##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546997_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2384685
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.711932183915277	31.0	31.0	33.0	30.0	34.0
2	32.11998188439983	33.0	31.0	34.0	30.0	34.0
3	32.225834020006836	33.0	31.0	34.0	30.0	34.0
4	35.905244927527114	37.0	35.0	37.0	35.0	37.0
5	35.87295009613429	37.0	35.0	37.0	35.0	37.0
6	35.931474387602556	37.0	35.0	37.0	35.0	37.0
7	36.20095400440729	37.0	35.0	37.0	35.0	37.0
8	36.14100856087911	37.0	35.0	37.0	35.0	37.0
9	37.92858134302853	39.0	38.0	39.0	35.0	39.0
10	37.40022854171515	39.0	37.0	39.0	34.0	39.0
11	37.2397104858713	39.0	37.0	39.0	34.0	39.0
12	36.92285186513104	39.0	35.0	39.0	33.0	39.0
13	36.849205240943775	39.0	35.0	39.0	33.0	39.0
14	37.98587570266094	40.0	37.0	41.0	33.0	41.0
15	38.062011544501686	40.0	36.0	41.0	33.0	41.0
16	38.11670681872029	40.0	36.0	41.0	33.0	41.0
17	38.06769321734317	40.0	36.0	41.0	33.0	41.0
18	37.97211707206612	40.0	36.0	41.0	33.0	41.0
19	37.914585783866634	40.0	36.0	41.0	33.0	41.0
20	37.772700796960606	40.0	35.0	41.0	33.0	41.0
21	37.72420592237549	40.0	35.0	41.0	33.0	41.0
22	37.63289952341714	40.0	35.0	41.0	33.0	41.0
23	37.521449583487964	39.0	35.0	41.0	33.0	41.0
24	37.445962464644175	39.0	35.0	41.0	33.0	41.0
25	37.31301324912934	39.0	35.0	41.0	32.0	41.0
26	37.19409104347115	39.0	35.0	41.0	32.0	41.0
27	37.11208608264823	39.0	35.0	41.0	32.0	41.0
28	37.13447142914054	39.0	35.0	41.0	32.0	41.0
29	37.12722267301552	39.0	35.0	41.0	32.0	41.0
30	37.037595741156586	39.0	35.0	41.0	32.0	41.0
31	36.92919274453439	39.0	35.0	41.0	32.0	41.0
32	36.82796176434204	39.0	35.0	41.0	31.0	41.0
33	36.67572530543866	39.0	35.0	41.0	31.0	41.0
34	36.48424508897401	39.0	35.0	41.0	30.0	41.0
35	36.34378586689647	39.0	35.0	41.0	30.0	41.0
36	36.246711410521726	39.0	35.0	41.0	30.0	41.0
37	36.14008894256474	39.0	35.0	41.0	30.0	41.0
38	36.03569737722173	39.0	35.0	41.0	30.0	41.0
39	35.90827216173205	38.0	35.0	41.0	29.0	41.0
40	35.779721430713074	38.0	35.0	41.0	29.0	41.0
41	35.703770099614836	38.0	35.0	41.0	29.0	41.0
42	35.61865110066948	38.0	35.0	41.0	28.0	41.0
43	35.52925816197946	38.0	35.0	40.0	28.0	41.0
44	35.384521226073886	38.0	35.0	40.0	27.0	41.0
45	35.26925526851555	38.0	34.0	40.0	27.0	41.0
46	35.21087481155792	37.0	34.0	40.0	27.0	41.0
47	35.14832692787517	37.0	34.0	40.0	27.0	41.0
48	35.05983851116604	37.0	34.0	40.0	27.0	41.0
49	34.95330913726551	37.0	34.0	40.0	27.0	41.0
50	34.85192803242357	37.0	34.0	40.0	26.0	41.0
51	34.480782577153796	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	9.0
13	10.0
14	19.0
15	48.0
16	132.0
17	308.0
18	691.0
19	1350.0
20	2448.0
21	4012.0
22	6138.0
23	9607.0
24	14171.0
25	21285.0
26	28927.0
27	34095.0
28	36756.0
29	40023.0
30	45877.0
31	55366.0
32	68943.0
33	91594.0
34	159386.0
35	242522.0
36	185031.0
37	245260.0
38	371025.0
39	719184.0
40	462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.07351495061193	23.976625843664888	27.388606880992665	14.56125232473052
2	32.90472326533693	26.27055564990764	27.87693133474652	12.947789750008912
3	30.118443316412858	25.734593877178746	30.550911336298086	13.596051470110307
4	25.796027567582303	28.510264458408553	30.39558683851326	15.298121135495881
5	23.48012420927712	32.40356692812678	29.17592889626932	14.94037996632679
6	22.591788852615753	41.372424450189435	25.82949110679188	10.206295590402926
7	89.0147755363916	3.032643724433206	6.5803659602840625	1.3722147788911323
8	88.72698071233727	2.6882795840960125	6.557721460067052	2.0270182434996653
9	83.26252733589553	4.171871756647104	9.372432837041371	3.193168070416009
10	40.375730966563715	23.158530371935914	22.721114109410674	13.74462455208969
11	33.80576470267562	26.123953478132332	22.900802412058617	17.169479407133437
12	28.596984507387766	23.32379328926043	28.00399214151974	20.075230061832066
