAGG3250.038.769232
ACTATCG356.2511153E-638.57142638
TACGGGT5600.038.5714264
TCACGAC659.094947E-1238.0769225
TAGGGAC15850.037.9022065
ACGTAGG3800.037.8947372
TCGTTAG1550.037.7419361
ATAGGGT16900.037.5443764
ACGTACG301.1402897E-437.5000041
TAGTACG900.037.51
AACGGGA4700.037.3404274
AGGGCGA34600.037.261566
AGGGTAC17750.037.0140846
TAGGGTA16200.036.8055535
GGCGATA11500.036.7826088
TAGGGTC14250.036.631585
GGGCGAT65250.036.586217
ATAGGGA19000.036.236844
Produced by FastQC (version 0.11.5)