FastQCFastQC Report
Thu 26 May 2016
SRR1546996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546996_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1481995
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280781.894608281404458No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT22700.15317190678780968No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC18770.12665359869635187No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT16280.10985192257733661No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT16100.10863734358078131No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG15710.10600575575491146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG700.045.00000421
CTACGCG253.891354E-545.01
CGTTTTT219000.044.3424641
GCTAGCG700.041.7857171
ACGGGTA1350.041.6666645
TGTAGCG1400.040.1785741
CTACGGG1800.040.03
CGTGCGG850.039.7058832
GCCGCAT850.039.70588319
AACGCCG850.039.70588313
CGCCGCA850.039.70588318
CGTTAGG1650.039.5454522
GTTTTTT246750.039.382982
TCGCCAG800.039.37525
AACGCGC403.4591176E-739.37514
CGCCAGT750.039.00000426
GGCCGAT4050.038.8888858
TACGGGA4700.038.7765964
TTAGGGA16450.038.1610954
CGTTCTG2250.038.0000041
Produced by FastQC (version 0.11.5)