13	23.847929600764882	26.541534835837854	30.55187582427029	19.05865973912697
14	22.090003501510683	28.017369170351643	30.954444717017132	18.938182611120546
15	21.892618941285747	23.341657283876067	35.85173723154212	18.913986543296073
16	24.438363976793582	21.647764799124413	33.80647758508986	20.107393638992153
17	24.375714192859853	21.934133858350265	29.00227912701258	24.687872821777297
18	24.930462513916932	22.804479417617003	30.900559193352585	21.364498875113487
19	27.516128964622162	24.470527553953666	27.192102940220614	20.821240541203558
20	28.72148732432166	24.48021436793539	26.157668622899884	20.64062968484307
21	27.293374177302244	24.92660456202811	28.088154200659627	19.691867060010022
22	26.248288558027582	22.556815680058374	27.93861663070804	23.25627913120601
23	24.09525786424622	26.236043754206527	27.336692267532193	22.33200611401506
24	23.56013477671055	24.788682782002656	31.16738688757635	20.48379555371045
25	23.668325166636265	24.602997880223175	29.765189113027507	21.963487840113054
26	21.762329196518618	27.170213256677506	27.96620937356506	23.101248173238815
27	22.476763178365278	25.973535288727863	28.460446557931128	23.08925497497573
28	21.800489372810244	26.39283595107949	30.63947649270239	21.16719818340787
29	22.53811300024951	24.325938226642094	30.120498095136256	23.015450677972144
30	23.893889549353478	24.849739064069258	30.072860776161214	21.18351061041605
31	26.052916842266377	25.17716176350336	26.72285857461258	22.047062819617686
32	27.315683203441964	24.886389607013086	26.61206826058788	21.185858928957074
33	26.24115973388519	24.764151240100894	26.358701463715335	22.635987562298585
34	23.8760674889975	25.949171483864745	28.142668738219097	22.032092288918662
35	23.958216703673653	25.840897225419713	28.682194923019182	21.518691147887456
36	27.864392991107838	25.925688298454514	25.45472462820037	20.755194082237278
37	24.778995968020933	27.111211753334295	26.816455842176218	21.29333643646855
38	25.674418214565026	27.06042936488467	26.049478232974167	21.215674187576138
39	24.461302016828217	26.998115055028233	25.975673935970576	22.56490899217297
40	25.68670495264574	25.379201026550675	27.137294862843518	21.796799157960066
41	22.697924463818072	25.068719768019676	27.78933066631442	24.44402510184783
42	24.317132032113257	25.846055139358025	26.343143853381058	23.49366897514766
43	23.484191832464248	24.861941933630646	27.810465533183624	23.843400700721478
44	23.51220391791788	25.97303207761193	27.999756781293968	22.515007223176227
45	22.622149256610413	26.438166885773175	27.209925000576597	23.72975885703982
46	23.68300215751766	26.274161996238497	27.52577384434422	22.51706200189962
47	23.458569999811296	25.301622646177584	28.61564525293697	22.62416210107415
48	23.81421445599733	25.18575828673389	28.69553001759142	22.304497239677357
49	23.588314599202832	25.128601890815766	28.513954673258734	22.76912883672267
50	23.175891155435625	26.138043389378474	27.934632876040233	22.75143257914567
51	22.863690592258518	26.909507964364266	27.1099117912848	23.116889652092414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1244.0
1	2191.5
2	3139.0
3	14025.5
4	24912.0
5	17477.0
6	10042.0
7	10077.5
8	10113.0
9	10670.0
10	11227.0
11	11381.0
12	11535.0
13	11411.5
14	11288.0
15	10928.0
16	10568.0
17	10495.0
18	10422.0
19	10579.0
20	10736.0
21	10679.5
22	10623.0
23	11009.0
24	11395.0
25	13411.0
26	16340.0
27	17253.0
28	21855.0
29	26457.0
30	30901.0
31	35345.0
32	41437.5
33	47530.0
34	54168.5
35	60807.0
36	63899.5
37	66992.0
38	75798.5
39	84605.0
40	97484.5
41	110364.0
42	119590.5
43	128817.0
44	134709.0
45	140601.0
46	147993.0
47	155385.0
48	161325.5
49	167266.0
50	174813.0
51	182360.0
52	177239.0
53	172118.0
54	162261.0
55	152404.0
56	140386.0
57	128368.0
58	121065.5
