FastQCFastQC Report
Thu 26 May 2016
SRR1546995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546995_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1734989
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92350.532280031746599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCG501.0822987E-940.51
CGTTTTT56400.040.4122351
AGGGCGA22550.038.813756
CGTAAGG2500.038.6999972
TAACGCC700.038.5714312
GCGATAC700.038.571439
GGGCGAT45150.038.3222587
GTCGTAG659.094947E-1238.076921
GCGTAAG1600.037.968751
GCGATGT4650.036.7741939
GGCGATT11900.036.4915968
CAATTCG250.002107404236.021
CGTAATC250.002107404236.038
TCGGTAA250.002107404236.037
CGGTAGT4200.035.89285712
GTAGGGC10100.035.8663334
TAGGGCG8950.035.6983265
GCCAGTA10150.035.6896625
TCGTTAG950.035.5263141
GCGATCG1400.035.3571439
Produced by FastQC (version 0.11.5)