59	113763.0
60	108412.0
61	103061.0
62	95611.5
63	88162.0
64	79484.0
65	70806.0
66	62671.0
67	54536.0
68	47272.0
69	40008.0
70	34425.5
71	28843.0
72	25020.5
73	21198.0
74	18242.0
75	12576.5
76	9867.0
77	7396.0
78	4925.0
79	3818.5
80	2712.0
81	1891.0
82	1070.0
83	849.0
84	628.0
85	446.5
86	265.0
87	172.5
88	80.0
89	79.5
90	79.0
91	53.5
92	28.0
93	24.5
94	21.0
95	12.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2384685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.374574127783085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.865352549	16.429641759167808
2	6.525883616467048	2.7897596621892014
3	2.4069772216622445	1.5434433914491505
4	1.3539117382896302	1.1575714725018937
5	0.9422221441505044	1.0069798532491836
6	0.7305012691992655	0.9368492117363597
7	0.603908920953051	0.9035807198138339
8	0.5206628899474119	0.8903158029413377
9	0.44033819098215354	0.8470837173997751
>10	7.461448404996415	40.96974923170263
>50	2.0547318303288384	28.70379080744731
>100	0.09069486402406982	2.67673437813101
>500	0.0025742752936726626	0.39155952028454893
>1k	5.94063529309076E-4	0.148310945743926
>5k	0.0	0.0
>10k+	1.9802117643635863E-4	0.6046295262420043
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14285	0.5990308992592313	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045037394876052815	0.0
2	0.0	0.0	0.0	0.13628634389867006	0.0
3	0.0	0.0	0.0	0.21667431966905482	0.0
4	0.0	0.0	0.0	0.362731346068768	0.0
5	0.0	0.0	0.0	0.5770992814564607	0.0
6	0.0	0.0	0.0	1.0465533183628026	0.0
7	0.0	0.0	0.0	1.2537504953484422	0.0
8	0.0	0.0	0.0	1.8267821536177735	0.0
9	0.0	0.0	0.0	2.141708443672854	0.0
10	0.0	0.0	0.0	2.6226105334666845	0.0
11	0.0	0.0	0.0	3.02647938826302	0.0
12	0.0	0.0	0.0	3.361701859994087	0.0
13	0.0	0.0	0.0	3.543990086741016	0.0
14	0.0	0.0	0.0	3.647567708104005	0.0
15	0.0	0.0	0.0	3.743513294208669	0.0
16	4.193425966112925E-5	0.0	0.0	3.9078117235609735	0.0
17	4.193425966112925E-5	0.0	0.0	4.072864969587179	0.0
18	4.193425966112925E-5	0.0	0.0	4.286352285521987	0.0
19	4.193425966112925E-5	0.0	0.0	4.403642409794165	0.0
20	4.193425966112925E-5	0.0	0.0	4.5333870091857	0.0
21	4.193425966112925E-5	0.0	0.0	4.688879244009167	0.0
22	4.193425966112925E-5	0.0	0.0	4.8490681159146805	0.0
23	4.193425966112925E-5	0.0	0.0	5.025108137972101	0.0
24	4.193425966112925E-5	0.0	0.0	5.168397503234179	0.0
25	4.193425966112925E-5	0.0	0.0	5.296338929460285	0.0
26	4.193425966112925E-5	0.0	0.0	5.416396714870098	0.0
27	4.193425966112925E-5	0.0	0.0	5.552179847652835	0.0
28	4.193425966112925E-5	0.0	0.0	5.682092184083013	0.0
29	4.193425966112925E-5	0.0	0.0	5.827352459549164	0.0
30	4.193425966112925E-5	0.0	0.0	6.037778574528711	0.0
31	4.193425966112925E-5	0.0	0.0	6.190964425070817	0.0
32	4.193425966112925E-5	0.0	0.0	6.350859757158703	0.0
33	4.193425966112925E-5	0.0	0.0	6.505345569750302	0.0
34	4.193425966112925E-5	0.0	0.0	6.658866894369696	0.0
35	4.193425966112925E-5	0.0	0.0	6.829874805267782	0.0
36	4.193425966112925E-5	0.0	0.0	6.9853251058315875	0.0
37	4.193425966112925E-5	0.0	0.0	7.151929919465254	0.0
38	4.193425966112925E-5	0.0	0.0	7.339585731448808	0.0
39	4.193425966112925E-5	0.0	0.0	7.600165220983065	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9190	0.0	41.058216	1
GCGTAAG	130	0.0	39.80769	1
CGTAAGG	325	0.0	38.76923	2
ACTATCG	35	6.2511153E-6	38.571426	38
TACGGGT	560	0.0	38.571426	4
TCACGAC	65	9.094947E-12	38.07692	25
TAGGGAC	1585	0.0	37.902206	5
ACGTAGG	380	0.0	37.894737	2
TCGTTAG	155	0.0	37.741936	1
ATAGGGT	1690	0.0	37.544376	4
ACGTACG	30	1.1402897E-4	37.500004	1
TAGTACG	90	0.0	37.5	1
AACGGGA	470	0.0	37.340427	4
AGGGCGA	3460	0.0	37.26156	6
AGGGTAC	1775	0.0	37.014084	6
TAGGGTA	1620	0.0	36.805553	5
GGCGATA	1150	0.0	36.782608	8
TAGGGTC	1425	0.0	36.63158	5
GGGCGAT	6525	0.0	36.58621	7
ATAGGGA	1900	0.0	36.23684	4
>>END_MODULE